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Showing 1 to 20 of 22 for “"Prophages"”.
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Transfer of Transducing Prophages in Bacterial Matings
Made available in DSpace on 2014-12-05T21:45:36Z (GMT). No. of bitstreams: 1 6202883.pdf: 3421068 bytes, checksum: b3875701df65ce35f4f3b91f900bdf18 (MD5) Previous issue date: 1962
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The ecology of prophages at the microscale
… Through a large-scale genomic survey of prophages and their hosts across the bacterial domain, I estimated the extent to which prophages co-opt quorum sensing systems by encoding their components as auxiliary genes. Collectively, this work suggests that variability in community assembly …
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Characterization of novel prophages in lysogenic murine isolate lactobacillus murinus EF-1
… Bioinformatic analyses identified 3 intact prophages within the L. murinus EF-1 genome of 26.1, 25.4, and 49.6Kbp size. To confirm phage existence, the antibiotic induced lysate was subject to transmission electron microscopy. An identified Siphoviridae morphology was confirmed with an 80nm …
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The effect of active prophage carriage on the virulence of Pseudomonas aeruginosa
… phages integrated into the LES genome as active prophages, which provide fitness advantages, but can also be induced to their replicative form and destroy their host by lysis. Active phages are very common in the CF lung and infecting P. aeruginosa strains commonly carry many prophages, but …
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Bacteriophages in the honey bee gut and amphibian skin microbiomes: investigating the interactions between phages and their bacterial hosts
… contributions that phages, and particularly prophages, provide to the bacterial members of two separate host-associated communities: the honey bee (Apis mellifera) gut microbiome and the amphibian skin microbiome. My second chapter surveyed publicly available whole genome sequences of common …
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Extracellular Contractile Injection Systems (eCIS) - Characterization of Conserved Phage Tail-like Structures in Streptomyces
… emphasized by the discovery of rare eCIS-like prophages that are the likely evolutionary link between the two. I classify eCIS into six distinct groups and show that group 1 eCIS are ubiquitous in the Streptomyces genus, where their production is linked to the developmental program of these …
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Biological Consequences of Atypical Phage Conversion in Gram-Positive Pathogens
… screening that uncovers the presence of episomal prophages in a number of S. aureus clinical isolates. QPCR characterization of one of these strains, MSSA476, reveals that the episomal nature of one of its prophages, ɸSa4ms, would have been missed if sequencing whole genomic and not specifically …
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Physiological roles and regulation of the cryptic prophage-encoded small protein DicB and small RNA DicF in Escherichia coli
… coli K12. Bacterial genomes harbor cryptic prophages that have lost genes required for induction, excision from host chromosomes, or production of phage progeny. DicB and DicF are encoded on the dicBF operon, which is located in the immunity region of the cryptic prophage Qin. DicB and DicF …
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Hypermutation and adaptation of experimentally evolved marine Vibrio bacteria
… prophage excision. Members of this family of prophages are found in other proteobacteria, including well-studied human pathogens, from very different environments. Mutators are known to arise spontaneously in wild and clinical bacteria, but the extent of their adaptive contribution is unknown. …
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Quorum sensing in the genus Hafnia - in vitro and in silico investigations
… silico approach to interrogate the landscape of prophages within the genus Hafnia. The review of literature regarding QS highlighted the need to disambiguate which strains merely possess QS genes from those that express them, and moreover to understand the environmental contexts influencing their …
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Development of a Genetic Modification System in <i>Clostridium scatologenes</i> ATCC 25775 for Generation of Mutants
… using environmental sources. To induce prophages, cultures of <i>Clostridium scatologenes</i> ATCC 25775 were exposed to an effective concentration of mitomycin C at 2μg/ml and 5μg/ml. Induction with temperature was performed at 42ºC and 55ºC. Bacteriophage liberation, determined by a …
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Characterising vaginal Lactobacillus strains from young South African women with persistently optimal vaginal microbiota – Developing the framework for an African vaginal probiotic product development platform for reproductive health
… and VFDB), the presence of integrated prophages using VirSorter and phage defence mechanisms using the DefenseFinder tool. Lastly, putative bacteriocins were identified using BAGEL4. Results: The most common community state type (CST) identified across all participants and visits was …
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Phenotypic and genetic diversity of pseudomonads associated with the roots of field-grown canola
… plasmids, 50% (N=33) contained inducible prophages, 24% (N=16) contained integrons and 23% (N=15) contained transposons. Examination of the MGEs associated with a subset of rhizosphere pseudomonads revealed that MGEs were present in the isolates independent of the degree of similarity …
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Ultraviolet Induction Properties of SALMONELLA POTSDAM Bacteriophages
… a single prophage each and SP9 contained two prophages P9a and P9c. The ultraviolet light sensitivities of each of these lysogenic strains and the indicator strain SP2 were also examined.</p> <p>All three lysogenic strains were found to be inducible. SP3 was induced an average of 3.59% and SP4 …
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Characterizing the genotypic and phenotypic diversity of Gardnerella vaginalis from vaginal clinical samples
… (91%) and augmentin (100%). The presence of prophages in G. vaginalis isolates was also investigated, revealing the presence of bacteriophage (phage)-like particles that could not be classified into any known phage families, whose phage status remains to be confirmed. In conclusion, G. …
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Metagenomic Analyses of the Human Gut Virome
… human gut symbionts, seven of which harbored 10 prophages, one of which: Bacteroides cellulosilyticusWH2) was represented by a library of >25,000 isogenic transposon mutants covering 80% of genes in its genome. Once assembled, this model microbiota was subjected to a staged phage attack with a …
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Genetic analysis of bacteriophages from clinical and environmental samples
… assignable as novel phages or uncharacterised prophages, the next largest assignable group being single-stranded eukaryotic virus genomes. Sputum from a cystic fibrosis patient contained DNA typical of phages of bacteria that are traditionally involved in CF lung infections and other bacteria …
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Characterisation of the temperate bacteriophages of salmonella enterica and salmonella bongori
… has been suggested that a variable assortment of prophages provides a transferable repertoire of pathogenic determinants in Salmonella. The temperate phages present in Salmonella enterica serovar Typhimurium isolates have been most extensively investigated to date. However, how variable is the …
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Characterisation of vaginal Prevotella strains from a cohort of South African adolescent girls and young women with and without bacterial women with and without bacterial vaginosis
… virulence genes, as well as for the presence of prophages or anti-phage defence mechanisms. Phylogenetic analysis by cpn60 gene sequencing and core genome alignment revealed that intra-sample diversity was generally low, but at least one sample had two distinct strains at the same time point. The …
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Proteome, transcriptome and metabolome plasticity in closely related strains of Escherichia coli - K12 and its diversity during molecular evolution processes
… are due to insertion sequences, defective prophages and two sites due to the W3110 inversion between the ribosomal RNA genes rrnD and rrnE. The rate of nucleotide changes between both the strains is estimated to be relatively low with almost identical genome structures. Hence the following …
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