Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 492 for “"Proteases"”.
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Allosteric activation of periplasmic HtrA proteases
Escherichia coli responds to outer-membrane stress using a signaling cascade initiated when DegS, an HtrA-family periplasmic protease, cleaves RseA, a transmembrane anti-sigma factor, causing downstream release of the [sgima]E sigma factor and transcription of stress-response genes. Each subunit of …
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Modification of soybean proteins by immobilized proteases
Trypsin and alpha-chymotrypsin were immobilized on nylon pellets or porous glass by covalent methods to change molecular properties and functional characteristics of soybean proteins. The amount of trypsin immobilized on nylon pellets using the glutaraldehyde method was high when the pellets were …
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Next generation approaches toward engineering therapeutic proteases
… can be envisioned in which engineered proteases could replace antibodies by irreversibly inactivating a large excess of disease-associated target proteins in a catalytic fashion. Technological advances in molecular biology have made laboratory-based evolution techniques for protein …
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Functional and structural studies of AAA+ proteases
AAA+ proteases are found in all domains of life. They degrade misfolded proteins as well as specific regulatory factors and thus play critical roles in protein quality control and numerous cellular processes. These enzymes share a conserved architecture in which a hexameric AAA+ ATPase recognizes, …
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Assembly of Coagulation Proteases on the Membrane Surface
Overall, this thesis aids in elucidating the importance of the assembly of the various coagulation proteins on the membrane surface.
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Action of Proteases on Rat Liver Lactic Dehydrogenase
Made available in DSpace on 2014-12-05T19:37:20Z (GMT). No. of bitstreams: 1 6001630.pdf: 3698177 bytes, checksum: 8964bace76080bb6ebb40fae8cbc03ee (MD5) Previous issue date: 1960
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Identification and characterisation of proteases in Mycobacterium tuberculosis
… to cause extensive tissue necrosis. Exported proteases are commonly associated with virulence in bacterial pathogens, yet their role in Mycobacterium tuberculosis has virtually not been studied. Preliminary experiments showed M. tuberculosis culture filtrates contained a proteolytic activity …
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Characterization of subtilisin-like serine proteases in trichomonas vaginalis
… worldwide. In this study, subtilisin-like serine proteases (subtilases) were examined for their putative role in cell viability. Published data of other eukaryotic protozoan parasites confirm the importance of subtilases for adhesion and invasion. Serine protease inhibitor assays show that 3,4 DIC …
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Functional relevance of mitochondrial proteases in metabolism and cancer
Durante esta Tesis doctoral, hemos intentado contribuir al estudio de la función mitocondrial y su relación con la patología humana, mediante varias aproximaciones. En primer lugar, hemos generado ratones deficientes en dos proteasas mitocondriales, Omal y Lonp1, para analizar su relevancia …
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Structural and Molecular Basis for Multifunctional Roles of Cellular Proteases
Cellular proteases were first known for their roles in processing peptides such as signal peptides. Recent discoveries have begun to unravel their multifunctional roles in many other biological processes including blood pressure regulation and virus infection. However, the knowledge gathered thus …
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The role of proteases in development of cancer in mice
Mice of the A/J strain are useful models for cancer because they develop tumours spontaneously after treatment with ethylcarbamate. The first study was to investigate the changes in protease activity in tumour bearing and in normal tissues such as lung, liver and skelatal muscle. Using a modified …
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Purification of Two Myosin -Degrading Proteases From Porcine Skeletal Muscle
… yet been clearly described. Two myosin degrading proteases, a serine protease and a cysteine protease that might have some function in myosin degradation, were isolated from porcine skeletal muscle. Activity of the serine protease was inhibited by DFP, aprotinin, benzamidine, soybean trypsin …
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Properties of Two Thermostable Proteases From Streptomyces Rectus Var. Proteolyticus
Made available in DSpace on 2014-12-11T18:16:52Z (GMT). No. of bitstreams: 1 7405531.pdf: 4708979 bytes, checksum: 57ef642ae623065b232857a855803c17 (MD5) Previous issue date: 1973
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Nanoparticle-chaperoned urinary 'synthetic biomarkers' for profiling proteases in cancer
… to tumors whereupon selective cleavage by active proteases releases peptide fragments that are cleared by the renal system into the urine. These peptide fragments are pre-designed with internal photolabile triggers that un-cage isobaric peptide mass tags optimized for multiplexed LC MS/MS …
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Erwinia carotovora extracellular proteases: characterization and role in soft rot
… by phenanthroline, which inhibits Zn-metalloproteases, but not by Ecc intracellular proteins. Analysis of deletion mutants indicated a 1.2 kb region necessary for Prt1 production. Sequencing of the pSK1 insert revealed a 1,041 bp open reading frame (ORF1) corresponding to the prtl region. …
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Coronavirus Proteases as Therapeutic Targets: Development of Biosensors to Detect Inhibition of Protease Activity and Separation of the Multiple Functions of Coronavirus Papain-Like Proteases
… I utilized two approaches targeting the proteases encoded within all coronaviruses. The papain-like protease and 3C-like protease of coronaviruses are responsible for cleaving viral polyproteins early during infection, and this step is required for viral replication. To quantitatively …
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The role of midgut serine proteases in Aedes aegypti vector competence
… through the alimentary tract may exploit serine proteases in the midgut to enhance vector infection. Recent genetic and biochemical experiments have demonstrated that dengue virus type 2 (DENV-2) may require proteolytic processing by midgut trypsins to efficiently infect Aedes aegypti. These …
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Substrate specificity and properties of heat-stable proteases from Pseudomonas fluorescens
Extracellular proteases and lipases from strains P26, 32A, Pl, and P27 of Pseudomonas fluorescens were produced using a dialysis membrane method. The proteases were partially purified by molecular exclusion chromatography (MWC0=60,000) and selected biochemical characteristics were determined. …
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