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Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 12 of 12 for “"Protein Conjugation"”.
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Protein Synthesis and Bioconjugation for Design of Antimicrobial Conjugates
… The first strategy, chemical synthesis of proteins, enables access to proteins equipped with modifications and conjugation handles that are valuable for unlocking their therapeutic potential. We used this approach to produce a synthetic antipseudomonal bacteriocin and demonstrated that its …
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Part I. Cleavage of Carbamates to Isocyanates: Selectivity, Extension to the Solid Support, and Synthesis of Multivalent Glycoforms With Inter-Saccharide Carbamate Linkages. Part II. Conjugation of Carbohydrate Moieties to a Rigid Aromatic Linker for the Study of Glycoside Internal Dynamics
… of carbohydrate-linker moieties that will allow conjugation of a saccharide to a protein is reported. This approach was demonstrated in the synthesis of the saccharide-linker moieties of the four glucopyranosyl-beta-D-glucopyranosides. The results show that disaccharides with varying glycosidic …
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Fibrin targeting microbubbles for the detection of nascent surgical adhesions
… the effect of lipid saturation and targeting protein conjugation on microbubble stability and surface charge. The binding specificity of the targeted microbubbles was shown by comparing the binding of targeted and non-targeted bubbles to a fibrin gel. Finally, histological staining techniques …
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Design of multi-functional hydrogel for cell therapies
… (3) The hydrogel was modified with cell adhesion proteins independent of other hydrogel properties using an amine-reactive polyaspartamide linker enabling single-step protein conjugation. Furthermore, mineralization capacity of the hydrogel was controlled independently by modulating …
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Patterned substrates for cell culture with electrohydrodynamic jet printing
… is introduced as a method to pattern adhesion proteins on hydrogel substrates for cell culture. First, a new technique to fabricate polyacrylamide substrates was developed and optimized. Hydrogels were formulated with acrylic acid and activated with N-hydroxysuccinimide. Protein density was …
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Visible Light-Mediated Polymerization for Protein-Polymer Conjugation: A Biocompatible Tool for Therapeutic Applications
Proteins are natural biopolymers composed of amino acids, holding significant roles in both industry and medicine towing to their high efficiency and selectivity. Conjugating synthetic polymers to proteins results in novel hybrid macromolecular structures, which synergistically combine the benefits …
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Studies and applications of protein prenylation using isoprenoid analogues and site-directed mutagenesis
The site-specific enzymatic modification of proteins offers a powerful tool both for the development of new research techniques and for the development of more effective therapeutics. Protein farnesyltransferase (PFTase) is a promising tool in the field of site-specific protein modification due to …
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Development of novel polymeric nanoparticles for pancreatic cancer therapy
… is explored in the following thesis through conjugation of shark-derived immunoglobulin fragments known as Variable New Antigenic Receptors (VNARs) to the surface of polymeric NPs. This is a novel use of VNARs for which they have many advantageous properties including high physicochemical …
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Proteinkonjugationen durch Kupfer-abhängige Formylglycin-generierende Enzyme zur Generierung von Antikörper-Wirkstoff-Konjugaten
… enzyme (FGE) is utilized for the regioselective conjugation of proteins. In an oxygen-dependent reaction it catalyzes the formation of the non-canonical amino acid Cα-formylglycine (FGly) from the cysteine residue of a CxPxR-type core recognition se-quence. In addition to cysteines, the …
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Nanoparticle Biofunctionalization for Self-Assembly and Energy Transfer Applications
… dots (QDs), and quantum rods (QRs) with DNA and proteins for self-assembly, energy transfer and drug delivery applications.</p> <p>In the first project, we explored the ability to assemble QDs into clusters with a high degree of control through DNA-mediated interactions. The hydrophobic QDs were …
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Preparation, characterisation and functional applications of whey protein-carbohydrate conjugates as food ingredients
… technological hurdles limiting the use of whey protein ingredients in value-added nutritional beverages include poor solubility, heat stability and lack of clarity, particularly in high-acid or high ionic strength, ready-to-drink systems such as those found in sports and performance nutritional …