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Showing 1 to 20 of 49 for “"Propidium Iodide"”.

  1. Adaptation of Three Different Apoptotic Methods in Equine Bronchoalveolar Cells and Comparison of Bronchoalveolar Lavage Cell Apoptosis in Normal and COPD Affected Horses Before and After Dexamethasone Administration

    Leichner, Teri LynnVirginia Tech2001

    … 3 methods of measuring apoptosis, Vindelov's propidium iodide with Triton-X (PI/Triton-X), 7-aminoactinomycin D (7-AAD), and Annexin V with propidium iodide (Annexin/PI) were evaluated in equine airway lymphocytes. A significant linear relationship was found for equine bronchoalveolar lavage …

    vt Repository record

  2. The protective role of tumour necrosis factor alpha in the heart

    Meiring, James JustusDivision of Cardiology2002

    … was also evaluated by the measurement of propidium iodide uptake on flow cytometry. Preconditioning and TNFα enhanced cell viability with a reduction in propidium iodide uptake by 28% and 41 % respectively versus the ischaemic controls. To evaluate whether TNFα activation of the nuclear …

    cape-town Repository record

  3. Use of monoploid solanum phureja in cell and tissue culture techniques for potato improvement

    Owen, Henry R.Virginia Tech1987

    … SEPEL yielded two populations of pollen based on propidium iodide staining of DNA. These populations corresponded to pollen separation based on size parameters alone, introducing the potential for flow sorting of pollen to increase seed set in 4x-2x crosses to tetraploid cultivars. Protoplast …

    vt Repository record

  4. Function Study of Atrial Fibrillation-Linked Connexin Mutants and Heterotypic Docking Compatibility of Cardiac Connexins

    Ye, Willy GThe University of Western Ontario2015

    … expressing L221I or V85I alone showed increased propidium iodide uptake, which was also the case for cells co-expressing L221I and wildtype Cx40, suggesting increased hemichannel opening probability. A novel AF-linked Cx45 mutant (M235L) was found to impair GJ function. These alterations in …

    uwo Repository record

  5. Cell Toxicology Study of RRR-Alpha-Tocopheryl Polyethylene Glycol 1000 Succinate (TPGS).

    Muenyi, Clarisse Sornsay2005

    … employing the nucleic acid-binding fluorophore; propidium iodide. A colorimetric 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyl tetrazolium bromide (MTT) assay was used to evaluate cell viability. Cell death can occur through necrosis or apoptosis. Our results suggested that TPGS triggered apoptotic …

    etsu Repository record

  6. The effects of some antibiotics and lipopolysaccharide on oxidative burst and phagocytosis of neutrophils

    Böhmer, Reinhard HansiDivision of Medical Microbiology1990

    … was modified for single laser systems by using propidium iodide labeled Staphylococcus aureus (S.aureus) as a quantitative measure of phagocytosis and simultaneously the green fluorescence of oxidized 2'7' dichlorofluorescein diacetate was used to measure oxidative burst. Propidium-iodide labels …

    cape-town Repository record

  7. The effect of doxorubicin on the U2OS cell cycle

    Weston, Katherine DMassachusetts Institute of Technology2008

    … analysis with Bro mo-2-deoxyuridine (BrdU) and Propidium Iodide (PI) staining. The cells are unable to recover from the severity of this damage and become apoptotic. When a 2 [mu]M dose is applied to the cells, a G2 arrest occurs. This is shown by lower levels of Cyclin B in the G2 phase during …

    mit Repository record

  8. Development of a Flow Cytometry Method for the Identification and Enumeration of Enterococcus faecalis

    Magbitang, Riza2025

    … of the cells. Additionally, the application of propidium iodide (i.e., a viability stain), afforded the method the ability to differentiate between cells with intact and compromised cell membranes, which raises the potential of quantifying cells that are in viable-but-non-culturable (VBNC) …

    usm Repository record

  9. Development of quantitative methods for quality assessment of islets of Langerhans

    Pisania, AnnaMassachusetts Institute of Technology2007

    … dyes, fluorescein diacetate (FDA) and propidium iodide (PI). These methods are operator- dependent and prone to error due to the variability in islet size and shape and are not predictive of transplantation outcome. We developed quantitative assays that allowed reproducible evaluation …

    mit Repository record

  10. Engineering the Optical Properties of Gold Nanostructures for Biomedical Applications

    Au, Leslie2013

    … cancer cells using flow cytometry coupled with propidium iodide staining. In addition to Au nanocages and nanoframes, I have synthesized Au microplates using a biological macromolecule, bovine serum albumin: BSA), as the reducing agent. I exposed the reductive hydroxyl groups in the protein by …

    wustl Repository record

  11. Organ and primary culture of medaka (Oryzias latipes) testis: Test systems for the analysis of cell proliferation and differentiation

    Song, MiyeounTechnische Universität Dresden

    … as determined by BrdU labeling, and CFSE and propidium iodide PI labeling. Based on cytological criteria, the proliferating cells were mostly spermatogonia and possibly also preleptotene spermatocytes. Differentiation of spermatocytes into spermatids or spermatozoa was also observed, mainly …

    qucosa-diss

  12. Gingko biloba extracts protect auditory hair cells from cisplatin-induced ototoxicity by inhibiting perturbation of gap junctional intercellular communication

    최, 성준2014

    … then analyzed by immunocytochemistry (Annexin V/ propidium iodide) and Western blots. The function of GJIC was evaluated by scrape-loading dye transfer (SLDT). Basal turn organ of Corti (oC) explants from neonatal (p3) rats were exposed to cisplatin (1–10μM) and EGb (50–400μg/ml). The number of …

    ajou Repository record

  13. Phospholipase A2 Induced Monocyte Chemotaxis to Apoptotic Cells

    Karikari, Kwasi2014

    … and monitored through annexin-V binding and propidium iodide(PI) staining followed by flow cytometric analyses. THP-1 monocytes are employed in chemotaxis assay with monocyte chemotactic protein (MCP-1) as a positive control. The preliminary data show equivalent group IIa sPLA2 association …

    vcu Repository record

  14. Reconstitution of a Multi-layered, Differentiated Cornea by HTERT-Immortalized Corneal Epithelial Cells Transduced with Thymidine Kinase Transplanted onto Denuded Mouse Corneas

    Kalangara, Jerry P.2011

    … Immunofluorescence (IF) using Anti-Laminin and Propidium iodide (PI) was used to assess presence of basement membrane after epithelial removal. Cultured HyTK cells were stained with Cell Tracker Green CMFDA (5-chloromethylfluorescein diacetate) and transplanted onto the right eye after …

    utswmed Repository record

  15. Angiotensin II-induced Apoptosis in Vascular Smooth Muscle Cells: Differential Effects in Human Saphenous Vein and Human Internal Mammary Artery

    Gritzuk, Michael MarkCreighton University2006

    … Apoptosis was measured using annexin V-FITC and propidium iodide followed by flow cytometric analysis. Protein expression of Bax, Bcl-2, and phospho-p38 MAP kinase was examined by Western Blot. The data revealed three major findings: (1) Angiotensin II induced significant apoptosis in VSMCs of …

    creighton Repository record

  16. Effects Of Cholesterol On Bovine Sperm Survival During Cooling and Freezing

    Bach, Kathryn2009

    … Percent viability was evaluated post-thaw by propidium iodide (PI) and SYBR 14 staining using the LIVE/DEAD Sperm Viability Kit protocol. For the study using high cholesterol yolk, domestic hens were fed one of three diets supplemented with 0.5%, 1.0%, or no cholesterol. Eggs from these hens …

    cornell Repository record

  17. Midaesin - a histone H2A antimicrobial peptide from the South African abalone, Haliotis midae

    French, LeeDepartment of Molecular and Cell Biology2021

    … employing the membrane impermeable dye propidium iodide, revealed membrane disruption of V. anguillarum cells exposed to 13 µM or 30 µM of the Midaesin-containing peptides for 30 minutes, respectively. Secondary structure analysis by circular dichroism indicated a shift in the secondary …

    cape-town Repository record

  18. Detergent effects on disinfectant susceptibility of Escherichia coli and listeria monocytogenes attached to stainless steel

    Walton, JulieUniversity of Wolverhampton2012

    … type. Flow cytometry using the fluorochrome propidium iodide (PI) revealed significant increases in cell membrane permeability of both organisms by all detergents except sodium dodecyl sulphate (SDS) and the effect was much greater in E. coli. Increasing above the in-use concentration of SAS …

    wlv Repository record

  19. Enhancing the Industrial Potential of Filamentous Cyanobacteria

    Johnson, Tylor J.2016

    … 9) was accurate, the non-viable cell stain (propidium iodide) also bound to viable cells. Additional non-viable cell stains were evaluated, but none were accurate at quantifying viability. Thus we concluded that the viable cell stain SYTO® 9 is a reliable assay and can be used in …

    sdstate Repository record

  20. Nanosecond Pulsed Electric Fields Induce a Mitochondria-Independent Apoptosis in B16F10 Melanoma Cells <i>In Vitro</i>

    Ford, Wentia Elissa2019

    … of additional apoptotic markers Annexin V and propidium iodide revealed no phosphatidylserine externalization and no direct electric field effects on membrane permeability, respectively. However, characteristic apoptotic morphological features of plasma membrane blebbing and F-actin …

    odu Repository record

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