Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 232 for “"Promoter region"”.
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Functional characterization and analysis of Carica papaya MYB24 promoter region in Arabidopsis
This Thesis was approved for publication on 2019-07-18 at 16:35.
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The functional significance of the G to A point mutation in the promoter region of the Apolipoprotein AI gene
AG to A transition at position -76 in the promoter region of the apoAI gene was previously identified, and the A-76 has been shown to be associated with high apoAI levels. The functional significance of the point mutation was assessed by analysing the DNA-protein binding and promoter activities of …
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An investigation of genome-wide promoter region cytosine-phosphate-guanine (CpG) Island methylation profiles in patients with chronic hepatitis B virus infection
… viral DNA that could be adjacent host promoter Cytosine-phosphateGuanine (CpG) islands. This would activate or silence genes including tumour suppressors and result in the clinical disease phenotypes of hepatic inflammation, fibrosis and HCC that characterise chronic HBV infection. …
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Investigation of DNA methylation at the promoter region of the aralkylamine N-acetyltransferase (AANAT ) gene in South African children with Autism Spectrum Disorder
… Diagnostic Observation Schedule (ADOS-2). The promoter region and gene body of AANAT was sequenced (case n=26, control n=26) and DNAme analysis was performed with the Epityper massARRAY system (case n=19, control n=20). Urinary 6-hydroxymelatonin sulphate (6-OHMS) was quantified with an …
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Temporal examination of DNA methylation profile reprogramming in the promoter region of PGC-1α during the progression of insulin resistance and type 2 diabetes mellitus in rodent models
… in the form of DNA methylation, in the promoter region of peroxisome proliferator activated receptor gamma coactivator 1 alpha (PGC-1α), a known regulator of mitochondrial biogenesis. Following the initiation of a high fat diet, we observed significant genotypic (DNA methylation) and …
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Integration and Excision Mechanism of Bacteroides Conjugative Transposons
… shows that RteB binds to 168bp upstream of rteC promoter region. However, the mutant did not bind to any probes tested. Footprint assay is required to determine the exact binding site on rteC promoter region.
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DNA methylation analysis and identification of a novel antisense transcript in the Peg3 imprinted domain
… gene 3) imprinted domain. We investigated this region for both CpG islands and long antisense transcripts, two common features of imprinted gene clusters. First, we performed a systematic survey of DNA methylation status of the CpG islands in this region of the mouse, cow, and human genomes. We …
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Effect of Autoregulated TxeR on the Expression of <I>Clostridium difficile</I> Toxins
… expression from the toxA, toxB, and txeR promoters, gene fusions were constructed that contained the various promoter regions and a reporter gene. The immunodominant region of toxin A located at the carboxy-terminus, termed the repeating units (ARU), was selected as the reporter gene. …
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Effects of arsenic and UVB radiation on induction of cyclin D1 in human keratinocytes.
… indicates that both arsenic and UVB affect promoter activity in various ways, including methylation. We undertook to examine the effects of arsenite and UVB on activation of the cyclin D1 promoter related to changes in promoter methylation. While data had been inconclusive in identifying …
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Determining the Roles that DICER1 and Noncoding RNAs Play in Endometrial Tumorigenesis
… tumorigenesis. Aberrant methylation of the promoter region of the MLH1 DNA mismatch repair gene in endometrial cancer is associated with loss of MLH1 expression and a "mutator phenotype" in endometrial and other cancers. The molecular and cellular processes leading to aberrant methylation of …
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Identification of a Minimal Cis-element and Cognate Trans-factors Required for the Regulation of Rac2 Gene Expression during K562 Cell Differentiation
… of the Rac2 gene is mediated by the Rac2 promoter region. Nested 5’- deletions of the promoter region identified a critical regulatory region between -4223 bp and -4008 bp upstream of the transcription start site. Super shift and chromatin immunoprecipitation assays indicated binding by …
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DNA methylation of the clusterin promoter: Associations with Alzheimer’s Disease risk and related phenotypes
… was to investigate methylation within the CLU promoter region in peripheral blood samples. Specifically, to determine whether there is a relationship between specific methylation sites and AD-related phenotypes. The first study aimed to crosssectionally assess CLU promoter region methylation …
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Investigation of Transcriptional Regulation of 5'-Nucleotidase in Dictyostelium Discoideum
… codon) for the 5nt expression. The 5nt promoter region was cloned and analyzed to investigate the expression of 5nt. Analysis of the cloned 5nt promoter fused to lacZ enabled the localization of the 5nt expression in pstAB cells during development. To identify cis-acting regulatory …
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In situ Lokalisierung, PGPR-Effekt und Regulation des ipdC-Gens der Azospirillum brasilense Stämme Sp7 und Sp245 bei verschiedenen Weizensorten, sowie endophytische Kolonisierung durch Herbaspirillum sp. N3
… were tested to generate a fusion of the ipdC promoter with a gfp or rfp reporter gene. Constructing a translational promoter fusion with the gfp variant mut3 on plasmid level made expression analyses possible. With this method the promoter region of strain Sp7 located directly upstream of the …
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Studies on the Regulation of Acetyl-CoA Carboxylase and its Influence on Bacillus subtilis Differentiation
… of Spo0A to a cis-element (0A box) in the promoter region of the operon has recently been identified as a strict requirement for biofilm formation and sporulation, two widely-studied cell differentiation processes which occur after the exponential growth phase. Here, we present evidence to …
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Ενεργοποίηση του ογκογονιδίου ha-ras1 του ανθρώπου από ογκογονίδια ιών και κυττάρων
… ON THE REGULATION OF THE HUMAN H-RAS1 GENE PROMOTER. THE RESULTS CAN BE SUMMARIZED AS FOLLOWS: THE MUTANT T24 H-RAS1 PROMOTER REGION (FROM A HUMAN BLADDER CARCINOMA) BEHAVES AS A STRONGER PROMOTER THAN THE PROMOTER OF THE NORMAL H-RAS1 GENE. IN CELL LINES WHICH EXPRESS THE MIDDLE T ANTIGEN …
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Analysis of FY Promoter and Hepatocystis Load in South African Vervet Monkeys (Chlorocebus Aethiops)
… in humans, and the FY*0 (FY Null) allele in the promoter of this gene results in the absence of this receptor on the exterior of the cell (Miller et al., 1977; Miller et al., 1975; Miller et al., 1976; Barnwell et al., 1989; Perkins and Schall, 2002; Martinsen et al., 2008; Tung et al., 2009). …
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The characterization of the ABF-1 promoter
… In these studies, the mapping of the ABF-1 promoter and the critical 5' regulatory elements that control ABF-1 gene expression were analyzed through 5' deletional analysis. 5' -DNA flanking pieces of the promoter region were created through PCR and inserted into a promoterless cloning vector …
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role of NF-<i>k</i>B signaling in multiple myeloma
… from Multiple Myeloma Cell Line JJN3, and BMF promoter region was PCR amplified and cloned into pCR 2.1 TOPO vector using One Shot® Chemically Competent E. coli. After confirmation of the clone through polymerase chain reaction (PCR) and restriction analysis, BMF promoter (2.5 Kb) was released …
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The effect of maternal nutrition on offspring gene regulation via epigenetic modulation
… histone H3 at lysine 4 residues within the p16 promoter region. I also found maternal protein restriction down-regulate p21 gene expression in the mammary gland of rat offspring. p21 is a key factor responsible for tumor suppressor p53-dependent cell cycle arrest and has been accepted as a …
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