Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 13 of 13 for “"Protein Conformations"”.
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Riemannian geometry for inverse problems in cryogenic electron microscopy
… orientation estimation and that of modelling of protein dynamics. The thesis is concluded with a discussion on how to bring these pieces together to solve the so-called continuous heterogeneous reconstruction problem and a reflection on the general implications and new opportunities that a …
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Exploring Novel Immunodiagnostics for Prion Disease
… diseases occur when the normal cellular prion protein (PrPC) misfolds, producing the infectious isoform PrPSc,which can readily self propagate with no nucleic acid intermediate. PrPSc aggregates are insoluble self-molecules, which results in a large number of limitations pertaining to the …
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Advances in Computational Solvation Thermodynamics
… measurements used to analyze the stability of protein conformations in aqueous milieus. Solvation free energies govern the favorability of the solvation process, while entropy and enthalpy decompositions give insight into the molecular mechanisms by which the process occurs. Computationally, a …
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Effect of Dried Mushroom Powder on Thiol Content in Lyophilized and Native Bovine Myofibrillar Protein
… oxidation can form disulfide bonds that modify protein conformations, resulting in lower free thiol content. The effect of dried (lyophilized and oven-dried) mushroom powder<em> </em>(<em>Agaricus bisporus </em>or <em>Pleurotus ostreatus)</em> added to bovine lyophilized and native myofibrillar …
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Computational methods to design biophysical experiments for the study of protein dynamics
… and data acquisition. In the field of protein biophysics, computational simulations such as molecular dynamics and spectroscopy-based experiments such as double electron-electron resonance and Fluorescence resonance energy transfer techniques have emerged as critical experimental tools …
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Exploring the Structural Dynamics of Bacterial Chemotaxis
… scale ruler that enables the observation of protein conformations and dynamics, was applied to intact, functional chemoreceptor homodimers to observe ligand-induced changes. This thesis describes work to interrogate the structural dynamics of chemoreceptor homodimers associated with ligand …
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Computational dissection of nitrate transport mechanism
… biology only provides few ""snapshots"" of protein structures, whereas proteins undergo complex dynamical processes to perform their functions. Molecular dynamics (MD) simulations are a powerful tool to decipher the mechanism of nitrate transporters because they can capture the dynamical …
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Roles of cosolvents on protein stability
The function of a protein is determined by its three-dimensional structure which emerges from the delicate balance of forces involving atoms of the protein and the solvent. This balance can be perturbed by changing temperature, pressure, pH and by adding organic molecules known as cosolvents to the …
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Studies into solid core drug delivery system using haemoglobin as a model drug and supercritical fluid processing for encapsulation
… core drug delivery system for oral delivery of proteins using silica as core material, haemoglobin as model drug and supercritical fluid processing as encapsulation technique. Silica particles of different morphology were used as a carrier material for protein immobilisation and fatty acids …
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Protein characterization using a computational microscope: Nanopore sequencing and biological condensates
Submission published under a 24 month embargo labeled 'Closed Access', the embargo will last until 2025-08-01
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Using Protein-Likeness to Validate Conformational Alternatives
<p>Proteins are among the most complex entities known to science. Composed of just 20 fundamental building blocks arranged in simple linear strings, they nonetheless fold into a dizzying array of architectures that carry out the machinations of life at the molecular level.</p><p>Despite this …
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Energetics and mechanisms of substrate transport by the MATE transporter PfMATE
… with both outward-facing and inward-facing protein conformations determined. Although advanced techniques have brought insights into the structural features of PfMATE, the intricate details of its transport mechanism, including substrate selection, ion coupling, ion-drug stoichiometry, and …
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Development and Applications of Liquid Sample Desorption Electrospray Ionization Mass Spectrometry (DESI-MS)
… sample DESI are discussed in details including protein conformation study, fast reaction kinetics study, and the coupling with LC for enhancing protein signal and charge. In liquid sample DESI, direct ionization of continuous-flow liquid samples was achieved without the sample drying. In this …