Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 39 for “"Protein G"”.
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Studies on the interaction of a single domain of protein G from streptococcus with human Fc and Fab
Protein G (SpG) is a Type III bacterial Fc receptor that binds selectively and non-antigenically to both the Fc and Fab regions of IgG. Here studies are described aimed at understanding the individual contributions to complex stability made by amino acid residues known to make contacts with either …
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Protein FOG at the interface between G streptococci and human host defence lines
… human defence lines are investigated. A novel M protein from GGS, denoted FOG, was isolated, recombinantly expressed, and purified. FOG-positive strains survive in human whole blood wheras a FOG-negative strain did not. Addition of soluble FOG, but not protein G, leads to restoration of survival …
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Detection of dementia (Alzheimer’s disease) marker tau using a four gold microband electrode impedance sensor
… biosensor system for the detection of tau protein, which is one of the most sensitive markers for the prediction of Alzheimer’s and the level of this marker in the plasma has been found to correlate with cerebro-spinal fluid (CSF) load. The biosensor is based on the formation of stable …
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Investigating pathogen 4Fe-4S protein targets and cancer chemotherapies with bisphosphonate/diphosphate inhibitors
… enzymes of the MEP pathway, IspG (iron sulfur protein G; also known as GcpE and E-4-hydroxy-2-C-methyl-erythritol pyrophosphate synthase) and IspH (also know as LytB and E-4-hydroxy-2-C-methyl-erythritol pyrophosphate reductase), respectively. Investigation of novel cancer drugs focuses on …
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Biomolecular Solid-State Nuclear Magnetic Resonance Methods for Spectral Assignment and High-Resolution Structure Determination of Proteins
… the elucidation of structure in microcrystalline proteins are presented, utilizing the streptococcal beta-1 immunoglobulin binding domain of protein G (GB1) as a model protein. Complete 13C and 15N chemical shifts are presented based on 2D 13C-13C, 15N-13C and 3D 15N-13C- 13C and 13C-15N-13C …
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Surface Directed Monoclonal Antibodies against STEC Aid in the Reduction of Pathogen Detection Times from Food and Water
… specific mAbs immobilized on covalently coupled protein G polystyrene beads and diluted enrichment media. A combination of these strategies together with immunofluorescence microscopy (IMS) and polymerase chain reaction (PCR) could provide diagnostic laboratories with a means to confirm a …
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Mechanistic studies on chemical instabilities of recombinant proteins
Protein molecules are being widely used as pharmaceuticals for treating diseases ranging from diabetes and haemophilia to various types of cancers due to their great potency and specificity. However, these macromolecules are intrinsically unstable in aqueous solutions, due to the existence of …
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STRUCTURE-BASED APPROACHES TO DATA-DRIVEN PROTEIN FOLDING, AGGREGATION, AND SELF-ASSEMBLY
Predicting protein dynamics on a molecular level is central to understanding and ultimately controlling the biomolecular machines that govern life. Despite advances in molecular dynamics and AI-based structure prediction, the accurate and efficient simulation of complex self-assembly processes - …
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THE ROLE OF BOTANICAL OILS ENRICHED IN FADS2-DERIVED N-3 VS. N-6 POLYUNSATURATED FATTY ACIDS IN PREVENTION OF ATHEROSCLEROSIS
… also investigated the role an anti-inflammatory protein G-protein coupled receptor 120(GPR120) that is activated by PUFAs in atheroprotection and hypothesized that dietary n-3 PUFAs would lead to greater activation of GPR120 and less inflammation than n-6 PUFAs.
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Solid-State Magic-Angle Spinning NMR Methods for Tensor Measurements and Protein Structure Refinement Using Chemical Shift Tensors
… beta-1 immunoglobulin binding domain of protein G (GB1). Experimental techniques presented include: three-dimensional experiments that recouple 13C or 15N chemical shift anisotropy (CSA) pseudostatic powder lineshapes, slow magic angle spinning (MAS) analysis of highly-13C,15N-enriched …
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Herstellung und Charakterisierung monoklonaler Antikörper gegen equine Leukozyten
… 4-13, 4-58, 1-19 and 6-39 have been purified by protein G-sepharoses chromatography and, except mAb 4-58, biotinylated. MAb 4-13, mAb 4-58 and mAb 5-50 are binding specifically to equine T-cells. Flow cytometer analyses demonstrated an increased frequency following the stimulation with …
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Role of Chaperonin CCT in G-protein Biosynthesis
… and function to assist the folding of other proteins. All chaperonins consist of two stacked rings, which come together to form a central cavity where folding can take place. The eukaryotic cytosolic chaperonin containing TCP1 (CCT) is a member of the group II chaperonins, which are defined …
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Identification of Anti-Beta2 Glycoprotein I Auto-antibody Regulated Gene Targets in the Primary Antiphospholipid Syndrome Using Gene Microarray Analysis
Anti-Beta2-Glycoprotein I antibodies (anti-2GPI) are strongly associated with thrombosis in patients with primary antiphospholipid syndrome (PAPS). Anti-2GPI activate endothelial cells (EC) resulting in a pro-thrombotic and pro-inflammatory phenotype. In order to characterise EC gene regulation …
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Design and Application of Hollow Silica Microspheres for Density-based Bioseparations
… Methods for surface functionalization with protein G were established with an estimated binding capacity of 31 µg/mg for size-fractionated microspheres 38 µm in diameter, and 50 µg/mg for size-fractionated microspheres 81 µm in diameter. We then applied the molecular buoy bioseparation …
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Design and application of hollow silica microspheres for density-based bioseparations
… Methods for surface functionalization with protein G were established with an estimated binding capacity of 31 µg/mg for size-fractionated microspheres 38 µm in diameter, and 50 µg/mg for size-fractionated microspheres 81 µm in diameter. We then applied the molecular buoy bioseparation …
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Sex differences in the cardiac beta-adrenergic receptor contractile response
… differences in the expression of G<sub>s</sub>á protein, G<sub>i-2</sub>á protein, PDE4D, PP1 or PP2A as determined with western blotting. Adenylyl cyclase V/VI was expressed in significantly higher levels in female myocytes. Females showed enhanced cAMP accumulation upon ISO treatment with and …
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Dissecting the Molecular Basis of the Antiphospholipid Syndrome
… and APS patients, respectively, and purified by protein G-sepharose chromatography. We found that aPL prevents VEGF-mediated attenuation of monocyte adhesion to cultured endothelial cells and this is reversed by an NO donor, indicating a role for eNOS antagonism. In contrast, NHIgG has no effect …
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Nutritional studies in ambulatory oncology patients: impact of malnutrition on survival, patient beliefs, debunking myths, and a novel targeted intervention to attenuate nutritional decline
… serving of the test product (306 kcals, 18g protein, 1.1g EPA, 0.7g DHA and 10µg vitamin D) for 8-9 weeks. Median energy (kcals) and protein (g) increased significantly from baseline to T2 (after 8/9 weeks). CT analysis of body composition revealed that despite the majority being overweight …
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Improving nitrogen efficiency and profitability of dairy cattle in the United States
… CP diets was the cost per unit of metabolizable protein (MP). In the second study, three treatments were fed to lactating Holstein cows (n = 48) to test proof of concept of NASEM 2021 and a nonlinear optimizer: a control balanced to fulfill all nutrient needs of lactating dairy cows producing 45 …
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Relationship of Body Attitude and Personality Characteristics to Dietary Intake in Female Collegiate Athletes
… bulimia, interpersonal distrust, dietary protein (g) intake, dietary fat (g) intake, dietary fiber (g) intake, and vitamin C (mg) intake. No significant differences were found between the lacrosse and dance team on dietary intake, body attitude or personality traits. Significant …
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