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Showing 1 to 20 of 21 for “"Protein crystallization"”.
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Microfluidic platforms for membrane protein crystallization and in situ crystallography
Membrane proteins, biological macromolecules that reside in cellular membranes, play critical roles in many biological process, including signaling, transport, and intercellular communication. The malfunction of membrane proteins has been linked to the initiation or progression of many diseases …
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Microfluidic platforms for protein crystallization and in situ X-ray analysis
"Recent efforts associated with the Protein Structure Initiative from the National Institutes of Health (NIH) have eased the bottlenecks associated with protein expression, purification, and crystallization for structural analysis. While strategies have been developed for dealing with each of these …
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Microfluidic platforms for the characterization of in meso membrane protein crystallization
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Mesophase-based approaches for on-chip membrane protein crystallization and structure determination
Transmembrane proteins traverse the lipid bilayers of cell membranes and play a highly important role in many processes in vivo. Malfunctions of membrane proteins have been shown to cause a variety of disease states, for example, cystic fibrosis, hereditary hearing loss, and hypothyroidism. …
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An Investigation of Protein Interactions and the Relationship to Phase Behavior
Protein interactions in solution are inherently linked to phase behavior. A fundamental understanding of the relationship is necessary to develop efficient methods for crystallizing proteins. In this investigation, lysozyme second virial coefficient measurements consistently reflect trends in …
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Characterization of Protein Interactions in Eye Lens Crystallin Proteins and Crystallization
… theme of this research is to understand how protein interactions alter the state of aggregation and the characterization of these interactions through light scattering properties of dense suspensions. One context where protein interactions have an impact on the state of aggregation is in …
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The role of N-linked glycosylation on the structure and function of somatic angiotensin-converting enzyme
… and stability of ACE, but it interferes with protein crystallization. Previously, the N-glycan site occupancy required for the expression and stability of the individual domains of ACE was determined using minimally glycosylated (MG) N- and C-domain isoforms. However the role of glycosylation …
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Elucidation of Protein -Precipitant Phase Diagrams and Their Link to Crystal Quality
… solubility boundary, metastable zone width) of proteins of unknown structure using a very small sample volume. The knowledge of phase diagram of a protein/precipitant system thus obtained will be useful in obtaining high quality crystals for X-ray diffraction studies. Moreover, we use theory to …
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Model-based Design and Control of Biopharmaceutical Manufacturing Processes
… macroscopic bioreactor model for recombinant protein producing Pichia pastoris in defined medium, which enables the comprehensive understanding of cellular metabolism and optimization of bioreactor operations. For the downstream process, the thesis designs and implements laboratory unit …
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A computational investigation of nucleation processes in organic crystals
… in the atmosphere, the casting of metals, protein crystallization, biomineralization, the production of porous materials, and separation of pharmaceutical compounds from solution are a few examples of relevant nucleation processes. One pathway for nucleation to occur is homogeneous …
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Machine Learning-aided High-throughput Synthesis and Development of Optoelectronic Materials
… perovskite single crystals. I demonstrate that a Protein Crystallization Robot can be modified to prepare autonomously 288 independent perovskite single crystal growths. The robot acquires iii images as the crystal growth proceeds, allowing for direct measurement and classification of each …
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On Protein Crystal Nucleation Precursors
Protein crystallization is central to our understanding of molecular structures in biology, vital to industrial processes in the pharmaceutical industry, and increasingly appreciated as a critical block in understanding numerous disease pathologies. Although the nucleation process is critical in …
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The Development of Novel Apurinic/Aprymidinic Endonuclease/Redox-factor 1 Inhibitors for the Treatment of Human Melanoma
… First, <em>N</em>-terminally truncated APE/Ref-1 protein lacking the first 40 amino acid residues (∆40APE-1<sup>wt</sup>) was cloned into the pGEX-6P1 vector to express the GST-∆40APE-1<sup>wt</sup>protein. After cleavage of GST-tag, the concentrated ∆40APE-1<sup>wt</sup> protein was subjected to …
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X-ray compatible microfluidic platforms for studying protein structure and function
… of the three dimensional structure of a protein provides insight into its mechanism, as well as into potential sites for drug targeting. Often these insights provide functional information about an unknown protein, lead to a deeper understanding of how damaged proteins cause disease, and …
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Isothermal Titration Calorimetry Studies Of Protein-Mediated Interactions And Preliminary Structural Studies Of Tandem Pdz1-2 Domain Of Psd-95 Protein
<p>Protein-mediated interactions that involve multiple ligands in their binding mechanisms are critical for many cellular functions. The primary focus of this dissertation research was to investigate such interactions for two proteins, the PDZ domain and frataxin, involving peptide and metal …
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Structural and functional studies of the Interleukin-5 receptor system
… system (E.coli). After successful refolding proteins were purified to 95-99% Stable and soluble receptor:ligand complex was prepared. Each established purification and refolding procedures were subjected to optimization targeting maximal yields and purity. Produced receptor:ligand complex was …
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Structural study of lipid bicontinuous cubic phases for cellular delivery
… bicontinuous cubic phases have been utilized for protein crystallization and sensing applications. Lipid bicontinuous cubic phases also have a fusogenic property due to its resemblance of the fused structure. A simple argument by Helfrich on membrane elasticity and thermodynamics can rationalize …
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The self-assembly of proteins: Probing anisotropic protein-protein interactions using phase diagrams
… However for biological macromolecules such as proteins, anisotropic interaction energies and multiple degrees of freedom dominate the behaviour, making predictability of solution behaviour and crystallization extremely complex. To probe the impact of anisotropic interactions on the …
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