Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 256 for “"Protein A"”.
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Antibody Purification from Tobacco by Protein A Affinity Chromatography
… of producing large quantities of recombinant proteins, and tobacco is arguably the most promising plant for plant-made-pharmaceuticals (PMP) due to its high biomass yields and robust transformation technology. However, to produce proteins using transgenic tobacco for human applications, …
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Cloning and Characterization of Replication Protein A from Dictyostelium discoideum
The gene encoding the Dictyostelium replication protein A large subunit (DdRPA1) has been cloned by screening of an EcoR I partial genomic library and a Hind III genomic sub-library. The complete nucleotide sequence, including the promoter region of the gene has been obtained by sequencing. Though …
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The functions of pregnancy-associated plasma protein A in rheumatoid arthritis.
Pregnancy associated plasma protein A (PAPP-A) is produced in increasing amounts with advancing pregnancy, a major source being the placenta. It was originally described as a homotetramer with ~ 200,000 M[r] subunits. An alternative structure has since been proposed of a disulphide bridged complex …
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The molecular characterisation of pregnancy-associated plasma protein-A (PAPP-A).
PAPP-A is a large glycoprotein with alpha2-electrophoretic mobility that is produced by the placenta during pregnancy. In this thesis a biochemical and molecular characterisation of PAPP-A was performed. The polyclonal antiserum (DAKO) directed against PAPP-A has been shown to also interact with …
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The role of surfaceant protein A in immunity to HPV16 pseudovirus infection
… HPV recognition by the immune system. Surfactant proteins A and D (SP-A and SP-D) are innate immune proteins with a variety of functions including recognition and opsonisation of pathogens. They are primarily found in the lung, but have also been shown to be expressed at other sites of the body, …
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Development of methods for the detection Staphylococcal Protein A in ICU patient urine
… in the Intensive Care department. Staphylococcal Protein A (SpA) is an immunoglobulin-binding surface protein, expressed by more than 98.9% of S. aureus strains, with a primary function of aiding S. aureus in the colonisation of the host. While many Staphylococcal toxins are excreted by the …
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Preparation and characterization of Staphylococcal protein A adsorbents for high performance affinity chromatography
… HPAC. Columns were prepared by immobilizing Protein A from Staphylococcus aureus on 10 m diol-bonded silica. Immunoglobulin (Ig)-containing samples were injected into the column at pH 7 and the non-retained components eluted. The retained proteins were then eluted by a step change to pH 3. …
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Novel Roles of Replication Protein A Phosphorylation in Cellular Response to DNA Damage
<p>Human replication protein A (RPA) is an eukaryotic single-stranded DNA binding protein directly involved in a variety of DNA metabolic pathways including replication, recombination, DNA damage checkpoints and signaling, as well as all DNA repair pathways. This project presents 2 novel roles of …
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Characterisation of surfactant protein a as a novel prophylactic means against oncogenic HPV infections
… our previous research identified surfactant protein A (SP-A), an innate immune opsonin, as a novel molecule capable of recognising HPV16 pseudovirions (HPV16-PsVs) with functional consequences for reduced infection in a murine cervicovaginal HPV challenge model. Building on these findings, …
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Regulation of phoPR and cydABCD promoters of Bacillus subtilis by catabolite control protein A (CcpA).
… of Bacillus subtilis by catabolite control protein A (CcpA).
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Pseudomonas aeruginosa Type IV pilus and resistance to the antimicrobial properties of surfactant protein-A
… numerous innate immune cells and effector proteins. One important component of this immune system is the surfactant protein-A (SP-A), which facilitates microbial clearance by opsonization and membrane permeabilization. Pseudomonas aeruginosa (PA) is a major Gram-negative bacterial pathogen …
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Characterization of a Novel GPI-anchored Protein, a Component of Sphingomyelin-enriched Microdomains at the Golgi Complex
… station along the secretory pathway to sort proteins and lipids to their final destinations. It exists of a series of flattened cisternal membranes that are aligned in parallel to form polarized stacks. This structure is actively maintained amid a large flow of biosynthetic transports through …
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The Association Between Changes in Body Fat, Body Weight and Serum C-Reactive Protein: A Prospective Study
… and weight (BW) relate to changes in C-reactive protein (CRP) in women, while statistically controlling for possible confounders, such as age, initial body weight, and menopause status.</p> <p><strong><em>Methods and Results-</strong></em> A cohort of 150 free-living subjects was followed …
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Characterisation of human surfactant protein A and recombinant human vimentin in their modulation of HPV16 pseudovirus infection
… cancer progression are needed. Two human proteins have been identified in our laboratory that modulate HPV16 pseudovirus (HPV16-PsVs) infection in vitro, namely surfactant protein A (SP-A) and recombinant human vimentin (rhVim). Previous work suggested SP-A mediated immune recognition of …
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Regulation of Adaptive Immunity in the Lung by the Alveolar Epithelial Type II Cell and Surfactant Protein a
… II cell and its secretory product, surfactant protein A (SP-A), have been linked to roles in adaptive immunity in the lung. The discovery that type II cells constitutively express major histocompatibility complex class II (MHC II) suggested that type II cells may function to present antigen to …
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Synthesis of fluorescent analogues of a-tocopherol as ligands for the human a-tocopherol transfer protein (a-TTP) /
… with recombinant human a-tocopherol transfer protein (ha-TTP) utilizing fluorescent titration binding assays identified competent ligands for further use in protein studies. Compounds Id (C9-AO-a-Toc) and 2d (C9-NBD-a-Toc) both having nonyl alkyl chain extensions between the chromanol and …
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Entwicklung eines Lipoprotein-Impfstoffes aus Pflanzen : Produktion des rekombinanten 'outer surface protein A' (OspA) von Borrelia burgdorferi in Tabakchloroplasten
… Stellenwert bei der Produktion therapeutischer Proteine, besonders von Impfstoffen, ein. Einige dieser Proteine benötigen für ihre Funktionalität verschiedenste post-translationale Modifikation und es ist nicht bekannt, ob Pflanzen zu diesen Stoffwechselleistungen befähigt sind. In der …
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Klonierung der cDNA des Protein A Kinase-Adaptor-Proteins-2 und Untersuchungen zur Regulation seiner mRNA in humanen fötalen Osteoblasten
… erstmalig die vollständige cDNA-Sequenz des Proteinkinase A-Ankerproteins-2 (AKAP-2, 7,5kB) ermittelt. Zu diesem neuen humanen Gen sind bis dato außer einem unvollständigem Datenbankeintrag noch keine experimentellen Daten zur cDNA, mRNA oder zum Protein veröffentlicht. Mittels Northernblot …
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THE ROLE OF OUTER MEMBRANE PROTEIN A IN ANAPLASMA MARGINALE CELLULAR INVASION AND ITS POTENTIAL AS A CROSS-PROTECTIVE ANTIGEN
… identified A. phagocytophilum outer membrane protein A (ApOmpA) as being critical for cellular invasion and is expressed during infection of mammalian but not tick cells. Disrupting ApOmpA-host cell interactions significantly inhibits A. phagocytophilum entry into host cells. ApOmpA and its A. …
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