Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 23 for “"Protein Crystallography"”.
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Extending the utility of enzymes for site-specific targeting of fluorescent probes
… reporters such as the green fluorescent protein (GFP) are useful for studying protein expression, localization, and dynamics in a variety of biological systems. GFP and its related variants, however, suffer from several drawbacks. Compared to chemical fluorophores, they are large, dim, …
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Oxidative stress responses in Campylobacter jejuni: the role of the Peroxide Regulator PerR
… campylobacteriosis. One of the main regulatory proteins involved in the protective response of C. jejuni to oxidative stress is the regulatory protein PerR, which regulates gene expression in a metal-dependent manner, controlling the transcription of a set of peroxidases. In this study the perR …
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Staphylococcus aureus DNA gyrase: mechanism and drug targeting
… sites located in N-terminal domain of GyrB by protein crystallography has suggested a novel role of these M+ ions in the supercoiling functions of DNA gyrase, providing theoretical links to the unique KGlu dependency in S. aureus gyrase and the dependency of monovalent ions in E. coli and …
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Design and Synthesis of Inhibitors of Mycobacterium tuberculosis Menaquinone D (Mtb MenD) as Potential Therapeutics for Tuberculosis (Tb)
… silico inhibitor docking and molecular design, protein crystallography, enzyme kinetics and microbiology. Initially, a series of inhibitor candidates derived from the natural substrate isochorismate were synthesized. Unexpectedly, these were shown by X-ray crystallography to bind to a previously …
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Development of a high throughput screen for the expression of membrane proteins and their purification and crystallisation
… environment and as a support matrix for membrane proteins. Membrane proteins facilitate the transport of manifold substrates across the membrane and are involved in fundamental cellular processes, such as signalling or energy generation to name a few. The key to the function of membrane proteins …
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Kristallstruktur einer Phenylalanin-Ammoniak-Lyase und Kristallisation einer Tyrosin-Aminomutase sowie Komplexstrukturen der Ecto-ADP-Ribosyltransferase ART2.2
… PAL ist ein fast ausschließlich alpha-helicales Protein mit 23 teilweise sehr langen alpha-Helices und acht kurzen beta-Faltblattsträngen. Ein PAL-Protomer besteht aus 716 Aminosäureresten und kann in eine MIO-Domäne, eine core-Domäne und eine shielding-Domäne unterteilt werden. In der …
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Biochemical and synthetic chemical approaches to studying the biosynthesis and regulation of bacterial secondary metabolites
… efforts to understand the catalytic mechanism of proteins involved in the biosynthesis of the medically active natural product roseoflavin (RoF) and studying the mechanism to understand the regulatory action of a small signaling molecule, avenolide, in avermectin production. The research projects …
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Investigation into the structure and function of Hsp47
Collagen is the most abundant protein in mammals and is the main protein of connective tissue in animals [1]. It is a fibrous structural protein that is the major component of the extracellular matrix, which provides great tensile strength to ligaments, tendons, bone and skin, for example. Hsp47 is …
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Exploring the impact of detection physics in X-ray CCD imagers and spectrometers
… synchrotron based research programmes, such as protein crystallography and X-ray diffraction imaging, X-rays are used, once again, for imaging purposes. In both cases, a high-resolution detector with a high frame-rate is required such that images can be taken with a spatial resolution of the …
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Molecular basis of the evolution of new protein functions: Kemp elimination and phosphoryl transfer reactions
… into the molecular basis of the evolution of new protein functions. The broad objective of this work was to determine exactly how a series of single amino acid mutations, typical of an evolutionary trajectory, can result in dramatic changes in catalytic activity, specificity and protein …
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Molecular basis of the evolution of new protein functions: Kemp elimination and phosphoryl transfer reactions
… into the molecular basis of the evolution of new protein functions. The broad objective of this work was to determine exactly how a series of single amino acid mutations, typical of an evolutionary trajectory, can result in dramatic changes in catalytic activity, specificity and protein …
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The Mechanism of N10-Formyltetrahydrofolate Synthetase. Use of Human Thymidylate Synthase Variants to Characterize Asymmetric Ligand Binding and to Identify Novel Allosteric Inhibitors.
… the interaction of hTS with nuclear transport proteins allows for the design of other allosteric inhibitors, which is a novel approach to hTS inhibition.</p>
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Metabolite biosynthetic enzymes: catalysis, substrate specificity and protein engineering
… In the biotin biosynthetic pathway, the protein in charge of these two reactions is BioW, an adenylating enzyme that represents a new protein fold within the superfamily of adenylating enzymes. Here I show substrate-bound structures that identified the enzyme active site and elucidated …
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Structure Determination of Natural Product Biosynthesis Enzymes
… interrogation of the synthetic and regulatory protein machinery. Structural and functional pursuit of NP associated proteins sheds light on the molecular mechanisms governing intricate regiospecific and stereospecific modifications laying the foundation for future bioengineering and synthetic …
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Microfluidic platforms for membrane protein crystallization and in situ crystallography
Membrane proteins, biological macromolecules that reside in cellular membranes, play critical roles in many biological process, including signaling, transport, and intercellular communication. The malfunction of membrane proteins has been linked to the initiation or progression of many diseases …
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Multimethodological approaches to tackle glycogen: therapeutic and modelling opportunities in Lafora disease
Protein aggregation is a key pathological hallmark of many neurodegenerative diseases. However, such a paradigm does not universally prevail. Indeed, some neurological glycogen storage disorders manifest through the accumulation of aberrant glycogen into polyglucosan bodies (PGBs). These include …
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Development and Optimisation of an Optofluidic Evanescent Field Nano Tweezer System for Trapping Nanometre Crystals for Synchrotron X-Ray Diffraction Experiments
X-ray crystallography (the analysis of the diffraction pattern of a high-power X-ray) has become the preferred method for investigating complex molecular structures. Research has continued to improve both the hardware and software elements of this methodology; refining critical factors such as …
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Röntgenstrukturanalyse der 6-Hydroxy-D-Nikotin-Oxidase aus Arthrobacter nicotinovorans sowie Darstellung und Charakterisierung einer Protein-Protein-Kontaktfläche mit der 6-Phospho-ß-Galaktosidase aus Lactococcus lactis
Die 6-Hydroxy-D-Nikotin-Oxidase (6HDNO) aus Arthrobacter nicotinovorans wurde rekombinant exprimiert, gereinigt und kristallisiert. Die Struktur wurde mit röntgenkristallographischen Methoden in drei Kristallformen bis zu einer Auflösung von 1.9 Å gelöst und verfeinert. Das Enzym katalysiert die …
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Structural Studies of Two Enzymes of Pantothenate Biosynthesis in Escherichia Coli
… to determine the mode of cofactor binding to the protein, ketopantoate hydroxymethyltransferase was crystallized in the presence of two 5,10-methylene-5,6,7,8-tetrahydrofolate analogues and alpha-ketoisovalerate. X-ray diffraction patterns, collected on the in-house X-ray diffraction data …
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The Automatic Detection of Small Molecule Binding Hotspots on Proteins: Applying Hotspots to Structure-Based Drug Design
Locating a ligand-binding site is an important first step in structure-guided drug discovery, but current methods typically assess the pocket as a whole, doing little to suggest which regions and interactions are the most important for binding. This thesis introduces Fragment Hotspot Maps, a …
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