Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 90 for “"Protease activity"”.
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ANALYSIS OF PROTEASE ACTIVITY USING MEMBRANE- COATED BIOMIMETIC MICROSPHERES
Poisoning by Botulinum Neurotoxins (BoNTs) leads to the severe neuroparalytic illness known as botulism, which is characterized by flaccid paralysis, respiratory failure, and death. BoNTs are the most acutely toxic substances on the planet, so detection at physiologically relevant concentrations is …
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Protease activity sensors for monivasive diagnosis and monitoring of pulmonary diseases
… be to administer a probe that can detect disease activity inside the body and emit a signal to the outside world. To this end, our group has developed "activity-based nanosensors", which detect dysregulated protease activity at the site of disease and release a reporter that can be measured in the …
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Effects of drying and heating conditions on protein denaturation, starch damage and protease activity of corn: Its relationship to wet millability
Laboratory heated and dried yellow dent corn was investigated in three parts for this study. The first part determined the effects of moisture content, heating time, and temperature on starch damage (gelatinization) and protein denaturation of individual corn kernels. For different genotypes, …
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Coronavirus Proteases as Therapeutic Targets: Development of Biosensors to Detect Inhibition of Protease Activity and Separation of the Multiple Functions of Coronavirus Papain-Like Proteases
… I utilized two approaches targeting the proteases encoded within all coronaviruses. The papain-like protease and 3C-like protease of coronaviruses are responsible for cleaving viral polyproteins early during infection, and this step is required for viral replication. To quantitatively …
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Purification and characterization of a novel protease form Burkholderia strain 2.2N
… Agar, indicating the bacterium also produces a protease. Azocoll hydrolysis was used to detect and measure protease activity. Protease activity was two-fold higher at pH of 7.5 than pH 9.0 and 25-fold at pH 4.0. Cultures grown in media containing 1.0 % yeast extract (YE), tryptic soy, tryptone …
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Investigation of the Enzymes Involved in Lantibiotic Biosynthesis: Lacticin 481 and Haloduracin
… is required for achievement of full biological activity. In lacticin 481 maturation, this peptide cleavage reaction is performed by the N-terminal protease domain of the lacticin 481 transporter, LctT. The activity of the protease domain of LctT was reconstituted in vitro and its substrate …
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ΜΕΛΕΤΗ ΤΗΣ ΠΑΡΑΓΩΓΗΣ IGA1 ΠΡΩΤΕΑΣΗΣ ΑΠΟ MYCOPLASMA ΚΑΙ UREAPLASMA ΑΝΘΡΩΠΟΥ ΚΑΙ ΖΩΩΝ
… IMMUNOGLOBULIN AT MUCOSAL SURFACES, IGA PROTEASES PRODUCED BY PATHOGENIC BACTERIA ARE CONSIDERED POTENTIALVIRULENCE FACTORS FOR ORGANISMS THAT CAUSE DISEASE OR GAIN ENTRY AT MUCOUS MEMBRANES. TO DETERMINE THE ROLE OF IGA PROTEASE IN THE PATHOGENICITY OF MYCOPLASMAL DISEASE, A VARIETY OF …
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Influence of various health beneficial spices on some characteristics of yogurt culture bacteria Lactobacillus acidophilus, and sensor acceptability of spicy probiotic yogurt
… with oxgall (bile tolerance) and skim milk (protease activity) with 1% (v/v) of freshly extracted spice juice. Control samples had no spice juice. A probiotic blueberry yogurt was made with 0.05% of individual spice juice. Physico-chemical characteristics of the three bacterial cultures used …
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High throughput microfluidic technologies for cell separation and single-cell analysis
… parameter in single cell study and pericellular protease activity plays a key role in regulating the microenvironment of individual cells. Therefore, this thesis focuses on establishing new methods to address the issues of cell size and single cell protease measurement. We first develop a …
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Insight into the structure and function of empty class II major histocompatibility complexes
… both humans and mice. Immature DC also secrete a protease activity that is capable of generating antigenic peptides from whole antigen. The protease activity secreted by dendritic cells is characterized and the role of empty MHC proteins in dendritic cell antigen presentation is discussed.
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Evaluation of selective γ-secretase inhibitors as novel modulators of TNF-α-mediated apoptosis
… as the catalytic subunits of the γ-secretase protease complexes, the presenilins are responsible for the cleavage of the amyloid precursor protein (APP) and generation of amyloid beta (Aβ). To date, γ-secretase proteases have over 120 substrates which demonstrate contribution to a diverse …
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Effects of stratification on in vitro protein synthesis using components from embryos of Pinus lambertiana Dougl.
… quantity of supernatant protein to produce this activity. The supernatant fraction from stratified embryos was more active than that from dormant embryos but there were indications that this increase was due to seed imbibition. Concentrations of other essential components of the system were the …
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Identification and characterization of a matrix metalloproteinase (Pta1-MMP) expressed during Loblolly pine (Pinus taeda) seed development and germination
… for a seed-expressed MMP exhibits Zn2+-dependent protease activity, and is inhibited by the active site-binding hydroxamate inhibitor GM6001 and EDTA. The Pta1-MMP gene is expressed during embryo development, with transcript levels increasing from proembryo to early cotyledonary stage, then …
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Forensic taphonomy: processes associated with cadaver decomposition in soil
… biomass carbon (Cmic), phosphodiesterase activity, protease activity and soil pH. Microbial activity was triggered within 24 hours of cadaver burial and this initial flush of activity was likely due to both soil-borne and cadaveric microbes. Intermediate Phase decomposition was typically …
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Evasion of RIG-I/MDA5 and TLR3-mediated innate immunity by hepatitis A virus
… adaptor of RIG-I and MDA5, by the viral 3ABC protease precursor. The 3ABC cleavage of MAVS requires both the protease activity of 3Cpro and a transmembrane domain in 3A that directs 3ABC to mitochondria. The signaling ability of MAVS depends on its mitochondrial localization; cleavage of MAVS …
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Identification of HIV protease mutants with improved specificities toward an Alzheimer's Disease associated peptide sequence
We have developed the first protease specificity engineering technology intended to serve as a general-purpose source of target-specific protease mutants with potential therapeutic applications. We used this E. coli-based screening system to isolate multiple HIV protease mutants with improved …
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Molecular approaches to increasing resistance of wheat (Triticum aestivum L.) towards two insect pests; Cereal aphid (Sitobion avenae F.) and Wheat bulb fly (Delia coarctata Fallen).
… and D. coarctata was studied to characterise gut proteases and their inhibition by host plant proteinase inhibitors (PIs). Investigation of proteolytic digestion in S. avenae gut showed that in spite of being a phloem-feeding insect, cereal aphid could digest ingested protein, using cysteine …
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Particle sensors based on amplified quenching of conjugated polymers for biosensing applications
… as potential material platforms to detect protease activity. CP-based particles have greater sensitivity versus CPs in solution due to interchain and intrachain interactions afforded in the solid state. The CP core of the particle can be made using layer-by-layer assembly, a versatile …
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MMP-7 nanosensors for urine-based detection of chemotherapy-induced senescence and pulmonary fibrosis
… a stable cell cycle arrest and pro-inflammatory activity via the secretion of senescence-associated secretory phenotype (SASP). Senescence is considered a fundamental tumour suppression mechanism to prevent the expansion of oncogenically-stressed premalignant cells, including in lung cancer. When …
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