Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 5 of 5 for “"Protein Thermal Stability"”.
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Thioaptamers Targeting Dengue Virus Type-2 Envelope Protein Domain III
… the host cell membrane using surface envelope protein (E). The E protein ectodomain contains three domains, EDI, EDII and EDIII. The highly immunogenic EDIII is the putative receptor binding domain that also binds neutralizing antibodies. Thus, EDIII is an attractive target for development of …
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Effects of macromolecular crowding and small ions on the folding, structure, and stability of Desulfovibrio desufuricans flavodoxin
The intracellular environment in which most proteins fold and function contains a range of biomolecules that results in significant volume exclusion, thus contrasting to the dilute buffer conditions common to most in vitro studies. In addition to intracellular macromolecular crowding, cells are …
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Analysis and Redesign of Protein-Protein Interactions: A Hotspot-Centric View
… discoveries from mutational analysis of protein interfaces is that often a large percentage of interface residues negligibly perturb the binding energy upon mutation, while residues in a few critical "hotspots" drastically reduce affinity when mutated. The organization of protein …
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Investigating the role of UDP-<i>N</i>-acetylhexosamine pyrophosphorylases in regulating <i>O</i>-GlcNAcylation in human disease
Modification of proteins on serine and threonine residues with O-linked N-acetyl-β-<sub>D</sub>glucosamine (O-GlcNAc) was first observed on the surface of lymphocytes in 1984. It was then found that over 1,000 nucleocytoplasmic proteins are modified by O-GlcNAc (O-GlcNAcylation). Interestingly, …
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Investigating Lipase Stability in Hydrophobic Solvent at Extreme Temperatures: A Molecular Dynamics Study of Candida Antarctica Lipase B in Polydimethylsiloxane
… (PDMS) as a reaction solvent on the structural stability and conformational dynamics of a well known enzyme, Candida Antarctica Lipase B (CALB). In a previous study, CALB was utilized as the catalyst for the polymerization reaction of disiloxane-containing polyesters. The results demonstrated a …