Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 44 for “"Protein detection"”.
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Liquid Crystal Mediated Bioassay for Protein Detection
… further developed an immunoassay in which probe proteins are immobilized on the surface of liquid crystal (LC) droplets rather than on solid surfaces. The advantage of such a immunoassay is that the antibodies binding to the probe proteins can be transduced by the LC droplets directly. For …
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Microfluidic Techniques for Protein Detection and Manipulation
Protein-protein interactions are of central importance in biological systems. The interplay between proteins determines the functioning of such systems. Accordingly, a variety of methods have been established for the study of protein-protein interactions, but each class of methods has key …
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Aptamer-based nano-scale dielectric sensor for protein detection
The specific detection and precise quantification of protein molecules play an essential role in basic discovery research as well as in clinical practice. In this research work, a novel protein detection mechanism based on nanoscale dielectric sensor functionalized with aptamer probes is developed. …
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Advances in biophysical methods for protein detection and characterisation
Proteins are the building blocs of life and mediate nearly every function in the cell. They are therefore a major and incredibly wide research topic. Their functions and malfunctions have serious impacts on a wide range of diseases. Proteins may be used as extremely versatile tools for biology such …
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Carbon nanotube-based sensors for label-free protein detection
… highly-sensitive, selective, and real-time detection of biological analytes. However, important questions regarding sensor fabrication and reproducibility must be addressed for these sensors to be of practical value. Herein we describe the use of highly-purified, single-chirality SWNT which …
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Photonic-plasmonic coupling enhanced fluorescence for digital-resolution protein detection
Submission published under a 24 month embargo labeled 'Closed Access', the embargo will last until 2024-12-01
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Label-free carbon nanotube sensors for glycan and protein detection
… the long-held goal of accurate and rapid glycoprotein profiling without labeling or glycan liberation steps. Current methods of profiling oligosaccharides displayed on protein surfaces, such as liquid chromatography, mass spectrometry, capillary electrophoresis, and microarray methods, are …
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Sequence and Length Dependence of the Conductivity of Individual DNA Duplexes and Applications in Protein Detection
… charge transport (CT) and its application to protein detection. This chapter also highlights the advantages of using our method to study DNA-medicated CT, and illustrates its potential in biosensing. Chapter 2 includes the details of the fabrication procedures of the single molecule DNA …
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Digital Protein Analysis: Technologies for Protein Diagnostics and Proteomics through Single-Molecule Detection
Protein quantification tools are valuable in biological and biomedical research and in clinical applications because they enable measurements that elucidate physiological states, including disease states. Protein biomarker detection is likely to play a major role in patient health and aid in the …
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Particle-enhanced biomolecule detection using silicon photonic microring resonators
Protein detection is of great importance in both research and clinical settings. The use of proteins as diagnostic markers of disease is a driving force behind the development of advanced protein detection techniques. Of particular interest is the development of a detection platform which is both …
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Simulation and modelling to develop a nanopore-based device for ricin detection
Nanopore-based protein detection represents an exciting opportunity to develop ultrasensitive diagnostic devices that are both portable and affordable. Aptamer-encoded nanopores detect target molecules by binding to them to facilitate a corresponding blockage of ionic flow through the pore. …
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Silica Nanoparticle-based Novel Fluorescent Biosensors with Conjugated Polyelectrolyte-sensitized Signals
… of sensitive, low-cost and fast biomolecular detection is a key issue for clinical diagnosis of genetic and pathogenic diseases. This thesis presents the research that is focused on the design and fabrication of silica nanoparticle (NP) based fluorescent biosensors with the aid of conjugated …
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Development of Universal Biosensing Platforms Based on CRISPR/Cas12a systems
… and cost-effective method for diverse target detections. This thesis focusses on the development of CRISP/Cas12a based biosensing platforms for nucleic acid and protein detection. Two distinct CRISPR/Cas based diagnostic methods were developed. The first developed method is a plasmonic CRISPR …
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Silicon based photonic crystal coupled to an optical cavity for highly sensitive biodetection
… Frederick William Herschel in 1845, fluorescence detection technology has developed extensively to be used in disease diagnosis, DNA sequencing, cellular imaging, flow cytometry, forensics, and monitoring processes in biological systems. In order to achieve better sensitivity and higher …
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The Development of an Aptamer-based Surface Plasmon Resonance (SPR) Sensor for the Real-time Detection of Glycated Protein
The direct detection of blood proteins can benefit a number of scientific and clinical applications, one of which is glycated protein monitoring for diabetes diagnosis and treatment. The formation of glycated proteins is proportional to the average glucose concentration over a certain past time …
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Development and characterization of nanocarbon-based devices for sensing applications
… applications is reported and the tests on protein detection are shown. In the second part the development of Graphene oxide (GO)-based devices is shown and the electrical behavior of the devices is investigated in different ambient conditions and for different oxygen functionalization (GO …
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Hydrogel-based microfluidic assays for multiplexed medical diagnostics
… is high demand for next-generation biomolecule detection platforms arising from clinical need for more robust point-of-care diagnosis systems, fundamental research need to characterize complex biological processes, and the emergence of personalized medicine. These detection systems should …
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DNA Walkers: Emerging Analytical Applications, Biomolecular-Nanomaterial Probes and Biomolecule Sensors
… that range from nucleic acid sensing, to protein detection and to cellular imaging and analysis. The research described herein focuses on improving the understanding of biophysical processes involved in the design and operation of DNA walkers. Specifically, we developed a series of …
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A micromechanical biosensor with inherently differential readout
… enables sensitive, scalable and label-free detection of biomolecules in real-time. However, micromachined cantilevers are extremely sensitive to nonspecific chemical effects and temperature changes. This thesis explores a micromechanical sensor that suppresses disturbances by generating an …
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