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Showing 1 to 7 of 7 for “"Protein libraries"”.
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Generation And Statistical Modeling Of Active Protein Chimeras: A Sequence Based Approach
<p>Generation of active protein chimeras is a valuable tool to probe the functional space of proteins. Statistical modeling is the next logical step, allowing us to build a model of gene fragment replaceability between species. In this thesis I begin to develop the statistical tools that are needed …
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Cell Targeted Ribosome Inactivating Proteins Derived from Protein Combinatorial Libraries
Combinatorial protein libraries based on a protein template offer a vast potential for deriving protein variants harboring new receptor specificity while retaining other tem-plate functions to serve as library search-engines, cell-routing sequences and therapeutic domains. This concept was tested …
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Development of the MAX randomisation technique
During the last decade the use of randomised gene libraries has had an enormous impact in the field of protein engineering. Such libraries comprise many variations of a single gene in which codon replacements are used to substitute key residues of the encoded protein. The expression of such …
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Incorporation and analysis of bilayer constituents in nanodiscs
… a lipid bilayer and two surrounding amphipathic proteins, have been utilized to house and solubilize integral membrane proteins and other molecules with large hydrophobic groups which limit their aqueous solubility. In the experiments described herein, lipids from the Nanodiscs’ bilayer, …
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Novel methods in computational analysis and design of protein-protein interactions : applications to phosphoregulated interactions
… methods for the analysis and design of protein-protein complexes, and their application to the study of the interactions of phosphopeptides with phosphopeptide-binding domain interactions. A novel protein-protein interaction type, the action-at-a-distance interaction, is described in the …
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DESIGNING A PLATFORM FOR DEVELOPING NOVEL GLYCAN-BINDING PROTEINS USING YEAST SURFACE DISPLAY
… reliable reagents. Many existing glycan-binding proteins exhibit broad specificity, low affinity, and are costly to produce. Thus, there is an urgent need for a rapid, high-throughput platform that can produce high-affinity glycan-binding proteins. We discuss the design of one such platform based …
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Analysis of membrane systems using lipoprotein nanodiscs
… However, the amphipathic nature of membrane proteins and lipids makes their analysis challenging. Engineered nanoscale lipoprotein Nanodiscs provide a well-defined and native-like lipid bilayer to solubilize membrane proteins and lipids. This dissertation details the analysis of Nanodiscs …