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Showing 1 to 5 of 5 for “"Protein Induced Fluorescence Enhancement"”.
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Characterization of protein induced fluorescence enhancement (PIFE): A label-free protein assay with short distance sensitivity
… used for monitoring the interactions between a protein and a nucleic acid. It typically requires a site-specific labeling of the protein, which can be labor-intensive and inefficient. In addition, for common dye pairs, the distance sensitivity of FRET lies only between 3-7 nm. In this report, we …
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Unveiling Dna Polymerase Synthesis And Proofreading Activities - One Molecule At A Time
… Second, we applied a new single molecule fluorescence technique, single molecule protein induced fluorescence enhancement (smPIFE), to our system to accurately measure binding dynamics and to strengthen our interpretation of the polymerase position on the DNA. Third, determined how …
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Single molecule pull down of double strand RNA binding proteins
… as double-strand RNAs (dsRNAs). The class of proteins responsible for processing dsRNAs is termed double-stranded RNA binding proteins (dsRBP). In recent decades, an increasing number of reports have shown the role of dsRBP-dsRNA interaction as core strategy in various cellular regulation …
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Transfer rna dynamics and transfer-messenger rna accommodation in bacterial ribosomes at the single-molecule level
<p>Single-molecule spectroscopy, protein-induced fluorescence enhancement (PIFE), fluorescence resonance energy transfer (FRET), and several biochemical tools were applied to study transfer RNA (tRNA) dynamics and transfer-messenger RNA (tmRNA) accommodation inside ribosomes. In the first project …
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Single molecule investigations into DNA replication, recombination and repair
… to investigate the repair mechanisms at single protein level with nanometer resolution and millisecond time scale. The ability to fluorescently label different parts of the protein or protein complex allows us to monitor in real-time conformational changes as well as the mechanistic details with …