Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 36 for “"Protein p53"”.
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Novel RNA-based biomarkers for ovarian cancer – uncovering how LINC01132 is regulated by the tumour suppressor protein, p53
… 60% of ovarian cancer cases have a mutation in p53, a tumour suppressor transcription factor which counteracts cell stress and oncogenic signals. Most mutations occur within p53’s DNA-binding domain, a vital region which facilitates its anchorage to target gene promoters. Previous data found …
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The detection and clinical significance of TP53 dysfunction in chronic lymphocytic leukaemia.
… apoptosis. Central to this response is the protein, p53. Loss of the chromosome containing the gene TP53, which encodes the protein p53 and/or mutation of the gene, occurs in approximately 5-10% of newly diagnosed CLL patients. This rises to over 30% in patients who relapse after therapy. In …
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Using Stable Limit Cycles to Model p53-Mdm2 Protein Interactions in the Presence of DNA Damage
The protein p53 is constantly made and destroyed within a cell. Another protein, Mdm2, is a negative regulator of p53. In the presence of DNA damage, Mdm2 does not bind to p53, thus activating the p53 protein. This activation then promotes the mechanisms necessary to either fix or terminate the …
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p53 in a Genetic Model: Illuminating Adaptive Radiation Responses
… by genotoxic stress, the tumor suppressor protein p53 promotes adaptive responses, including cell cycle arrest, DNA repair, or apoptosis. How these distinct fates are specified through an action of a single protein is not known. To study its functions in vivo we produced a targeted mutation …
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SERS-active peptide nanoparticles for investigating protein allostery and bionanoassembly
The tumour suppressor protein, p53, is either mutated or absent in over 50 % of cancers and is negatively regulated by the mouse double minute two protein, MDM2. This thesis presents a novel nanosensing approach to investigate full-length MDM2 interactions with p53, thus providing an allosteric …
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Towards the Understanding of Fluorescence Quenching Mechanisms : Molecular Dynamics Simulations of Dye-Quencher Interactions in Biomolecular Systems
The presence of antibodies directed against p53 in human blood serum is a specific and independent marker of cancer. The present thesis is a simulative study of two fluorescent dyes (rhodamine 6G and MR121) used in the development of fluorescence-based immunoassays for the detection of p53 …
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Phosphoserine Incorporation into the Intrinsically Disordered N-terminal Domain of the p53 Tumor Suppressor Protein
Protein phosphorylation is used biologically as a mechanism to control many critical life processes. Since phosphorylation usually occurs within protein regions that are intrinsically disordered, quantitative and structural descriptions of phosphorylation effects in intrinsically disordered …
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Novel Role of CHK2 in the Intrinsic and Extrinsic Apoptosis Pathway
… is an evolutionarily conserved serine/threonine protein kinase that is activated in response to DNA double strand breaks. Upon activation by the DNA damage transducer kinase, ATM, CHK2 phosphorylates a wide variety of down stream effectors to promote cell cycle arrest, DNA repair and apoptosis. …
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Circadian Control of Cell Cycle Progression
… that Per2 interacts with the tumor suppressor protein p53. Both G1 and G2 checkpoint pathways involve a p53 dependent pathway which can trigger the cell to go through cell arrest or programmed cell death4. Understanding all the mitigating factors involved in regulating cell cycle progression …
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Interplay Between P53 and Epigenetic Pathways in Cancer
The human TP53 gene encodes the most potent tumor suppressor protein p53. More than half of all human cancers contain mutations in the TP53 gene, while the majority of the remaining cases involve other mechanisms to inactivate wild-type p53 function. In the first part of my dissertation research, I …
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Copper-64 radiopharmaceuticals for receptor-mediated tumor imaging and radiotherapy
… 64Cu-DOTA-cetuximab, the tumor suppressor protein p53 was identified as a mediator of the nuclear transport of copper. 64Cu-DOTA-cetuximab was also utilized in five cervical cancer cell lines with a wide range of EGFR expression. EGFR quantification by saturation receptor binding, and EGFR …
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MUTANT P53 AS A THERAPEUTIC TARGET IN CANCER
Identifying therapeutic approaches to target TP53 mutations has the potential to revolutionize cancer treatment. TP53, the gene that encodes the prominent tumor suppressor protein p53, is mutated and inactivated in half of sporadic human tumors. Additionally, individuals who harbor germline TP53 …
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Function and Properties of groE Chaperonins in Bacterial and Mammalian Cells
… most polypeptides <em>in vivo</em>, and protect proteins against aggregation when a cell is under stress. The GroESL proteins of <em>Escherichia coli</em> are the best characterized of the ringed chaperones, or chaperonins. Chaperonins of the eukaryotic cytoplasm interact with a limited number of …
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Phospho-regulation and metastatic potential of Murine Double Minute 2
… (Mdm2) is a highly modified and multi-faceted protein that is overexpressed in numerous human malignancies. It engages in many cellular activities and is essential for development since deletion of mdm2 is lethal in early stages of embryonic development. The most studied function of Mdm2 is as …
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Role of the RING domain in MDM2-mediated ubiquitination of p53
The MDM2 protein regulates the tumour suppressor protein p53, acting as its chaperone, regulating its translation and targeting p53 for degradation by the 26s proteasome via its E3 ligase activity. The E3 ligase activity of MDM2 is dependent on its C-terminal RING domain. E3 ligases containing a …
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Investigation of physical principles of protein phase transitions
Protein phase transitions, including liquid-to-solid aggregation and liquid-liquid phase separation, are fundamental processes underlying neurodegenerative disorders such as Alzheimer’s (the Aβ peptide and the tau protein), Parkinson’s (the α-synuclein protein), and Huntington’s diseases (the Htt …
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Neurological Consequences of Viral Encephalitis: Behavioral Deficits, Neuronal Restructuring, and Therapeutic Interventions
… focus of this study is the tumor suppressor protein p53, a pivotal regulator of apoptosis and cellular stress responses. Activating p53 using the small molecule inhibitor NVP-CGM097 resulted in significant inhibition of VEEV infectious titers. Secondly, we investigated the therapeutic …
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In Vivo Significance of The Mdm4 and P73 Interaction During Development and Tumorigenesis
<p>The tumor suppressor protein p53 is negatively regulated by Mdm4 protein. The significance of such regulation was determined from mouse models. Mdm4-deficient mice are embryonic lethal at E7.5 in a <em>p53</em>-dependent manner. p73, a member of the p53-family, is a transcription factor with …
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53BP1 and double-strand break repair pathway choice in cancer
… of two competing repair pathways. The Tumour Protein P53 Binding Protein 1 (TP53BP1) gene product plays a central role in DSB repair pathway choice, promoting non-homologous end-joining (NHEJ) and counteracting homologous recombination (HR). As with all DNA damage response (DDR) proteins, …
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Characterisation of the role of ING5
The tumour suppressor protein p53 stops cell division by activating the expression of p21WAF1/CIP1, whenever it senses that a cells DNA is damaged. Thus, giving the cell a chance to repair the damaged DNA before its errors are duplicated and passed onto daughter cells. When p53 is mutated, it loses …
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