Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 203 for “"Protein Engineering"”.
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Protein engineering and pattern recognition
Thesis (M.S.)--Massachusetts Institute of Technology, Dept. of Chemistry, 1994.
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Protein engineering for cancer therapy
… of this work, we present a novel multi-agent protein-based system for targeted siRNA delivery that provides potential advantages over other nanoparticle- and proteinbased delivery vehicles. In the first agent, the double stranded RNA binding domain (dsRBD) of human protein kinase R is used as …
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Protein Engineering for Biomedical Materials
The inherent design freedom of protein engineering and recombinant protein production enables specific tailoring of protein structure, function, and properties. Two areas of research where protein engineering has allowed for many advances in biomedical materials include the design of novel protein …
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Protein Engineering via in Vitro Coevolution
The creation of novel protein functions represents a significant challenge in protein engineering. To address this challenge, I have developed a simple and efficient method---in vitro coevolution---for the creation of novel protein functions in an existing protein scaffold. The in vitro coevolution …
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Protein Engineering for Biomedicine and Beyond
… specificity and selectivity for their target. Protein engineering enables the design of scaffolds that can bind targets of interest while increasing their stability, and expanding the scope of applications in which these scaffolds will be useful. Repeat proteins are instrumental in a wide …
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Machine learning meets and enhances protein engineering
… pivotal role in modern science, and the study of proteins is equally vital due to their essential biological functions. In my research, I propose new machine learning models and algorithms, including novel generative models, advanced data augmentation techniques, innovative model architecture …
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Learning hierarchical motif embeddings for protein engineering
… the evolutionary analysis, search and design of proteins, based on a hierarchical decomposition of proteins into a set of functional motif embeddings. We introduce, CoMET - Convolutional Motif Embeddings Tool, a machine learning framework that allows the automated extraction of nonlinear motif …
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Metabolite biosynthetic enzymes: catalysis, substrate specificity and protein engineering
… In the biotin biosynthetic pathway, the protein in charge of these two reactions is BioW, an adenylating enzyme that represents a new protein fold within the superfamily of adenylating enzymes. Here I show substrate-bound structures that identified the enzyme active site and elucidated …
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Protein engineering for targeted delivery of radionuclides to tumors
… to the patient. We have used rational engineering design and mathematical modeling to develop a novel pretargeted radioimmunotherapy (PRIT) approach for the treatment of cancer. In PRIT, a bifunctional antibody is administered and allowed to bind to a cancer antigen. After sufficient …
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Protein engineering design principles for the development of biosensors
Investigating protein location and concentration is critical to understanding function. Reagentless biosensors, in which a reporting fluorophore is conjugated to a binding scaffold, can detect analytes of interest with high temporal and spatial resolution. However, because these biosensors require …
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Split Inteins: From Mechanistic Studies to Novel Protein Engineering Technologies
<p>Inteins are auto-processing protein domains that carry out a post-translational process known as protein splicing. This process is characterized by excision of the intein (intervening protein) domain from within a larger polypeptide sequence with concomitant ligation of the flanking extein ( …
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Protein engineering of Heparinase I : elucidation of structure-activity relationships
… Institute of Technology, Dept. of Chemical Engineering, 1996.
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In Vitro Synthetic Biology Platform and Protein Engineering for Biorefinery
… a feasible biomanufacturing platform has great engineering, and can be used to convert renewable biomass resources to a spectrum of marketable products and renewable energy. As future efforts are addressed to overcome remaining challenges, for example, decreasing enzyme production costs, …
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Protein engineering of cota laccase by using bacillus subtilis spore display
… cell-surface display systems. For instance, protein-folding problems associated with the expressed recombinant polypeptide crossing membranes are avoided. Hence, a different region of protein space can be explored that previously was not accessible. In addition, spores tolerate many …
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Exploring and Exploiting Ribonuclease 1: from Protein Biochemistry to Protein Engineering
Ribonuclease (RNase) 1 is a human protein with a remarkable ability to indiscriminately hydrolyze RNA. RNase 1 and its bovine homologue RNase A exhibit ubiquitous expression across tissues, a catalytic efficiency within the diffusion-limited regime, and minimal substrate sequence requirements. …
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An application of artificial intelligence and machine vision to protein engineering
Thesis: Ph. D., Massachusetts Institute of Technology, Department of Chemistry, 1992
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Protein engineering and bioprospecting for selective hydroxyacid production in engineered Escherichia coli
Engineering of microbes can allow for the sustainable production of a variety of useful chemical compounds upon which we rely in our everyday lives. The many advantages of metabolic engineering include the use of renewable resources, mild reaction conditions, such as ambient pressure, pH and …
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Artificial intelligence and protein engineering : information theoretical approaches to modeling enzymatic catalysis
… Despite this, the vast majority of enzyme engineering approaches are based on the energetic optimization of static molecular structures. Using computational sampling techniques, especially rare-event sampling methods like Transition Path Sampling, we can now generate large datasets of …
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Pathway and protein engineering for improved glucaric acid production in Escherichia coli
Microbial fermentation is an attractive method for the renewable production of chemicals. Glucaric acid was identified as a "top value added chemical from biomass" by the Department of Energy in 2004, and a biological route for its production from glucose in E. coli was developed in our lab in …
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New protein engineering approaches for potentiating and studying antibody-based EGFR antagonism
… of the EGF receptor (EGFR), rendering this protein an attractive therapeutic target. Anti-EGFR therapeutics are a mainstay of clinical practice for the treatment of colorectal, lung and head and neck cancers but efficacy is limited and response rates low. Opportunities for improving EGFR …
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