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Showing 1 to 12 of 12 for “"Protein Diffusion"”.
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Measurement of rapid protein diffusion in the cytoplasm by photoconverted intensity profile expansion
Whether at the level of a single protein, or in the cytoplasm as a whole, the diffusive mobility of proteins plays a key role in biological function. To measure protein diffusion in cells, researchers have developed multiple fluorescence microscopy methods, and have tested them rigorously. However, …
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Diffusion of proteins inside crowded structures generated using microemulsions
… the degradation agent and can be eliminated. The protein diisopropyl-fluorophosphatase (DFPase) is a promising candidate for decontamination applications and is able to degrade different warfare agents. Therefore, the knowledge of the dynamics and properties of enzymes inside a bicontinuous …
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Protein folding and diffusion: from in vitro to live cells
Protein folding landscapes and protein-protein interaction landscapes are subject to modulation by many factors inside living cells: crowding, electrostatics, hydrophobic interactions, and even hydrodynamic phenomena. The resulting spatio-temporal fluctuations in protein folding rates, protein …
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Poly(ethylene glycol) hydrogel microspheres as a controlled release device
… study was performed to determine whether the diffusion of the chemoattractant could be controlled by varying the amount of crosslinker and by incorporating ionic groups in the polymer matrix. It was found that the crosslinker amounts successfully altered the release profiles of the protein. …
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A neuron within a neuron: the effect of narrow axonal ER tubules on molecular movement
… axons. A common cause of HSP is the mutation of proteins that shape the tubular endoplasmic reticulum (ER). The architecture of the ER tubular network that extends through axons is therefore likely to be integral to the maintenance and survival of axons. The continuity and ubiquity of the ER …
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Advances in biophysical methods for protein detection and characterisation
Proteins are the building blocs of life and mediate nearly every function in the cell. They are therefore a major and incredibly wide research topic. Their functions and malfunctions have serious impacts on a wide range of diseases. Proteins may be used as extremely versatile tools for biology such …
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Fluorescence-force spectroscopy at the single molecule level
… DNA four-way (Holliday) junction; 2) to dissect protein diffusion and dissociation mechanisms on single stranded DNA, 3) to calibrate FRET-based in-vivo force sensors and 4) to study mechanical unfolding of single proteins. The results could not have been obtained with fluorescence or force …
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Single-molecule and ensemble studies of DNA replication system
… to reconstitute the DNA replication machinery protein by protein. In the first study, by developing a real-time fluorescence assay, we discovered that RFC can assemble a PCNA ring from monomers in solution. A motion-based DNA polymerization assay showed that the PCNA assembled by RFC is …
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Mathematical modelling of negative feedback signal transduction processes
… molecules, which is mainly governed by proteins reacting with each other.<br/>Intracellular signalling networks are mainly based on kinases and phosphatases, enzymes which control phosphorylation and dephosphorylation of other enzymes in the cellular surrounding to the nucleus.<br/>In …
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Rotational motion and organization studies of cell membrane proteins
… Plasma membrane receptors are integral membrane proteins with diverse structures and functions which bind specific ligands to trigger cellular responses. Due to compartmentalization of the plasma membrane and the formation of membrane microdomains, receptors are distributed non-homogeneously in …
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Dynamics and equilibria of weak protein interactions in cells
Submission published under a 24 month embargo labeled 'U of I Access', the embargo will last until 2025-08-01