Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 20 of 30 for “"Protein Distribution"”.
-
NUCLEAR ENVELOPE TRANSMEMBRANE PROTEIN DISTRIBUTION AND TRANSPORT STUDIED BY SINGLE-MOLECULE MICROSCOPY
… cytoplasm by forming a barrier. NE transmembrane proteins (NETs) embedded in either the outer nuclear membrane (ONM) or the inner nuclear membrane (INM) play crucial roles in both nuclear structure and functions, including: genome architecture, epigenetics, transcription, splicing, DNA …
-
Exploring Polarized Membrane Protein Distribution in Drosophila Follicle Cells using Proximity Labelling
… depend on the faithful sorting and delivery of proteins to distinct plasma membrane domains. Using the Drosophila follicular epithelium as my model, this thesis applies peroxidase-mediated proximity labelling and mass spectrometry to systematically characterize the polarized membrane proteome. I …
-
Dynamic visualization and genetic determinants of Sonic hedgehog protein distribution during zebrafish embryonic development
… cells. This is in part achieved by the proper distribution of signaling molecules, many of which exert their function by establishing gradients of concentration. Because of this property they were named "morphogens", or "form giving" substances. Among these, proteins …
-
Influence Of Cholesterol And Bilayer Asymmetry On Membrane Protein Distribution In Polymer-Tethered Raft-Mimicking Lipid Membranes
… by causing sequestering of membrane proteins. Underlying mechanisms of raft-associated membrane protein sequestration remain elusive, due to the complexity, transient nature, and small size of raft domains in cellular membranes. To address these challenges, this dissertation unveils …
-
Desert Hedgehog is a Mammal-Specific Gene Expressed During Testicular and Ovarian Development in a Marsupial
… in the avian lineage. Like other hedgehog proteins, DHH signals through the patched (PTCH) receptors 1 and 2. Here we examine the expression and protein distribution of DHH, PTCH1 and PTCH2 in the developing testes of a marsupial mammal (the tammar wallaby) to determine whether DHH …
-
Micro/Nanoencapsulation of Proteins Within Alginate/Chitosan Matrix by Spray Drying
Currently, therapeutic proteins and peptides are delivered subcutaneously, as they are readily denatured in the acidic, protease rich environment of the stomach or gastrointestinal track and low bioavailability results from poor intestinal absorption through the paracellular route. Encapsulation of …
-
Protein Contributions of the Male Accessory Organs to the Composition of Human Seminal Plasma as Determined by High Resolution Two-Dimensional Electrophoresis
… using this technique to establish "normal" protein distribution patterns in the two-dimensional polyacrylamide gel slab. In addition, time studies were performed on selected specimens to determine the effects of liquefaction on seminal plasma protein composition within the first several …
-
Characterization of a glycerophosphodiester phosphodiesterase in the human malaria parasite Plasmodium falciparum
… We generated yellow fluorescent fusion proteins that revealed a complex distribution of PfGDPD within the parasite cytosol, parasitophorous vacuole and food vacuole. To gain insight into the role of PfGDPD, sub-cellular localization was modulated and resulted in a shift in protein …
-
Biochemical Analysis of the Protein-Protein Interactions Involved in Karyopherin-Mediated Transport Across the Nuclear Pore Complex
… complex consisting of approximately 30 different proteins. Small molecules can freely exchange through the NPC, but macromolecules larger than ~40 kDa such as proteins, mRNAs, and ribosomal subunits must be aided across by shuttle proteins (karyopherins, or Kaps). Kap-mediated transport involves …
-
NIR hyperspectral imaging for animal feed ingredient applications
… a Corning NIR HSI instrument was used to predict protein and oil content in soybean meal and visualize predicted protein distribution over the entire soybean meal sample. Preprocessing by standard normal variate and Savitzky-Golay derivative was effective in improving calibration model …
-
Developmental Alterations in the Distribution of Specific Lateral Wall Proteins Modify Outer Hair Cell Mechanical Properties
… of a plasma membrane, in which the motor protein prestin is densely packed, an actin-spectrin cortical lattice and subsurface cisternae. Developmental alterations in protein distribution and localization of each of these structures were correlated with alterations in OHC compliance to …
-
Functional characterization of the role of Imp, a Drosophila mRNA binding protein, during oogenesis
… A family of highly conserved RNA binding proteins in vertebrates, VICKZ (V̲g1RBP/V̲era, I̲MP-1, 2, 3, C̲RD-BP, K̲OC, Z̲BP-1) proteins, has been shown to act in these two processes. Previous studies of the posttranscriptional mechanisms mediated by VICKZ family members have been largely …
-
Development of Novel Labelling Strategies for Imaging in Nanoscale Topography
… role in signalling outcomes, it follows that the distribution of proteins must be considered in relation to the various structures exhibited on the membrane surface. The combination of these aforementioned challenges makes 3D-SMLM uniquely placed to address some common questions within the field. …
-
Ameliorating the toxic side-effects of cisplatin by systematically modulating its metabolism in human plasma with chemoprotective agents
… plasma while concomitantly reducing the plasma protein binding of CP-derived Pt species. Analogous experiments that were carried out with PBS-buffer instead of plasma allowed to gain more insight into the bioinorganic chemistry that unfolds in the absence of plasma proteins under physiologically …
-
Depletion of docosahexaenoic acid (DHA) and arachidonic acid causes male infertility by disrupting both secretory granule biogenesis and adherens junction assembly in testis
… DHA was found to be sufficient in restoring the distribution of vesicular trafficking proteins that promote the biogenesis of an organelle unique to sperm, the acrosome. In contrast, ARA was much less effective than DHA in restoring germ cell adhesion integrity. Assessments of cell-cell contacts …
-
Investigation of protein biomarkers in HIV positive and HIV negative associated DLBCL
… Aim: We aimed to investigate in detail, protein distribution profile from FFPE tissue in HIV and non-HIV related DLBCL subtypes. Methods: FFPE DLBCL lymph node tissue samples from HIV and non-HIV related DLBCL were subjected to MALDI-imaging, in order to get the spatial distribution of …
-
Investigating spatial distribution and dynamics of membrane proteins in polymer-tethered lipid bilayer systems using single molecule-sensitive imaging techniques
… assemblies comprised of lipids and membrane proteins. Both types of membrane constituents are organized in highly dynamic patches with profound impact on membrane functionality, illustrating the functional importance of plasma membrane fluidity. Exemplary, dynamic processes of membrane protein …
-
Rho GTPase family members in establishment of polarity in C. elegans embryos
… conserved partitioning-defective (PAR) proteins as well as the actin cytoskeleton. In Caenorhabditis elegans one-cell embryos, the PAR proteins are segregated into an anterior (PAR-3, PAR-6) and a posterior (PAR-1, PAR-2) corticaldomain. The formation of PAR polarity correlates with …
-
An analysis of the nutrition support process in rugby union athletes in New Zealand: Practical considerations and applications within rugby developmental environments
… with caution. In Chapter 5, the daily distribution patterns of professional and semi-professional rugby players were investigated. Rugby players may benefit from consideration of protein distribution, with mechanistic research suggesting an even spread of 0.4g‧kg‧d across 4 – 6 meals …
-
A histopathological and genomics study of the mutated human FAM111B gene related POIKTMP disease
… the human FAM111B gene. FAM111B gene codes for a protein whose function is not well characterized. Therefore, elucidating the mechanism of FAM111B or its mutations in POIKTMP is beneficial to understanding the complexities surrounding this multisystemic fibrosing disease. The study sought to …
Page 1 of 2