Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 20 of 30 for “"Protein Assemblies"”.
-
Biophysical Studies of Protein Assemblies
Proteins are synthesised as linear polymeric chains. The subtle energetic interplay of interatomic interactions results in chain folding, through which proteins may acquire defined structures. This spatial organisation is encoded by the protein sequence itself; the so-called thermodynamic …
-
Hierarchical protein assemblies with biomedical applications
… materials are generated. Among these processes, protein self-assembly into hierarchical structures provides an important avenue for the development of materials with a wide range of biomedical applications. Self-assembly exploits the formation of strong, non-covalent interactions between …
-
Synthetic scaffolds and protein assemblies for engineering applications
S-layer proteins, which naturally self-assemble on the exterior of cells, provide an interesting basis for the creation of synthetic scaffolds. In this thesis, I created a plasmid which produces a recombinant form of a well characterized S layer protein, sbpA, which has a number of properties ideal …
-
Molecular Investigations of Protein Assemblies Involved in Prokaryotic Virulence
Protein complexes mediate a diverse range of behavior in prokaryotic cells, yet the exact molecular mechanisms explaining how many of these complexes assemble and function remain unknown. This work focuses on understanding the molecular mechanisms of two different protein assemblies responsible for …
-
Protein MAS NMR methodology and structural analysis of protein assemblies
… with particular emphasis on the analysis of protein structure, are described in this thesis. MAS NMR studies of biomolecules ranging from model peptides and proteins in crystalline form to amyloid fibrils and whole bacterial organelles are reported. The methods presented include novel pulse …
-
A New Generation of Idealized Protein Building Blocks Enables Rational Nanomaterial Design
… work with them. In contrast, the design of novel protein nanomaterials has been difficult due to the irregular shapes and behaviors of naturally-evolved proteins. Even when new protein complexes can be modeled, it remains a challenge to assign sequences to the models for them to form correctly …
-
Tunable Microchips for Imaging Protein Structures formed in Breast Cancer Cells
The breast cancer susceptibility protein, BRCA1, is a tumor suppressor that helps maintain genomic integrity. Changes in BRCA1 that effect DNA repair processes can fuel cancer induction. The Kelly lab, at the Virginia Tech Carilion Research Institute, has recently developed a new methodology that …
-
Computational Modeling and Analysis of Protein Interactions at Multiple Coarse-grained Levels in Biological Systems
Proteins, composed of unique amino acid sequences, fold into compact, functional structures. These proteins often form large complexes that interact with other proteins and genes to regulate cellular processes. Studying these interactions is challenging due to their transient nature. This thesis …
-
Insights into structure, dynamics of thioredoxin reassembly and dynein light chain 8 and ionization states of thiamin cofactor by MAS NMR spectroscopy
… atomic-resolution structural characterization of proteins and protein assemblies that are insoluble and difficult to crystallize. A number of laboratories including ours are working on developments of MAS technologies that would enable analysis of complex biological systems. In this dissertation, …
-
Generative Diffusion Models Towards De Novo Protein Design
De novo protein design aims to generate proteins with desired functions by rationally engineering novel protein structures and sequences. The structure requires modeling continuous 3D coordinates of atoms with rigid biochemical constraints of the polymer chain while the sequence is a series of …
-
PNA-protein conjugates for nano-scale modeling of protein aggregates
Abstract Programmable assembly of proteins on molecular frameworks requires the development of facile and orthogonal chemical approaches and molecular scaffolds. In this research, the unique characteristics of PNA were applied to create controllable protein assemblies directed by precise PNA-DNA …
-
Phase transitions in soft matter: From colloidal suspensions to biomolecular condensate regulation
… find common ground with the dynamic behavior of protein liquid-liquid phase separation by studying biophysical principles with different colloidal computational models for biomolecules. Our study of protein assemblies will therefore resemble previously investigated liquid-vapor transitions, …
-
Computational Modeling of Claudin-15 Paracellular Channels
… in epithelial tissues through claudin protein assemblies, yet the molecular basis of strand flexibility, pore architecture, and ion selectivity for cation-selective claudin-15 (Cldn-15) remains incompletely defined at atomic resolution. This dissertation employs molecular dynamics (MD) …
-
Biochemical analysis of proteins in the telomere maintenance pathway
Eukaryotic linear chromosomes culminate in nucleoprotein structures designated telomeres. The terminal telomeric DNA consists of tandem repeats of a G-rich motif that is established and maintained by the action of the specialized reverse transcriptase called telomerase. In addition to the function …
-
Mechanism of Mcm10 function during DNA replication
… by a multi-stage process involving numerous protein assemblies. The core component of this process is the replicative DNA helicase, Mcm2-7. Mcm2-7 complexes are loaded around origins of DNA replication during G1 phase. As cells transition from G1 to S phase, the Mcm2-7 helicases are activated …
-
Microfluidic diffusional sizing at the single molecule level
… much suited to measuring the size of individual proteins and higher-order protein assemblies, and MDS has indeed been applied to good effect to study proteins and the strength of their interactions. However, conven- tional MDS is inherently an ensemble level method - it is performed in the …
-
Methods and Applications of Computational Design in Multiple States
… flexibility and stability is a key property of proteins, contributing to the vast area of structures and functions observed in nature. Flexibility arises from the complex energy landscape that determines the available conformational states of a protein. Over the past two decades, our ability to …
-
MOLECULAR MECHANISMS OF THE FORMATION OF ENDOCYTIC PATCHES AND THE INITIATION OF ACTIN POLYMERIZATION IN FAST ENDOPHILIN MEDIATED ENDOCYTOSIS
… of the β1-AR, facilitating the formation of protein assemblies mimicking endocytic priming sites. This phase transition is triggered by multivalent interactions between EDP’s SH3 domain and the proline rich motifs found in its binding partners. The availability of the receptor TIL, which is a …
-
Programmable Assembly of Elastin-like Polypeptides Within Droplet Microenvironments
<p>Disordered proteins lack a defined secondary and higher order structure, possess a large number of biological functions, and are prevalent throughout nature. One important property of certain disordered proteins in biology is an ability to phase separate within the interior of a cell and form …
Page 1 of 2