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Showing 1 to 15 of 15 for “"Primordial germ cells (PGCs)"”.
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Integrin signalling in pluripotent cells acts as a gatekeeper of mouse germline entry
Primordial germ cells (PGCs) are the undifferentiated precursors of gametes and the sole mechanism by which genetic information is transmitted across generations through the germline. They appear at the proximal posterior region of the mouse embryo, where the somatic germ layers begin to emerge and …
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Downregulation of reproductive homeobox gene 6 (RHOX6) interferes with male germ cell differentiation
… a member of the Rhox family, is expressed in primordial germ cells (PGCs) in the developing gonad and the placenta, although the biological function of Rhox6 in determination of PGCs and placental cells remains unknown. To investigate the Rhox6 function, we generated male chimeras by injection …
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Molecular analysis of germ cell specification
… survival is the correct differentiation of primordial germ cells (PGCs). PGCs are the first cells in the germline, and their correct specification is essential for the eventual production of gametes (eggs and sperm). Murine PGCs arise as a small population in the post-implantation epiblast …
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Mechanisms of Mouse Primordial Germ Cell Migration: The Role of SDF-1 and Cytoskeletal Dynamics
Primordial germ cells (PGCs) are the earliest embryonic precursors to the gametes: spermatozoa and oocytes. In many species, PGCs must migrate to the embryonic gonad during early development in order to produce functional gametes. Failure to reach the gonad increases the risk of tumorigenesis, …
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The identification of novel factors required for the development of primordial germ cells
… from arising. Mutations that occur in somatic cells can lead to disease, which may be detrimental to that single individual. However, germ line mutations can be passed on to the next generation. These mutations not only drive genome evolution but can also impair the fitness of the offspring. It …
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Role of small RNAs and chromatin in transposable element silencing during global demethylation
… despite being a more static mark in somatic cells. Global hypomethylation is a hallmark of epigenetic reprogramming in mammalian primordial germ cells (PGCs), the early embryo and in naïve embryonic stem cells (ESCs). Genome integrity is crucial during early development, as the germline DNA …
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EXPERIMENTAL DISENGAGEMENT OF MORPHOGENETIC PROCESSES AND ITS EFFECTS ON PRIMORDIAL GERM CELL COLONIZATION OF THE GONADAL RIDGES IN RANA PIPIENS
During normal embryogenesis in Rana pipiens the primordial germ cells (PGCs) undergo a displacement from deep within the ventral endoderm of the Stage 17 tailbud embryo to the dorsal endodermal crest of the hatched Stage 20 larva; eventually they exit the endoderm and populate the paired gonadal …
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DNA crosslink repair safeguards genomic stability during pre-meiotic germ cell development
Germ cells are single-handedly responsible for the transmission of genetic and epigenetic information (the instructions to make new life) from one generation to the next. It is therefore, of paramount importance that germ cells maintain the stability of their genome. Failure to do so can result in …
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DNA Translesion Synthesis Factors are Essential for Mammalian Embryonic Germ Cell Development
Germ cells are responsible for the transmission of genetic and epigenetic information between generations and hence alterations to the genome can have effects across generations. Faithful propagation of the genome is therefore of unique importance as mutations in the germline are the source of …
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Understanding antigen processing in chickens using genome editing technology
… are expressed at high levels in haemopoietic cells. One hypothesis is that each MHCII B gene co-evolves with one of the two DMB genes, resulting in differential expression, but the interactions and importance of each DM gene are unknown. In this thesis, the genome editing approach of clustered …
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Responses to transposon activity and genome damage during germline development
The germline is the cell lineage that is responsible for the inheritance of genetic information in multicellular eukaryotes. In most animal species, germ cells are set aside from somatic lineages during early embryonic development and follow a unique developmental programme that culminates in the …
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Signalling and transcriptional regulation of human primordial germ cell specification
Primordial germ cells (PGCs), the precursors of gametes, are specified in early post-implantation human embryos, which are inaccessible for direct investigation. This process can however be modelled using human embryonic stem cells (hESCs) for in vitro derivation of human PGC-like cells (hPGCLCs). …
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Genesis of the human germline
… thesis was to examine the specification of human primordial germ cells (PGCs) and the mechanism regulating the transient gain and loss of competence for germ cell fate. Specification of PGCs in humans occurs during weeks 2 to 3 in gastrulating embryos, which eventually develop into sperm and ova, …
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Spatial Transcriptome Profiling of Gastrulating Primate Embryos
… postimplantation development, the pluripotent cells of the embryonic disc, the embryo proper, undergo gastrulation. This key process establishes the three germ layers, endoderm, mesoderm and ectoderm, which set the basis for all tissues emerging in the human embryo. Since postimplantation human …
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The susceptibility of primordial germ cells to malignant transformation and isolation and characterization of members of a new gene family differentially expressed in invasive and non-invasive immortalized male germ cells
In dieser Arbeit untersuchten wir die Potenz der Primordial Keimzellen (PGCs) zur malignen Transformation unter Verwendung eines transgenen Mausmodell. In diesem Modell verwendeten wir das SV40 large Tumor Antigen (LTA),welcher mit dem Keimzellen alkalische Phosphatase (GCAP) Promotor verbunden …