Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 37 for “"Primordial germ cells"”.
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Idenficiation of the zebrafish primordial germ cells
Thesis (Ph. D.)--Massachusetts Institute of Technology, Dept. of Biology, 1997.
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The identification of novel factors required for the development of primordial germ cells
… from arising. Mutations that occur in somatic cells can lead to disease, which may be detrimental to that single individual. However, germ line mutations can be passed on to the next generation. These mutations not only drive genome evolution but can also impair the fitness of the offspring. It …
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The susceptibility of primordial germ cells to malignant transformation and isolation and characterization of members of a new gene family differentially expressed in invasive and non-invasive immortalized male germ cells
In dieser Arbeit untersuchten wir die Potenz der Primordial Keimzellen (PGCs) zur malignen Transformation unter Verwendung eines transgenen Mausmodell. In diesem Modell verwendeten wir das SV40 large Tumor Antigen (LTA),welcher mit dem Keimzellen alkalische Phosphatase (GCAP) Promotor verbunden …
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Germline development in Platynereis dumerilii and its connection to embryonic patterning
… unified theory about the evolutionary origin of germ cell specification still requires detailed analyses in other species. In this thesis, I present evidence on the establishment of the germline and its correlation with pluripotency and embryonic patterning in the polychaete Platynereis …
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The role of mir-290-295 in murine embryonic and germ cell development
… specifically in early embryos and embryonic germ cells. This thesis examines the in vivo consequences of targeted deletion of mir-290-295 in the developing mouse embryo. Mir-290-295 deficiency results in a partially penetrant embryonic lethality. Mir-290-295 is not required for early …
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Oct4 and Sox2 regulate methylation at the Igf2/H19 imprinting control region
… required for transgene hypomethylation in F9 cells and that Oct4 was present at the endogenous ICR. In addition, each of the octamers is flanked by a sequence that allowed cooperative binding of Oct4 and Sox2 in vitro. To determine at which developmental stage the octamers regulate ICR …
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Integrin signalling in pluripotent cells acts as a gatekeeper of mouse germline entry
Primordial germ cells (PGCs) are the undifferentiated precursors of gametes and the sole mechanism by which genetic information is transmitted across generations through the germline. They appear at the proximal posterior region of the mouse embryo, where the somatic germ layers begin to emerge and …
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The Regulation of UHRF1 during Mammalian Embryogenesis
… gametes, and secondly during the development of primordial germ cells. Although some of these changes in DNA methylation are correlated with gene expression, it is not yet understood why this epigenetic reprogramming is so extensive. Recent research has revealed that multiple different examples …
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Exposures and the Impacts on the Epigenome that Persist from Youth to Adulthood and Intergenerationally
… reprogramming in the developing embryo and primordial germ cells, causing dysregulated methylation and the onset of diseases into adulthood. This work is the first of its kind to show the intergenerational effects of in utero arsenic exposure on adulthood disease phenotypes and the inherited …
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Downregulation of reproductive homeobox gene 6 (RHOX6) interferes with male germ cell differentiation
… a member of the Rhox family, is expressed in primordial germ cells (PGCs) in the developing gonad and the placenta, although the biological function of Rhox6 in determination of PGCs and placental cells remains unknown. To investigate the Rhox6 function, we generated male chimeras by injection …
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Oogenesis of Ostertagia circumcincta, a parasitic nematode of the abomasum of sheep
… the changes observed in the development of the germ cells from the primordial stage through the first cleavage division. 1. The primordial germ cells arise at the tip of the germ tube which lies just posterior to the oesophagus. 2. The period of growth following each stage in the development of …
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Regulation of amnion induction during the human formative pluripotency transition
… to differentiate to all somatic lineages and primordial germ cells. hPSC exists in distinct phases—naïve, formative, and primed—each with differing developmental potential. Naïve hPSC resembles the pre-implantation epiblast, retain trophectoderm (TE) potential, but are resistant to somatic …
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Molecular analysis of germ cell specification
… survival is the correct differentiation of primordial germ cells (PGCs). PGCs are the first cells in the germline, and their correct specification is essential for the eventual production of gametes (eggs and sperm). Murine PGCs arise as a small population in the post-implantation epiblast …
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Mechanisms of Mouse Primordial Germ Cell Migration: The Role of SDF-1 and Cytoskeletal Dynamics
Primordial germ cells (PGCs) are the earliest embryonic precursors to the gametes: spermatozoa and oocytes. In many species, PGCs must migrate to the embryonic gonad during early development in order to produce functional gametes. Failure to reach the gonad increases the risk of tumorigenesis, …
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Role of small RNAs and chromatin in transposable element silencing during global demethylation
… despite being a more static mark in somatic cells. Global hypomethylation is a hallmark of epigenetic reprogramming in mammalian primordial germ cells (PGCs), the early embryo and in naïve embryonic stem cells (ESCs). Genome integrity is crucial during early development, as the germline DNA …
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EXPERIMENTAL DISENGAGEMENT OF MORPHOGENETIC PROCESSES AND ITS EFFECTS ON PRIMORDIAL GERM CELL COLONIZATION OF THE GONADAL RIDGES IN RANA PIPIENS
During normal embryogenesis in Rana pipiens the primordial germ cells (PGCs) undergo a displacement from deep within the ventral endoderm of the Stage 17 tailbud embryo to the dorsal endodermal crest of the hatched Stage 20 larva; eventually they exit the endoderm and populate the paired gonadal …
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Insights Into Mitochondrial Genetic and Morphologic Dynamics Gained by Stochastic Simulation
MtDNA mutations in mammalian cells are implicated in cellular ageing and encephalomyopathies, although mechanisms involved are not completely understood. The mitochondrial genetic bottleneck has puzzled biologists for a long time. Approximate models of genetic bottleneck proposed in the literature …
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DNA crosslink repair safeguards genomic stability during pre-meiotic germ cell development
Germ cells are single-handedly responsible for the transmission of genetic and epigenetic information (the instructions to make new life) from one generation to the next. It is therefore, of paramount importance that germ cells maintain the stability of their genome. Failure to do so can result in …
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DNA Translesion Synthesis Factors are Essential for Mammalian Embryonic Germ Cell Development
Germ cells are responsible for the transmission of genetic and epigenetic information between generations and hence alterations to the genome can have effects across generations. Faithful propagation of the genome is therefore of unique importance as mutations in the germline are the source of …
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