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Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 33 for “"Protein Denaturation"”.
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Lysozyme encapsulated gold nanocluster for studying protein denaturation
Protein denaturation is a change in a protein's structure from its native folded state to a non-native misfolded state. Protein denaturation is the cause of many diseases. Current methods used for protein denaturation studies have provided useful information regarding protein structures, but have …
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The rigor bond’s interaction with protein denaturation : effects on water-holding of myofibrils
… understand the rigor bonds’ effect together with protein denaturation on water-holding capacity by breaking the actomyosin bonds that were formed during rigor process and examining the dissociation of actomyosin bonds’ influence on rheological features of myofibrils in suspension. In this study …
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High Pressure Protein Denaturation: Description of a High Pressure Intermediate and Effects of Mutations on Pressure Stability
… specific packing defects affect high pressure denaturation. Mutations were made in three positions in the core to determine how the position contributes to changes in stability. Also, charged and oversized residues were introduced into the core to determine how particular types of defects …
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Effects of drying and heating conditions on protein denaturation, starch damage and protease activity of corn: Its relationship to wet millability
… on starch damage (gelatinization) and protein denaturation of individual corn kernels. For different genotypes, starch damage was found to increase as kernel temperatures, harvest moisture and heating time increased; hard genotypes showed more distorted granules than the soft genotype. …
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The Effects of Irradiation and Antioxidants on Myoglobin Derived From Beef Hearts
… range (400--700 nm) and absorbance at 280 nm. Protein denaturation, protein concentration, and met- (MMb), deoxy- (DMb) and oxy- (OMb) myoglobin percentages were calculated from reflectance and absorbance data. Antioxidants influenced color, % protein denaturation, and % protein concentration. …
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The influence of rapid pH decline and high muscle temperature on the water holding capacity and softness of beef
… temperature post-mortem would lead to increased protein denaturation, reduced WHC , accelerated meat tenderisation and shortening of sarcomeres and hence soft and exudative meat. It was also hypothesised that these effects would be dependent on the muscle fibre type. In one of the experiments, …
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Chemical and physical changes resulting from UHT treatment of milk
… of the caseins and the heat denatured serum proteins. A fluorometric method of analysis for total sulfhydryl content of milk serum was developed that was rapid and sensitive to changes in parts per billion. Quantitative changes in total sulfhydryl at the different ultra-high temperature (UHT) …
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Development of a novel cyanide-free method for analysis of vitamin B₁₂ in milk-based infant formula
… to eliminate or limit interferences, including protein denaturation and sugar removal using C₁₈ solid phase extraction. The final analyte was quantified using hydrophilic interaction liquid chromatography with fluorescence detection. The single laboratory validation experiment showed that this …
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Tumble Marination Strategies to Improve pH, Color, and Water-Holding Capacity in Pale, Soft, and Exudative (PSE) Broiler Breast Fillets
… are still high. This decrease in pH leads to protein denaturation attributing to the pale color and poor water holding capacity (WHC) that is characteristic of this lesser quality meat. Pale, soft, and exudative meat has economic implications for processors with losses ranging between $2-4 …
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A fundamental investigation of surface-induced skin irritation
… the outer layer of the skin, and denaturing proteins in the epidermis and the dermis. The goal of this thesis has been to understand the relationship between the physicochemical properties of surfactant solutions and their skin irritation potential. In vitro tests were developed to measure: …
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Biomedical ultrasonics, cavitation, and sonoporation
… can heat tissue to a temperature that causes protein denaturation and coagulative necrosis. For high-resolution focused ultrasound microsurgery, high working frequencies are necessary. We manufactured a highfrequency, high-intensity focussed ultrasound transducer, using lithium niobate as the …
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Structure and activity of protein-nanoparticle conjugates: towards a strategy for optimizing the interface
Nanoparticle-protein conjugates have a variety of applications in imaging, sensing, assembly and control. The nanoparticle-protein interface is made of numerous complex interactions between protein side-chains and the nanoparticle surface, which are likely to affect protein structure and compromise …
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Role of dry beans (Phaseolus vulgaris L.) in binding bile salts and modulating lipid digestion: Impact of the bean matrix and high-hydrostatic pressure processing
… HT caused complete starch gelatinization and protein denaturation of beans, while HHP treatments induced partial or no starch gelatinization and a lower degree of protein denaturation, which resulted in enhanced protein solubility and emulsifying activity/stability. It was observed that, while …
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Structural Comparison of Denatured and Supercharged Proteins in Gas and Solution Phases
… methods allow for top-down analysis to determine protein structures. As a gas phase technique, it is crucial to understand how ions fold or unfold in the gas phase without the support of solvents/water, as well as how they behave during the various energy and pressure changes experienced …
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Controlling the denaturation and aggregation of whey proteins using κ-casein and caseinomacropeptide
Whey proteins ingredients are extensively used in a variety of product formulations such as dairy beverages, infant formula and sport nutritional beverages, due to their nutritional and functional properties. Dairy protein-containing beverages are thermally processed, typically to ensure …
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Physicochemical Properties and Antioxidant Activity of Enzymatic Modified Soy Protein Isolate Films with Lignin
… to provide food safety and security. Soy protein isolate (SPI) was enzymatically modified by transglutaminase under different conditions to ensure desirable and optimized enzyme crosslinking activity before film preparation. Physicochemical properties including viscosity and molecular …
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Reductive metabolism of aliphatic tertiary amine n-oxides.
… sensitive and can be suppressed by CO, CN- and protein denaturation. Under anaerobic conditions, in vitro lignocaine N-oxide reduction was found to occur in NADPH supplemented rat liver homogenates, microsomal suspensions; rat heart homogenates, cytosolic solutions; human phenotyped cytochrome …
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Ante- and post-mortem factors affect muscle protein functionality from fish
… Brining time linearly increased myofibrillar protein solubility, fillet weight loss after brining, water-phase salt content, and cooked ash content and linearly decreased water activity and brined and cooked moisture content. An 8.7% (w/v) brine and 90-min brining time resulted in maximum …
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Exopolysaccharides Modify Functional Properties of Whey Protein Concentrate
… objectives of this research were to produce whey protein concentrate (WPC) with modified functionality using exopolysaccharides (BPS) producing cultures and utilize modified WPC to replace egg whites in cake. Two different BPS-producing cultures, Lactococcus lactis ssp. cremoris (JFR) and …
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