Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 12 of 12 for “"Protein affinity"”.
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Identification and Function of Beta-1,4-Galactosyltransferase Associated Proteins
… testis phage display cDNA library screening and protein affinity chromatography were applied to identify GalTase-I associated proteins. Several proteins were identified as candidates that interact with GalTase-I and potentially play important roles during fertilization. Deciphering proteins that …
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DEVELOPMENT OF VIRTUAL SCREENING METHODOLOGIES FOR IDENTIFYING RNA-BASED THERAPIES
… of computational methodologies for predicting protein-RNA binding affinities and designing RNA sequences with specific functional outcomes. The research introduces PANTHER (Protein-Affinity for Nucleic Target-binding, Hybridization, and Energy Regression), a novel machine learning-based scoring …
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Biophysical characterization of high affinity engineered single chain Fv antibody fragments
High affinity antibody binding interactions are important for both pharmaceutical and biotechnological uses. However, designing higher affinity interactions has remained difficult. Both high affinity interactions from nature and the results from directed evolution affinity maturation processes may …
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Thin film integrated optical waveguides for biosensing using local evanescent field detection
… refractive index to be modified by attaching proteins or patterning organic films. This design also allows the evanescent field intensity to be measured using near field scanning optical microscopy or a silicon photo detector array. The fabrication and characterization of a waveguide device …
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Expression, Isolation and Purification of Human Zona Pellucida Protein 3
… zona pellucida is composed of three major glycoproteins. One of them, ZP3, participates in the primary sperm binding and in the subsequent triggering of the spermatozoa's acrosome reaction. Studies on the role of this specific protein in the human fertilization process are hampered by the …
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High relaxivity biomolecule based contrast agents engineered for molecular functional magnetic resonance imaging
… two approaches for developing high relaxivity protein-based contrast agents to address the issues of low detectability. First, we coupled monoamine sensing with the disaggregation of superparamagnetic iron oxide nanoparticles (SPIOs). Ligand detection was imparted by integration of a monoamine …
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Analyses of the MEC1 DNA Damage Pathway in Saccharomyces cerevisiae
… a functionally uncharacterized MEC1 DNA repair protein, HUG1, in the DNA damage response was also explored. Using overexpression phenotype and subcellular localization assays, we demonstrated that HUG1 is a negative regulator of the MEC1 pathway and that its co-localization with the positive …
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The production, harvest and adsorptive recovery of an infectious herpes simplex virus vaccine
… a reliable vaccine against herpes virus. Viral protein vaccines as yet have proved unsuccessful to meet the challenge of raising an appropriate immune response. Cantab Pharmaceuticals has produced a virus vaccine that can undergo one round of replication in the recipient in order to produce a …
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Paper-based Molecular Technologies for Faster, More Accessible Infectious Disease Diagnostics
… colorimetric diagnostic tests to detect protein and nucleic acid biomarkers of infectious disease in clinically relevant matrices. Protein affinity reagents, such as antibodies and engineered binder proteins, were integrated into paper devices, and antibody stabilization methods were …
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Development of tissue engineering strategies for supporting regeneration after injuries of the nervous system
… non-adhesive properties towards cells and proteins which enabled controlled investigation of the effects actuated by the fibres. Three different polymeric materials were developed and electrospun into fibres: Firstly, blends of poly(caprolactone) (PCL) and the extracellular matrix protein …
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Microfluidic diffusional sizing at the single molecule level
… much suited to measuring the size of individual proteins and higher-order protein assemblies, and MDS has indeed been applied to good effect to study proteins and the strength of their interactions. However, conven- tional MDS is inherently an ensemble level method - it is performed in the …
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A systematic study of ion-induced nanopatterning on photonic crystal-based label-free optical biosensor
… and chemistry are two main factors affecting protein affinity and conformation on biointerfaces, and the topography and chemistry can be tailored to improve their affinity and adhesion to the surface. It is also known that the presence of nanostructures can increase sensor sensitivity by …