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Showing 1 to 20 of 32 for “"Protein extraction"”.
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Protein extraction using reverse micelles
… reverse micelles is of considerable advantage in protein extraction. A lot of researches have been done with ionic reverse micelles applied in protein extraction. However, this ability of non-ionic reverse micelles has not been fully understood and therefore requires more research. In this …
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The Recovery of Protein from Egg Yolk Protein Extraction Granule Byproduct
… its phosvitin and phospholipids. Granules are protein aggregates with complexes of phosvitin and high density lipoproteins linked by phosphocalcic bridges. In their native form, the proteins are mostly insoluble, however previous studies have shown the links can be broken by alterations in pH, …
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Technoeconomic Analysis of Protein Extraction from Ethanol Defatted Cold Press Canola Meal via Dry Fractionation
… industry has been spurred to supplant animal proteins in food formulations with underutilized plant protein sources and their coproducts due to concerns about sustainability and nutrition security. This research undertakes technoeconomic analysis (TEA) of protein extraction from ethanol …
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Synthesis and Polymerization of Substituted Stilbenes with Maleic Anhydride for Membrane Solubilization and Protein Extraction
Integral membrane proteins represent nearly 25% of protein encoding genes yet account for less than 2 percent of solved structures in protein databases. This underrepresentation is due to the difficulty in membrane protein isolation with the use of detergents. The difficulty in structural …
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A methodological investigation into the roots of the resurrection plant, Xerophyta viscosa, for further proteomic analyses
… subjected to various proteomic techniques. Three protein extraction methods were investigated, of which one method, was the most suited in isolating total protein from the root tissues of X. viscosa.
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Mouse Protection Assay For Measurement of Antibody and M Protein Antigen of Streptococcus Equi
… stimulated by vaccination with S. equi. M protein preparations. This passive mouse protection assay was then used in an opsonization inhibition assay developed to measure the M protein antigen of S. equi. Various antigen preparations were tested using this assay to study the effectiveness …
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Protein from pasture
… of the effects of regrowth period and season on protein extraction, separation and recovery. Herbage was harvested, adjusted to 10% tlry matter, pulped and (belt) pressed. Protein in the expressed juice was thermally coagulated and centrifugally separated to produce wet leaf protein concentrate …
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Numerical Simulation of Liquid-Solid Circulating Fluidized Beds
… are obtaining extensive attraction in the extraction process of functional proteins from industrial broth. A typical LSCFB is comprised of a riser, a downcomer, a liquid-solid separator, a top solids-return pipe and a bottom solids-return pipe. In light of the literature review conducted in …
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Effects of Ultrasonication on the Antioxidant and Anti-diabetic Properties of Hydrolyzed Oat Proteins
Ultrasonic treatments can be used to enhance protein extraction yields from foods. The objective of this work was to determine how the treatment of oat brans with ultrasounds affects the functionality and the physical properties of the hydrolyzed proteins. Oat brans were treated with ultrasonic …
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Isozyme patterns of selected isolates of Pholiota in the stirps "Adiposa"
… and mutual mating compatibility. Crude protein extracts of soluble proteins from mycelium grown in liquid shake culture were used to obtain general protein patterns and zyrnograms of esterases, (alphanaphthylamidases), phenoloxidases (laccases and tyrosinases), and peroxidases on …
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The extraction of Cytochrome C and DsRed2 into reverse micelles
… reverse micelles by the contacting of an aqueous protein-containing phase with an organic phase. Two important properties that differentiate the extraction profiles of these proteins are pI and size. Cytochrome c is a relatively small, monomeric protein with a pI of 10.6. It was easily extracted …
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Production of Protein Concentrates from Brewer's Spent Grain via Wet Fractionation and Enzymatic Purification
… and is rich in nutrients including 15-30% protein, making it a potentially valuable protein source for human food. Current challenges of extracting protein from BSG include low yields and high manufacturing costs, but the rising trend of plant-based diets for environmental and health …
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Lipid Biosynthesis and Maturation Signals in Batrachochytrium Dendrobatidis In Vitro
… of conspecifics. In addition, the isolation of proteins responsible for host-invasion was attempted using SDS-PAGE. Results from maturation assays support that multiple secretory molecules work synergistically to quicken maturation of zoosporangia. Further isolation of the responsible agents …
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Identification of Volatile Compounds Contributing to the Aroma of Pennycress and Camelina Seeds and Protein Extracts
… uses in the food industry due to their high protein contents. Minimal knowledge is known about their inherent volatile compounds and how they change during processing to yield a food ingredient. One variety of both pennycress and camelina, bred by Dr. David Marks and affiliates at the …
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Gel-based proteomic comparison of two strains of Propionibacterium freudenreichii ssp. shermanii
… and detect inter-strain differential protein expression. The influence of a number of parameters on the quality of 2-D gels was also investigated. The pH 3-7 and pH 3-11 NL proteome maps obtained displayed 305 and 356 spots, respectively, and detected the differential expression of 60 …
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Characterisation of the hydantoin-hydrolysing activity of pseudomonas putida strain RUKM3s and development of biocatalyst for amino acid production
… activity, and to evaluate techniques of protein extraction, enzyme isolation, purification and characterisation. The NCAAH ofRUKM3s is a dimer of approximately 60 k:Da, .with two subunits of approximately 30 k:Da each. The hydantoinase · is approximately 210 kDa. Methods of enzyme …
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DEVELOPMENT OF LEGUME-DERIVED INGREDIENTS AND FOODS ENRICHED IN BIOACTIVE COMPOUNDS USING DIFFERENT STRATEGIES
… many unit operations in food processing, such as extraction, homogenization, filtration, dehydration, freezing, thawing, and sanitization. In the initial phase, the study focused on Andean lupin (Lupinus mutabilis): a screening was conducted across 33 ecotypes, plus 5 controls belonging to the …
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The isolation and characterization of heat shock protein Hsp12 in Lipomyces starkeyi
The stress response protein Hsp 12 is induced in S. cerevisiae cells upon exposure to salt stress, heat shock, ethanol, and upon entry to stationary phase (Mtwisha et aI., 1998). In this study, the occurrence of proteins related to Hsp12 was investigated in a number of yeasts (namely, Saccharomyces …
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Serum-free media development using black soldier fly protein isolate and hydrolysate for cultivated meat
The global demand for animal proteins is projected to rise by 14% by 2030, amplifying the environmental toll of conventional animal-based protein production. Cultivated meat technology can alleviate the growing demand for protein and address the environmental and ethical concerns associated with …
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Effects of various salt purity levels on lipid oxidation and sensory characteristics of ground turkey and pork
… on lipid oxidation, sensory characteristics, protein extractability, and bind strength of ground turkey and pork. This study was a completely randomized design with 5 treatment groups and 6 replications in 2 species. Ground, turkey and pork meat was formulated into one hundred and fifty gram …
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