Global ETD Search
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Showing 1 to 15 of 15 for “"Primordial germ cell"”.
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Molecular Mechanisms in Primordial Germ Cell Development in Zebrafish
In vielen Organismen, Primordiale Keimzellen, werden in frühen Entwicklungsstadien, entfernt von der Region wo sich die Gonaden entwickeln werden, spezifiziert. Daher müssen die Zellen zu dieser Region migrieren, wo sie sich dann in Spermien bzw. Oozyten differenzieren. Eine allgemeine Eigenschaft …
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Signalling and transcriptional regulation of human primordial germ cell specification
Primordial germ cells (PGCs), the precursors of gametes, are specified in early post-implantation human embryos, which are inaccessible for direct investigation. This process can however be modelled using human embryonic stem cells (hESCs) for in vitro derivation of human PGC-like cells (hPGCLCs). …
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Mechanisms of Mouse Primordial Germ Cell Migration: The Role of SDF-1 and Cytoskeletal Dynamics
Primordial germ cells (PGCs) are the earliest embryonic precursors to the gametes: spermatozoa and oocytes. In many species, PGCs must migrate to the embryonic gonad during early development in order to produce functional gametes. Failure to reach the gonad increases the risk of tumorigenesis, …
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EXPERIMENTAL DISENGAGEMENT OF MORPHOGENETIC PROCESSES AND ITS EFFECTS ON PRIMORDIAL GERM CELL COLONIZATION OF THE GONADAL RIDGES IN RANA PIPIENS
During normal embryogenesis in Rana pipiens the primordial germ cells (PGCs) undergo a displacement from deep within the ventral endoderm of the Stage 17 tailbud embryo to the dorsal endodermal crest of the hatched Stage 20 larva; eventually they exit the endoderm and populate the paired gonadal …
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MicroRNAs in early embryonic development : dissecting the role of miR-290 through miR-295 in the mouse
… expressed during early embryonic and germ cell development. This miRNA family is conserved only among mammals. miR-290 cluster miRNAs are transcribed and processed from a common capped and polyadenylated primary transcript in the mouse. Deletion of the miR-290 cluster in the mouse …
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Biological Confinement of Zebrafish Using RNAi
… The deadend (dnd) gene is required for primordial germ cell (PGC) development and survival. Previous studies have shown that zebrafish dnd knockouts develop into sterile adults without disrupting somatic development. In efforts to induce sterility in zebrafish, short hairpin RNA (shRNA) …
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Using Advanced In Vitro Models to Investigate the Adverse Effects of Methotrexate on the Developing Epigenome
… in vitro. Treatment of mouse embryonic stem cells (mESC) and epiblast-like cells with low-dose MTX, as a proxy for exposure in early embryonic development, resulted in a significant downward trend in the global average of CpG methylation, as well as hypomethylation and hypermethylation of …
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Illuminating the Black Box: Defining and Modeling Peri-Implantation Human Embryogenesis
… is a complex process where the single cell zygote transitions to the multilineage blastocyst, comprised of the epiblast (precursor of the fetus), hypoblast (precursor of the yolk sac) and trophoblast (precursor of the placenta). The blastocyst then implants into the maternal uterus and …
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Adamts9 is critical for the development of primary ovarian follicles
… type 1 motif, member 9) is an extracellular matrix metalloprotease that is highly evolutionarily conserved and critical for development in vertebrates. Knockouts of ADAMTS9 in mice and AdamTS-A in Drosophila melanogaster are embryonic lethal; and abnormal ADAMTS9 activity has also …
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Utilising embryonic and extra-embryonic stem cells to model early mammalian embryogenesis in vitro
… uterus, the mouse embryo is comprised of three cell lineages: first, the embryonic epiblast (EPI) that forms the embryo proper, second, the extra-embryonic ectoderm (ExE) which contributes to the foetal portion of the placenta, and third, the visceral endoderm (VE) that contributes to the yolk …
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Single-cell genomics in rabbit and mouse elucidate eutherian embryonic development
… accessibility profiles of almost 180,000 single cells and high-resolution histology sections from embryos spanning gastrulation, implantation, amniogenesis, and early organogenesis. Using a novel computational pipeline, we compare the transcriptional landscape of rabbit and mouse at the scale of …
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A Translational Approach to Modeling Unique Aspects of Germ Cell Development During Self-Organization of the Primate Embryo
<p>The specification of germ cells during embryonic development is vital not only for the development of an organism, but quite literally for the survival and propagation of its entire species. Recent work has demonstrated that several aspects of human primordial germ cell (hPGC) development are …
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Using human genomics to decipher biological mechanisms underlying reproductive ageing and fertility in women
… This provides direct evidence that female germline mutation rate is heritable and highlights a mechanism for maternal effects on offspring health. Chapter 4 extends the exome sequence analysis to an extreme form of early menopause, i.e. POI, which is often considered a monogenic disorder, …
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Responses to transposon activity and genome damage during germline development
The germline is the cell lineage that is responsible for the inheritance of genetic information in multicellular eukaryotes. In most animal species, germ cells are set aside from somatic lineages during early embryonic development and follow a unique developmental programme that culminates in the …