Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 6 of 6 for “"Prokaryotic DNA"”.
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Kupplung der DNA-Methyltransferase M.SssI mit Triplehelix-bildenden Oligodesoxynucleotiden
DNA modifying enzymes, like DNA methyltransferases (DNA MTases) and restriction endonucleases (REases), could in principle be used to map or manipulate genomes. However, these enzymes are inappropriate for that purpose because of their generally short recognition sequences. An increase of their …
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Exploration of Microbial Genomic Sequences via Comparative Analysis
… identifying likely protein coding sequences in prokaryotic DNA by combining comparative analysis of DNA sequences with more common noncomparative methods. In the comparative component of the analysis, regions of DNA are aligned with related sequences from the DNA databases; if the translation of …
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THE IDENTIFICATION OF NOVEL INORGANIC CARBON SENSITIVE ENZYMES
… homology domain comprises the majority of prokaryotic and eukaryotic adenylyl cyclases, and has been further divided into 4 sub-Classes (a-d) based on active site polymorphisms. A number of Class IIIb adenylyl cyclases display elevated catalytic activity in the presence of inorganic carbon. …
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The Functional Characterization of the LysR-Type Transcriptional Regulator QseD and the SorC-Type Transcriptional Regulator LsrR in Enterohemorrhagic Escherichia coli
… of E. coli. LTTRs, the largest known family of prokaryotic DNA binding proteins, contain two functional domains, an N-terminal helix-turn-helix (HTH) and a C-terminal co-factor binding domain which allows for oligomerization [5]. We have demonstrated that QseD indirectly represses transcription …
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Super-specific DNA methylation by a DNA methyltransferase coupled with a triple helix-forming oligonucleotide
In this work, an engineered variant of the DNA cytosine-C5 methyltransferase (MTase) M.SssI has been conjugated with a triple helix-forming oligonucleotide (TFO) and the properties of the enzyme-TFO conjugate were evaluated using a variety of biochemical and biophysical methods. M.SssI is the only …
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The Gut Microbiome: Is Fecal Matter Microbial Composition a Proxy for Intestinal Microbial Composition in Studies of the Microbiome
… is done by collecting and sequencing the DNA of the microbiome of the fecal matter from model organisms or human subjects. Studies that use this method of sample collection and analysis assume that fecal matter microbiomes are similar to intestinal microbiomes, and that it can be used as a …