Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 24 for “"Protein Oxidation"”.
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Biochemical Investigations of Macular Degeneration: The Significance of Protein Oxidation including Novel Methods for Its Study
… to assist in AMD research. Specifically, the protein composition of retinal LF was assessed to elucidate its origin. These findings are reported in chapter 2. The accumulation, composition and phototoxicity of MLF were analyzed to assess MLF's origin and possible contribution to AMD. These …
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Oxidation of methionine residues in protein pharmaceuticals
… to the access to the solvent but do not affect oxidation in a specific manner, consistent with the good correlation between 2SWCN's and the rates of oxidation. A comprehensive picture of oxidation is thus developed. It allows an accurate prediction of protein oxidation, and provides a rationale …
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Effects of combined exposure to ethanol and ionizing radiation on the antioxidant status of in vitro and in vivo models
… exposure has been shown to induce lipid peroxidation, DNA damage, protein oxidation, and GSH depletion, as well. It was postulated that initial exposure to ethanol, followed by ionizing radiation, would result in heightened oxidative stress. The in vitro model used in this investigation was …
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NEW METHODS FOR DETECTING BIOMARKERS OF OXIDATIVE STRESS AND REDOX SIGNALING ON PROTEIN CYSTEINE RESIDUES
… redox-based signaling occurs is reversible oxidation of cysteine residues in the presence of low concentrations of these oxidants. S-Nitrosothiols (RSNO) and sulfenic acids (RSOH) are thought to be two of the most common cysteine modifications, and formation of these species reversibly …
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Oxidative Protein Folding Pathways In Gram-Positive Actinobacteria
… are important for the stability of many secreted proteins. These covalent linkages, which result from the oxidation of neighboring cysteine (Cys) residues, are often rate-limiting steps for protein folding and maturation. Disulfide bond formation is restricted to extracellular oxidizing …
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Oxidative stress biomarkers in dementia
… Moreover, the oxidative status of specific proteins was investigated using both hypothesis driven and proteomic approaches. Results presented in this thesis suggest that global plasma protein oxidation levels are not different when AD and control subjects are compared, but that individual …
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Contribution à l'étude du vieillissement physico-chimique de produits prégélatinisés obtenus par technologie roller et développement d'une méthode d'analyse de leurs volatils par TD-GC-MS
… the properties evolution was probably due to the protein oxidation and denaturation, and the starch depolymerization. Next, the influence of chemical (addition of food additives, E170 and E340ii), physical (reduction of particle size) and biological (choice of cereal, wheat) parameters on the …
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Circadian rhythms of redox state and regulation of neuronal excitability in suprachiasmatic nucleus of rodents
… cycles manifest as circadian rhythms in protein oxidation have been reported in anucleate cells, where no transcription occurs. Whether the brain clock expresses redox cycles and how such metabolic oscillations might affect neuronal physiology are unknown. Here we show a self-sustained …
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Computational modeling of the IL-4 pathway to understand principles of systemic redox regulation in cell signaling
… and our analyses suggest that reversible oxidation of tyrosine phosphatases is the primary mechanism of redox regulation in this pathway. Additional computational methods have been developed to study ROS as mediators of crosstalk between signaling pathways, to optimize model parameters, …
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Oyster Mushroom (Pleurotus ostreatus) Stipe Peptides as in-vitro Radical Scavenging, Ferrous Iron Chelating, & Ferric Reducing Antioxidant Compounds
… Additionally, the effects of peptides on protein carbonyl formation during myofibrillar oxidation is unknown. Oxidation of lysine, arginine, and proline in myofibrillar protein from red meat results in reduced meat quality owing to changes in water composition. Therefore, the objective of …
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The Effects of Neuroendocrine Stress on Larval Development
… hormones on metabolism, energetics, and nutrient oxidation in larval wood frogs. The results show that GC hormones increase metabolism, reduce lipid stores, and increase protein oxidation during metamorphic climax. Chapter three focuses on the effects of GC hormones on immune function and r …
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Characterization and Application of Peanut Root Extracts
Lipid oxidation is one of the leading causes of food quality degradation. Manufacturers typically add antioxidants or purge a product's package of oxygen to inhibit oxidation and the resulting off-flavors. Synthetic antioxidants (e.g. BHT, BHA) and some natural antioxidants (e.g. α-tocopherol) have …
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Proteomics in Nasal Lavage Fluids from Persulfate Challenged Hairdressers
… measure proteomic changes, both with respect to protein abundances and protein oxidation, in nasal lavage fluids (NLF) from hairdressers challenged with persulfates. Proteins regulate most biological processes in the human body. Changes in protein levels may therefore provide a better …
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Mass Spectrometric Characterization and Fluorophore-Assisted Light Inactivation of Human Excitatory Amino Acid Transporter
… and mechanism of this integral membrane protein is limited. The goal of this study was to use pharmacological, mass spectrometric (MS) and photochemical approaches to probe EAAT2. For MS characterization, a hexahis epitope was incorporated into the N-terminus of human EAAT2. The …
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Mass Spectrometric Characterization and Fluorophore-Assisted Light Inactivation of Human Excitatory Amino Acid Transporter
… and mechanism of this integral membrane protein is limited. The goal of this study was to use pharmacological, mass spectrometric (MS) and photochemical approaches to probe EAAT2. For MS characterization, a hexahis epitope was incorporated into the N-terminus of human EAAT2. The …
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Tooth wear in Irish teenagers: a laboratory and epidemiological study
… profiles were determined for subsets; the protein concentration was determined with Bradford protein assay and protein carbonyl concentration (a protein oxidation marker) was determined spectrophotometrically. Gel-electrophoresis and mass spectrometry determined proteins and ion …
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Evolutionary Implications and Genetic Basis of Peroxide Survival in Saccharomyces Cerevisiae
… of microbial invaders by inflicting DNA lesions, protein oxidation and lipid membrane modifications. Pathogens protect themselves with enzymes and scavenging proteins. This study investigated population genetic, biochemical and genetic aspects of peroxide survival in <i>Saccharomyces cerevisiae …
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Reactive oxygen and nitrogen species production in cardiomyoblasts during hypoxia and reoxygenation
… and cell death; associated with an increase in protein oxidation. After 4h, apoptosis induction under 2% O2 and 10% O2 was dependent on the production of mitochondrial superoxide (O2-•) and nitric oxide (•NO), partly from nitric oxide synthase (NOS). Both apoptotic and necrotic cell death during …
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Mechanisms Regulating the Response of Arabidopsis thaliana to Heat Stress
… of heat and drought stress revealed that multiprotein bridging factor 1c (MBF1c) is highly activated during the stress combination. In this study, to test the role of MBF1c in the response of plants to abiotic stresses, transgenic plants constitutively expressing MBF1c were generated and …
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The mechanism by which manganese protects Escherichia coli from hydrogen peroxide
… the substitution of manganese may prevent protein damage. Accordingly, during H2O2 stress, mutants that cannot import manganese and/or are unable to sequester iron suffer high rates of protein oxidation. To directly test our hypothesis, I studied three functionally distinct mononuclear …
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