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Showing 1 to 3 of 3 for “"Protein production & purification"”.
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Biomimicry of the spider silk spinning apparatus
… literature there is no detailed protocol on the production of consistent synthetic fibers. To accomplish this, the fibroins and natural spinning apparatus were taken apart and analyzed in order to develop a protocol that biomimics the spider's system. The laboratory procedure, using the natural …
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Aggregation of IgG mAb Biotherapeutics: Sources, Methods of Characterization, and Biological Implications
One of the predominant concerns with protein therapeutics is their tendency to aggregate at various stages of protein production, purification, filling, transportation, and administration. This occurrence has biological significance; while there is no definite, general cause and effect relationship …
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Recombinant Expression In Pichia pastoris System Of The Potassium Channel Inhibitor Peptide, Ts6.
… of ZeocinTM. After methanol induction, protein expression yielded around 4.7 mg/l Ts6. The purification of the his-tag Ts6 was done in two steps that included the Ni2+-NTA affinity chromatography then followed by reversed-phase-HPLC. Protein was quantified using bicinchoninic acid (BCA) …