Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 12 of 12 for “"Promoter interactions"”.
-
Investigating the chromatin dynamics that underlie CRISPRi-validated enhancer-promoter interactions
… with the previous understanding of enhancer-promoter interactions (EPIs), raising questions regarding the mechanism by which EPIs are produced. Then, this thesis aims to investigate the ways by which chromatin dynamics enable the function of CRISRPi-validated EPIs, in order to explain the …
-
The interplay of global chromosomal organisation, promoter-enhancer interactions and transcription
… is transcription, which starts at the core promoter region. While core promoters are crucial for transcriptional initiation, they are insufficient for establishing complex tissue- and condition-specific gene expression patterns in multicellular organisms. Additional transcriptional control …
-
Transcriptional regulation and genome structure
… DNA regulatory elements known as enhancers and promoters. This leads to the formation of a DNA loop connecting the enhancer and the promoter resulting in the subsequent transcription of the gene. Thus the structuring of the genome into DNA loops is important in the control of gene expression. …
-
Investigating the function of DNA G-quadruplex structures in human enhancers
… potential in diverse genomes, including promoters and enhancers. Enhancers interact with target promoters through 3D folding of interphase chromosomes to regulate gene transcription. However, the functional relevance of G4 structures at non- coding enhancer elements remains poorly …
-
Regulatory Landscapes Associated With Changes In Nuclear Architecture
… Chromatin is organised hierarchically, with interactions between regulatory regions such as enhancers and promoters being hosted within megabase-scale domains. Enhancer-promoter alterations have been mostly described in differentiation scenarios, while a number of studies concluded that such …
-
Non-Parametric Analyses of the Regulatory Roles of LINE-1 Retrotransposons during Motor Neuron Differentiation
… regions may participate in chromatin interactions with cis-regulatory sequences in a cell-type specific manner analogous to enhancer-promoter interactions. Conclusion: The thesis elucidates a set of functional hypotheses concerning putative regulatory roles of repetitive elements, L1 …
-
Molecular Interactions of Type III Secretion System Transcriptional Regulators in Pseudomonas aeruginosa: ExsA and ExsD
… by interfering with ExsA dimerization and promoter interactions. Upon host cell contact, ExsD is sequestered by the T3SS chaperone ExsC, resulting in the release of ExsA and an up-regulation of the T3SS. ExsA is an AraC/XylS-type transcriptional regulator and belongs to a subfamily of …
-
Integrative analysis of transcriptional activity and genome architecture changes upon viral infections
… functional terms. ATAC-Seq uncovered proximal promoter regions (PPR) that showed an over-representation of specific transcription factor binding sites in each of the clusters. These correlated well with the annotated functions of the associated clusters. Further, I found that lytic mCMV …
-
Characterization of the vaccinia virus ankyrin repeat protein K1 on NF-κB inhibition and its contributions to viral pathogenesis
… RelA (p65) subunit of NF-κB. Moreover, p65-CBP interactions were blocked in the presence of K1. However, K1 did not preclude NF-κB binding to oligonucleotides containing B binding sites. This implies that NF-κB-promoter interactions still occurred in the presence of K1, but NF-κB cannot properly …
-
Algorithms for analyzing complex structural variations in cancer genomes
… We have also discovered that a large portion of promoter-promoter interactions, detected by ChIA-PET, are found to be formed by distal genomic regions linked to be adjacent by somatic translocations in MCF-7 genome, showing that phased SVs analysis by Weaver has enabled the analysis of …
-
DNA topology and Pol II CTD phosphorylation as stepwise regulators of chromatin architecture in differentiating human stem cells
… of its C-terminal domain (CTD), which modulates interactions with co-transcriptional regulators and condensates. These mechanisms likely intersect: phosphorylated Pol II may be recruited to negatively supercoiled or secondary structure-forming sites, while negative supercoiling can also act as a …
-
Regulatory architecture of the Pax6 locus
… the mechanism and specificity of cis-regulatory interactions. As well as addressing whether the DNA looping model for regulatory interactions applies to the mouse Pax6 locus, I will identify which elements facilitate such interactions and if they vary between cell types. Utilising ChIP-array …