NDLTD logo NDLTD Union Catalog and Global ETD Search

Global ETD Search

Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.

Results

Showing 1 to 11 of 11 for “"Protein expression system"”.

  1. Cloning and characterization of MET2 in Pichia pastoris

    Thor, Der2018

    … yeast, Pichia pastoris, has been used as a protein expression system to express over 500 heterologous proteins. P. pastoris provides many advantages over other organisms that have been utilized for this purpose. In this project, we developed a new host/selectable marker and auxotrophic …

    u-pacific Repository record

  2. Cloning and characterization of the Pichia Pastoris PMR1 gene

    Grove, Heather Lee2005

    <p>Pichia pastoris, a popular protein expression system, is limited in its ability to secrete heterologous proteins. The PMR1 gene, the disruption of which is known to improve the secretion of prochymosin, human prourokinase, and human tissue plasminogen activator in Saccharomyces cerevisiae, was …

    u-pacific Repository record

  3. Isopeptide and Ester Bond Ubiquitination Regulate Degradation of the Human Dopamine Receptor 4

    Peeler, Jennifer C

    … 4 (hD4R) is a seven-transmembrane helical G protein-coupled receptor (GPCR) found in neural synaptic membranes. The neurotransmitter dopamine binds to and activates the hD4R, which is involved in central nervous system pathways that modulate cognition and circadian rhythms. The hD4R is the …

    rockefeller Repository record

  4. Baculovirus stability in serum-free lyophilized and wet storage conditions

    Colandro, Michelle ElizabethVirginia Tech2013

    The baculovirus expression vector system (BEVS) is an effective way to produce recombinant proteins for biopharmaceuticals. However baculovirus stocks are stored in subzero temperatures to maintain virus stability, and fetal bovine serum is commonly used in the storage solution. In an effort to …

    vt Repository record

  5. Development of a high throughput reporter system using β-Galactosidase in the yeast : Pichia Pastoris

    Nguyen, Jack2005

    … for its capabili ties in heterologous protein expression. In contrast to other host organisms such as bacteria or mammalian cells, P. pastoris offers many advantages over its counterparts. For example, P. pastoris is cost-effective in that it can grow to high cell densities on simple …

    u-pacific Repository record

  6. Development of low-temperature protein production systems by using cold-adapted bacteria, Shewanella livingstonensis Ac10 and Pseudoalteromonas nigrifaciens Sq02

    Kawai, SoichiroKyoto University2020

    kyoto Repository record

  7. Engineering a Reverse C4 Photosynthetic Pathway in C3 species Arabidopsis thaliana

    Di, ZhengaoUniversity of Cambridge2022

    … transition. Herein, a reverse C4 photosynthesis system that aimed to install C4 biochemistry in the opposite cell types from those used by C4 species was investigated. This approach was hypothesised to replicate the spatial patterning of the C4 biochemical pathway but make use of the native leaf …

    cambridge Repository record

  8. Engineering the Nicotiana benthamiana secretory pathway for the production of Lujo virus vaccines and diagnostic tools

    Isaacs, AbdulDepartment of Molecular and Cell Biology2023

    … in HEK293T cells and N. benthamiana for the protein LUJV GP-C∆TM (Lujo virus glycoprotein precursor without the transmembrane region). The LUJV GP-C∆TM was constructed from gene sequences of the envelope glycoprotein of Lujo virus and then adapted to production in HEK293T cells using the DNA …

    cape-town Repository record

  9. Reconstructing the gamma-secretase complex using the MultiBac baculovirus system for expression of heterologous multi-protein complexes

    Krishnaswamy, SudarsanEdith Cowan University, Research Online, Perth, Western Australia2010

    … larger parent molecule, the amyloid precursor protein (APP). The final stage in this process, which releases the Aβ peptide, is the cleavage of the APP-Cterminal fragment (APP-C99/C100) by a multi-subunit enzyme complex is referred to as γ-secretase. The enzyme‟s ability to cleave a number of …

    edithcowan Repository record

  10. The Effect of Select Biological and Environmental Factors on the Horizontal Gene Transfer and Functionality of the TOL Plasmid: A Case Study for Genetic Bioaugmentation

    Ikuma, Kaoru2011

    … structure and a long-term instability in ecosystem integrity. Furthermore, the degradative activities of such foreign microbes may be compromised when introduced into an unfamiliar environment during bioaugmentation, thereby resulting in a lower bioremediation potential. </p><p>Horizontal …

    duke Repository record

  11. Elucidation of the Molecular Mechanisms Underlying Estrogen-Mediated Estrogen Receptor Activation

    Coons, Laurel Aubrie2017

    … to turn genes on and off (i.e., the operating system). As in any other language, decoding the instructions between DNA and gene expression is the key for understanding transcriptional regulation. Without understanding the grammar of transcriptional regulation, we cannot tell which sequence …

    duke Repository record