Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 33 for “"Protein bands"”.
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Investigation of the effects of antifolates on Escherichia coli 1810.
… membrane and peptidoglycan (OM-PG) associated proteins in the test bacteria. The test organisms were the sensitive Escherichia coli NCTC K-12 (E.coli K-12) and Escherichia coli 1810 (E.coli 1810) which was highly resistant to TMP. The checkerboard determinations indicated synergism with SD:TMP …
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The effects of weaning stress on the serum protein profile of calves : a proteomic analysis
… will cause sufficient stress in cattle to alter protein profiles in serum, which can be used to identify this type of stress. To do this we employed proteomic methodologies including two-dimensional gel electrophoresis and mass spectrometry to compare an abrupt weaned group of calves to a never …
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Steroid Stimulated Protein Synthesis in Fish Cell Cultures
Protein synthesis in Gonad primary cell cultures and Chinook Salmon Embryo cell cultures (CHSE-214) was stimulated by 17-beta-estradiol, 17-alpha-hydroxyprogesterone, and testosterone. Cells were treated for six hours with a 10-6 M concentration of steroid and radiolabelled with 35s-methionine for …
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A study of bovine salivary proteins
… approach to the study of the bovine salivary proteins was designed to: (a) Test the hypothesis that the salivary proteins, other than mucoprotein, might be related to, or involved in determining an animal’s susceptibility to bloat. (b) Gain some knowledge on the nature of the proteins in …
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Junction Specificity and Transmembrane Linkages of Integrins
… tissue yielded a complex of three prominent proteins with mobilities of 36, 30 and 18 kD. Amino acid sequencing of the purified proteins identified the 36 kD protein as GAPDH. The other 2 protein bands were heterogeneous, containing proteins found in the synaptic vesicle fusion core complex. …
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Molecular and Immunohistochemical Identification of a Sodium Hydrogen Exchanger-2C (Nhe2C) Paralog in the Gills of Marine Longhorn Sculpin (Myoxocephalus Octodecemspinosus)
Sodium hydrogen exchanger proteins (NHEs) are members of the cation proton antiporter superfamily (CPA) and are thought to function in fish for maintaining physiological ion concentrations and acid-base balances by excreting excess H+ ions from the body in exchange for Na+ ions. There are many more …
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Characterisation of marama bean protein
… Southern Africa. It is a very good source of protein, similar to soya beans and peanuts. The marama plant is very hardy and drought-tolerant. Therefore, it has great potential as an alternative to these other oilseed legumes. To facilitate the use of marama protein as a functional ingredient …
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Characterization of Protein Complexes and Protein Interaction Networks by Mass Spectrometry
… experimental proteomics approach for deciphering protein complexes and protein interaction networks in the budding and fission yeasts. Key steps of the employed analytical routine, including the purification of complexes and mass spectrometric identification of their subunits, were investigated in …
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Identification and Analysis of Peanut and Soybean Cross Reacting Allergens in Korean Children Sensitive to Peanut
… similarity in many parts of their seed storage proteins. the clinical manifestations of allergy to peanut and soybean are generally quiet distinct. And also, there are many cases of allergic patients with high level of soybean-IgE without clinical soybean allergy, especially in patients with …
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Antigenic and immunological determinants of acute allergic susceptibility to meat in a uniquely defined cohort in the Eastern Cape
… Raised IgE can be driven by exposure to parasite proteins and lipids with complex glycosylation patterns. Our study demonstrates the presence of alpha-gal in both adult and larval developmental stages of A. lumbricoides, Amblyomma hebraeum and Rhipicephalus evertsi. Alpha-gal glycosylation was …
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Effect of phospholipase C papain on feathering defect in 11% homogenized UHT processed cream
… in feathering test, phospholipid proportion, and protein patterns of surface material of homogenized fat globules. Creams that did not feather were stable pH at in acetate buffer as low as 5.00; creams that did exhibit feathering defect were stable in acetate buffer at pH of 5.45 and higher. The …
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Extracellular Polymeric Substances in Activated Sludge Flocs: Extraction, Identification, and Investigation of Their Link with Cations and Fate in Sludge Digestion
… gel electrophoresis (SDS-PAGE) and protein identification by liquid chromatography tandem mass spectrometry (LC/MS/MS), and hemaagglutination (HA)/HA inhibition assays; and 3) investigating the fate of EPS in sludge digestion using SDS-PAGE. Evaluation of prior research and data from …
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Patterns of protein expression in tissues of the killifish, Fundulus heteroclitus and Fundulus grandis
… stress. The thesis focuses on patterns of protein expression in Fundulus heteroclitus and F. grandis.The patterns of protein expression were investigated using traditional methods of enzyme activity measurements and recent proteomic approaches. The findings of the study can be used to guide …
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Development of a novel liquid chromatography based tool to study post-translational modifications
… modification and involves separation of modified proteins from non-modified ones. The drawback of using a modification specific method is that there is a lack of flexibility in its usage for other modifications. The goal of these studies was to investigate the possibility of obtaining a similar …
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APPLICATIONS OF DYNAMIC ISOELECTRIC/ANISOTROPY BINDING LIGAND ASSAY FOR PROTEOMIC RESEARCH
… this work reviews the need for high efficiency protein separation techniques. Dynamic isoelectric focusing (DIEF) is new technique similar to capillary isoelectric focusing (CIEF) invented by Dr. Luke Tolley at Southern Illinois University Carbondale. Using DIEF, the electric field inside the …
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Palladium uptake and its effect on behavioural and molecular markers in the freshwater shrimp Gammarus pulex
… acid reactive substances (TBARS); heat shock proteins (HSPs) and protein phosphorylation) endpoints. Pd caused a significant reduction in the survival of G. pulex with a 96 h LC50 of 0.52 mg/L (4.89 μM). Pt or Rh increased the survival of G. pulex exposed to Pd. However, this was not …
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Purification of A Serum Factor That Triggers Cell Cycle Re-entry In Differentiated Newt Myotubes
… the serum factor as a thermo-labile, pH- and proteinase K-sensitive, high molecular weight protein that is resistant to denaturing conditions such as SDS, urea or organic solvents. Surprisingly, under denaturing conditions the activity behaves as a low molecular weight protein that displays …
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Isolation and partial characterization of host cell wall surface glycoproteins: their involvement in agglutination, attachment and appressorium formation by piptocephalis virginiana
Cell surface proteins obtained by alkaline extraction from isolated cell walls of Mortierella pusilla and M. candelabrum, host and nonhost, respectively, to the mycoparasite, Piptocephalis virginiana, were tested for their ability to agglutinate mycoparasite spores. The host cell wall protein …
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Control of endometrial secretion in cattle and production of transgenic swine
… structure and progesterone (P₄) on endometrial protein secretion. Day 0 (estrus) endometrial tissue released more protein than tissue collected on d 9, 14 or 18. Protein synthesis was greater on d 0 and 18 than d 9 and 14. Endometrium from the uterine horn contralateral to the ovulatory …
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The isolation, identification and detection of Clostridium Difficile
… of strains of Cl.difficile. Although complex protein profiles were obtained, the method was found to be reproducible and produced ten different protein patterns from the isolates tested. There was no difference between the protein patterns of the isolates obtained from asymptomatic infants and …
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