Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 14 of 14 for “"Primitive Endoderm"”.
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Development of an Interferon Bioassay and Primitive Endoderm Cell Lines to Study Lineage Specification During Early Bovine Embryogenesis
… specification events, trophectoderm (TE) and primitive endoderm (PrE) specification, occurring between days 6 and 8 of gestation. The TE is responsible for uterine attachment and production of interferon-tau (IFNT), the factor of maternal recognition of pregnancy in ruminants. The PrE forms …
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Manipulating Embryonic Development and Endometrial Function in Ruminants
… extraembryonic lineages. One such lineage is the primitive endoderm, which will eventually give rise to the yolk sac. Once believed to be a vestigial structure, the yolk sac is now believed to play a more prominent role in embryogenesis as it provides nutrients to the preimplantation embryo. The …
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A Study of Mouse Peri-Implantation Morphogenesis
… extraembryonic lineages, the trophectoderm and primitive endoderm. My first goal was to characterise the blastocyst to egg cylinder transition in mouse embryos and understand its mechanism through live imaging and inhibitor treatments (1). Then, I aimed to examine how the interplay between the …
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The Role of Mga in the Survival of Pluripotent Cells During Peri-implantation Development
… though the number of differentiated primitive endoderm cells remained initially stable. Despite the loss of pluripotent cells, there is no change in the pattern of expression of Nanog or Oct4, pluripotent cell markers, or Gata4, a primitive endoderm marker. Expression of Ornithine …
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Modelling Physical Mechanisms Driving Tissue Self-Organisation in the Early Mammalian Embryo
… structure with two distinct tissue layers - the primitive endoderm and epiblast. It was originally assumed that cells differentiated to form these layers in situ, but more recent evidence suggests that both cell types arise scattered throughout the inner cell mass, and it is thus proposed that …
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The Role of NADPH Oxidase in ROS Mediated Differentiation
Mouse teratocarcinoma F9 cells differentiate into primitive endoderm (PrE) when treated with retinoic acid (RA) or H2O2 and these changes are accompanied by an upregulation of Wnt6 and activation of the canonical WNT/β-catenin pathway. Data from our lab shows PrE differentiation is accompanied by …
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Hyperosmotic Stress Enzyme Signaling Modulates Oct4, Nanog, And Rex1 Expression And Induces Prioritized Differentiation Of Murine Embryonic Stem Cells
… lineages, upregulating</p> <p>markers of primitive endoderm and suppressing epiblast markers.</p> <p>The studies were continued in the peri-implantation model, embryoid bodies</p> <p>(EBs), in which differentiation is allowed rather than actively suppressed. Unstressed</p> <p>EB culture …
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Directed differentiation of endodermal cells from mouse embryonic stem cells
… an attempt has been made to produce definitive endoderm and subsequently cells that can serve as pancreatic and hepatocyte precursors from mouse embryonic stem cells. By using retinoic acid and basic-FGF, in the absence of embryoid body formation, mouse embryonic stem cells were differentiated …
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Extracellular Signalling and Stem Cell Self-Renewal
… (FGF) signalling triggers the differentiation of primitive endoderm (PrE) cells fated to contribute to the yolk sac, while cells unreceptive to FGF form the epiblast (Epi) that subsequently contributes to the embryo proper. In vitro, FGF signalling is required for preimplantation Epi-derived mouse …
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The Role of Snail Homolog 1 in Embryonic Stem Cell Differentiation
… transition: EMT) as they ingress through the primitive streak and form mesoderm. To better understand the molecular pathways of EMT during this developmental transition, we developed a model system utilizing mouse embryonic stem: ES) cells. We show that EMT occurs during ES cell …
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Supplementing Bovine Embryo Culture Media to Improve the Production and Quality of In Vitro Produced Bovine Embryos
… also had increased transcripts of hypoblast/primitive endoderm (PE) markers. These results indicate that IL6 may improve pregnancy retention of IVP embryos by improving yolk sac development, but further work is needed to confirm this theory. Activation of STAT3 by IL6 could be blocked with a …
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Mechanisms behind the fate of early chromosomal and transcriptional heterogeneities in the mouse embryo
… which will form the fetus, the extra-embryonic primitive endoderm and the outer layer of the extra-embryonic trophectoderm (TE). This organization prepares the embryo for implantation and subsequent development. This study aims to explore two broad questions: 1) what mechanisms dictate the fate …
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Cell Fate Decisions in the Early Mammalian Embryo
… as the blastocyst, consisting of the epiblast, primitive endoderm and trophectoderm, which will give rise to the new organism, the yolk sac and placenta respectively. These three lineages must be established from the totipotent zygote via two successive cell fate decisions in the appropriate …
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Methods for Dissecting High Dimensional Single Cell RNA Sequencing Data
… expression dynamics during the formation of primitive endoderm cells. Preliminary results from human embryo staining are used to validate the findings from FFAVES. In summary, I hope to demonstrate that ES and FFAVES serve as powerful tools for increasing the amount of information that can be …