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Showing 1 to 16 of 16 for “"Pronase"”.
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A Probe Into Escherichia Coli Ribosomal Protein Topography Using Pronase
Made available in DSpace on 2015-05-14T14:58:49Z (GMT). No. of bitstreams: 2 license.txt: 4848 bytes, checksum: 96035ab3f5e1c23cc7138a224ce498bd (MD5) 7511542.PDF: 4582358 bytes, checksum: 1a07f54b1911962035b01e235e2f8038 (MD5) Previous issue date: 1974
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The isolation, partial purification and preliminary characterization of a growth factor from chick embryo brains
… while Sf-AMP had no effect. Upon treatment with pronase, the biological activity of fraction P6-3 increased; this increase was nearly 30% greater than what would be expected from a simple additive effect of any mitogenic activity of pronase alone together with P6-3 alone. This may suggest the …
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Host-Parasite Interrelations: Bacterial Adherence to and Invasion of Epithelial Cells
… degrees. HeLa cell glycopeptides, generated by pronase digestion of chloroform:methanol-extracted cells, were tested for their ability to inhibit binding by P.IIa to Western blots of HeLa cell macromolecules. HeLa cell extracts inhibited P.IIa binding prior to pronase treatment, but inhibitory …
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Variability of Cholesterol Accessibility in Human Red Blood Cells Measured Using a Bacterial Cholesterol Binding Toxin
… membranes and with plasma triglyceride levels. Pronase treatment of RBCs only modestly altered cholesterol accessibility. Plasma from various healthy individuals differentially modulated RBC cholesterol accessibility. Individuals on hemodialysis, who have an unexplained increase in …
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Macrophage Suppression By a Low Molecular Weight Fraction of Murine Spleen Cell Culture Supernatant
… six months. MSF is resistant to treatment with Pronase E, but is, however, sensitive to acid hydrolysis. Activity of MSF in spleen cell culture supernatants from normal mice does not differ from supernatants from mice immunized with L. monocytogenes. It was therefore concluded that MSF is not …
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An In Vivo Perfusion Apparatus for Studies on Gingival Disease : Assessment by a Perfusion of Nonspecific Proteolytic Enzymes
… animal for four days at the same rate. A 1% pronase solution was perfused for four days on one animal at the rate of .96 ml/day and another animal at 4.8 ml/day. The appropriate control solutions were delivered on the contralateral side of each animal undergoing enzyme perfusion. Data …
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Post-translational modifications of SEL24K from salmon eggs and ZPA from Xenopus laevis eggs
… separated through SDS-PAGE. A nonspecific in-gel pronase digestion was performed on the excised band of ZPA to produce informative small glycopeptides. Lectin affinity chromatography was used for the enrichment of these glycopeptides. An in-gel PNGase F digestion was also carried out to release …
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Mechanisms of cytokinin action
… of the nuclei enhanced 6BA binding by 100% while pronase treatment reduced binding. Binding sites liberated by DNase treatment showed reduced affinity for 3BA. It is suggested that nuclei contain cytokinin receptor site of a protein nature.
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Chemical and enzymatic modification of whey proteins
… chymotrypsin, papain, bromelin, pepsin and pronase. Efficiency of the hydrolytic process was evaluated by monitoring parameters such as temperature, pH, substrate concentration, enzyme to substrate ratio and ionic composition. Correlation of the enzymes' mode of action on whey proteins to …
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Control of sex myoblast migration in C. elegans
… of larval cuticles and subsequent pronase E digestion. My method efficiently isolates both low- and high-abundance cell types from all larval stages. This technical advance will not only facilitate studies such as regulation of actin dynamics with high-resolution microscopy, but is …
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Untersuchungen zur Sorption von Innenraum-Luftschadstoffen durch Wolle
… the fibre proteins through the enzymatical alloy Pronase.
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Effect of high and low dosage of fresh and frozen semen on accessory sperm number, fertility and embryo quality in artificially inseminated cattle
… embryos/ova after partial digestion with Pronase followed by compression of the embryo/ovum with a cover slip. From 129 inseminations to normally cycling cows, 98 embryos/ova were recovered. To reduce male effects, embryos/ova used were randomly balanced across treatments, by ejaculate …
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Discovery of Novel Tubulin Polymerization Inhibitors and Survivin Inhibitors as Potential Anticancer Agents
… and also protected survivin from digestion by pronase in the DARTS assay. The most potent compound from the in vitro assay effectively suppressed tumor growth in melanoma xenograft. This novel scaffold represents a new structure template for survivin inhibitors and can be further developed as …
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Interference of Toxoplasma gondii with IFN-γ-regulated gene expression of its host cell
… ist teilweise sensitiv gegen Behandlung mit Pronase, was auf die Involvierung eines Proteins hinweist. Experimente mit Parasiten, die von ihren Wirtszellen durch eine semipermeable Membran getrennt inkubiert wurden und die trotzdem die IFN-γ-induzierten MHC Klasse II Expression der …
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Studies on the Complement Receptor of a Human Lymphoblastoid Cell Line
… was cleaved by trypsin, chymotrypsin, papain and pronase, leading to the appearance of a new cell-associated moiety with a molecular weight of 22,000 daltons. These studies have established the clonal character of the C3d receptor, and provided homogeneous populations of cells bearing a receptor …
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Συγκριτική ανοσοϊστοχημική μελέτη υποδοχέων οιστρογόνων καρκίνου μαστού σε τομές κρυοστάτη και παραφίνης προς βιοχημικές και ανοσοενζυματικές μεθόδους
The monoclonal antibody ER-ICA (HSΡΓ-222) to human estrogen receptor protein amplified via the peroxidase-antiperoxidase (P.A.P.) method was used to detect the ER in cryostat, paraffin and acetone sections. In 72 of the 86 studied primary breast cancers, a comparative evaluation was done between …