Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 217 for “"Protein kinase c"”.
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Functional Aspects of Protein Kinase C FRET Probe Performance
… influencing the performance of two FRET based protein kinase C (PKC) probes, KCP-1 and KCP-2. KCP-1 consists of the truncated PKC substrate pleckstrin, sandwiched between two fluorescent proteins, EYFP and GFP2. KCP-2 is a shortened version of KCP-1, missing the last 18 amino acids of the …
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The Effects of Atypical Protein Kinase C on TGFβ Signalling
… In this thesis, I examined the role of Protein Kinase C (PKC) in the regulation of TGFβ signalling pathways and found that the Atypical PKC isoforms (aPKC; a subgroup of the PKC family) indeed can alter TGFβ receptor signaling. My work has shown that the modulation of aPKC expression or …
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Localisation of Protein Kinase C in Apoptosis and Neurite Outgrowth
Protein kinase C (PKC) is a family of serine/threonine kinases, which are subgrouped into classical (a, bI, bII, g), novel (d, e, h, q) and atypical (z, i/l) isoforms. One major aim of this thesis work was to investigate if altered levels of PKC isoforms influence the apoptotic responses of …
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Aberrant Communication and Protein Kinase C in Experimental Autoimmune Thyroiditis
… aberrant communication in EAT. By studying protein kinases, we found pKC activity was increased in diseased thyrocytes with enhanced expression of several pKC isozymes and induction of two disease-specific pKC isozymes. The increase in pKC activity was not due to elevated enzymatic activity, …
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Roles of protein kinase C in cell death and breast cancer
Aberrant protein kinase C (PKC) activity and expression is implicated in different malignancies. To study the role of different PKC isoforms in breast cancer the expression of PCKα, δ and ε was evaluated in breast cancer tumors. In addition the effect of siRNA-mediated knockdown of the isoforms was …
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Formation of membrane domains during the activation of protein kinase C
The relationship between lipid domains and protein kinase C activity was studied via the direct visualization and quantitation of domains by fluorescence digital imaging microscopy. The lateral organization of protein kinase C, its substrate (the MARCKS peptide) and its three activators …
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Toward the design, synthesis and evaluation of Protein Kinase C inhibitors
Protein Kinase C (PKC) represents an important regulatory element in the signal transduction pathways of mammalian cells. Research interest has increased enormously since the discovery that PKC plays critical roles in cell differentiation, tumor promotion, oncogenesis and cell regulatory processes. …
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Role of protein kinase C isoforms in human breast tumor cell survival
Protein kinase C (PKC) is a family of serine/threonine kinases composed of 12 unique isoforms that function in a variety of cellular processes including proliferation, apoptosis, differentiation and carcinogenesis. The contribution of individual PKC isoforms to these processes is largely …
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Role of Protein Kinase C Zeta (PKCζ) in Regulation of Epithelial Tight Junctions
<p>Occludin is hyperphosphorylated on Ser and Thr residues in intact epithelial tight junctions. The dynamics of epithelial tight junctions appear to involve reversible phosphorylation of occludin on Ser and Thr residues. In the present study we determined the role of PKCζ in occludin …
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Molecular Mechanisms Underlying Differential Regulation of Platelet Dense Granule Secretion by Protein Kinase C delta
Protein Kinase C delta (PKCδ) is expressed in platelets and activated downstream of protease-activated receptors (PAR)s and glycoprotein VI (GPVI) receptors. We evaluated the role of PKCδ in platelets using two approaches - pharmacological and molecular genetic approach. In human platelets …
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Targeting Myeloid Protein Kinase C Signaling to Overcome Immune Suppression and Improve Immunotherapy in Cancer
… we first investigated the effect of protein kinase C (PKC) agonist PEP005 on MDSC expansion, differentiation, and recruitment to the tumor microenvironment. We found that PKC agonists decreased MDSC expansion from the bone marrow and induced MDSC differentiation to an APC-like …
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Protein kinase C modulates mitogenic signalling by epidermal growth factor in T51B rat liver cells.
… the epidermal growth factor (EGF) and the protein kinase C (PKC) signal-transduction pathways. T51B cells can be made proliferatively quiescent upon prolonged serum deprivation, which results in their growth-arrest at the G1 phase of the cell cycle. Addition of EGF (1.5$\eta$M) or, …
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Protein Kinase C as an Apoptosis Regulator and a Potential Prognostic Factor in Breast Cancer
Several protein kinase C (PKC) isoforms have been suggested as potential targets for breast cancer therapy. To explore the role for PKC isoforms in processes that facilitates malignant progression and the utility of PKC isoforms as biomarkers in breast cancer, we designed a study where we evaluated …
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Molecular mechanisms underlying morphological effects of protein kinase C under normal conditions and cellular stress.
The protein kinase C (PKC) family of serine/threonine kinases consists of 10-15 members. The PKC isoforms have central roles in many essential cellular processes, including proliferation, apoptosis, differentiation, cytoskeletal changes, and migration. The PKC family can be subgrouped into …
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Intracellular Calcium and Protein Kinase C Regulate Nonspecific Cell-Mediated Cytotoxicity by Mouse Lymphocytes (Calmodulin)
… nonspecific cytotoxicity, suggesting a role for protein kinase C in cytotoxicity. TPA and A23187 caused a synergistic increase in cytotoxicity when added together. A23187 also enhanced the cytotoxic activity of lymphocytes from nude and beige mouse spleens. Spleen cell populations in which Qa …
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MacMARCKS and the Protein Kinase C Signal Transduction Pathway: Role in Neural Secretion and Neural Development
<p>MacMARCKS is a second member of the MARCKS family of PKC substrates which integrate signal transduction events with changes to the actin cytoskeleton. Work presented here demonstrates diverse roles for MacMARCKS ranging from neural secretion to neural development. Chapters 3 and 4 describe the …
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