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Showing 1 to 20 of 24 for “"Protein Molecule"”.
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Relaxation dynamics in heme proteins
A protein molecule possesses many conformational substates that are likely arranged in a hierarchy consisting of a number of tiers. A hierarchical organization of conformational substates is expected to give rise to a multitude of nonequilibrium relaxation phenomena. If the temperature is lowered, …
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Progress in the Synthesis and Modification of Glass Surfaces Necessary to the Study of Single Molecule Protein Folding and Nanolithography
… research was to monitor the folding of a single protein molecule using sp-FRET. In order to accomplish this task, surface modification of a glass coverslip was necessary to prevent non-specific binding. Modification of the surface was accomplished by derivatizing the surface with PEG's and …
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Information Content in Ribonuclease A: An Investigation of Protein Structure and Identification of New Qsars
… between statistical information expressed by protein structure and the resulting enzymatic function. All molecules contain information in their atom/covalent bond structure. It is this structural information that confers the physical and chemical properties of the molecule. It was the goal of …
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Relaxation dynamics in heme proteins
A protein molecule possesses many conformational substates that are likely arranged in . a hierarchy consisting of a number of tiers. A hierarchical organization of conformational substates is expected to give rise to a multitude of nonequilibrium relaxation phenomena. H the temperature is lowered, …
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Exploring the molecular architecture of proteins: Method developments in structure prediction and design
<p>Proteins are molecular machines of life in the truest sense. Being the expressors of genotype, proteins have been a focus in structural biology. Since the first characterization and structure determination of protein molecule more than half a century ago1, our understanding of protein structure …
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Isolation and Characterization of an Isoprenoid Transporter Protein in Manduca sexta
… proper cell function. Isoprenoids are metabolite molecules implicated in cell growth, energy production, and act as precursor to steroids, cholesterol, and other derived compounds in living organisms. Within the isoprenoid pathway, isopentenyl pyrophosphate (IPP) was found to be transported by a …
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Aptamer-based nano-scale dielectric sensor for protein detection
… specific detection and precise quantification of protein molecules play an essential role in basic discovery research as well as in clinical practice. In this research work, a novel protein detection mechanism based on nanoscale dielectric sensor functionalized with aptamer probes is developed. …
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An exploration of some aspects of molecular replacement in macromolecular crystallography
… An initial chapter describes the structure of a protein, the methods based on the use of X-rays and computer analysis of diffraction patterns to determine crystal structure, and the subsequent derivation of the structure of part or all of a protein molecule. Work to determine the structure of the …
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Studies of Protein-Protein and Protein-Water Interactions by Small Angle X-Ray Scattering, Terahertz Spectroscopy, ASMOS, And Computer Simulation
The protein folding problem has been one of the most challenging subjects in biological physics due to its complexity. Energy landscape theory based on statistical mechanics provides a thermodynamic interpretation of the protein folding process. We have been working to answer fundamental questions …
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Wechselwirkung von dünnen Schichten aus HVZ Polyestern im wässrigen Medium mit Modellproteinen
… sensitive techniques with respect to further protein adsorption investigations. In Chapter 3.3 the strategy for the fabrication of surface attached carboxyl terminated HBP using "grafting to" technique is developed. The Chapter 4 consists of two parts: The first (theoretic) part …
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Complex Proteoform Identification Using Top-Down Mass Spectrometry
Proteoforms are distinct protein molecule forms created by variations in genes, gene expression, and other biological processes. Many proteoforms contain multiple primary structural alterations, including amino acid substitutions, terminal truncations, and posttranslational modifications. These …
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Determination of Biomolecular Interdomain Motions using Nuclear Magnetic Resonance
<p>Biological macromolecules can rearrange interdomain orientations when binding to various partners. Interdomain dynamics serve as a molecular mechanism to guide the transitions between orientations. However, our understanding of interdomain dynamics is limited because a useful description of …
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DNA-Protein Interactions in f1 DNA Replication
… analyzes the interaction of two DNA-binding proteins with the plus strand replication origin of bacteriophage f1. The origin has a bipartite structure consisting of a required core origin region and an adjacent A+T- rich enhancer sequence that potentiates replication approximately 100-fold. …
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Semi-Synthesis of the Transcription Factor SMAD2 Containing Caging Groups and Phosphoaminoacid Analogues
<p>Post-translational modification (PTM) of a protein refers to any chemical change that occurs to the protein after its ribosomal synthesis. The seemingly endless number of PTMs can endow proteins with new functionalities that are not present in the unmodified proteins. In order to study the …
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Protein stability: Impact of formulation excipients and manufacturing processes in protein-based pharmaceuticals
Presently, over 300 proteins or peptide based therapeutic medicines have been approved by the FDA owing to advances in protein engineering and technology. However, majority of these protein-based medications are unstable or have limited shelf life when in aqueous form. During pre-formulation and …
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Aspects of the chemistry of milk protein films formed on stainless steel
… properties of milk and the surface chemistry of proteins was reviewed to gain an insight into the formation, of protein-mineral films (“milkstone”) on the contact surfaces of dairy milk lines. Protein adsorption processes were considered fundamental to the formation of such deposits. Standard …
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Characterization of SsoSSB, Sso1450, Sso2001 Proteins and Analysis of CRISPR and cas Genes from Sulfolobus solfataricus P2
… a newly discovered single-stranded binding (SSB) protein from Sso P2 and the computational and experimental analyses of clustered regularly interspaced short palindromic repeats (CRISPRs) and CRISPR-associated (cas) genes, respectively. The DNA-binding properties of SsoSSB, the organization of the …
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A Statistical-Mechanical Model for the Binding of Flexible Ligands to Proteins
… presented for the binding of flexible ligands to proteins. The model explicitly accounts for the ability of these chain-like molecules to bind in a large number of configurations (including many in which not all segments are in contact with the protein) and therefore is quite different from the …
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