Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 6 of 6 for “"Primer extension assay"”.
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Association between Chronic Hepatitis B Virus Infection and Interleukin-10, Tumor Necrosis Factor-α Gene Promoter Polymorphisms
… TNF-α gene promoter (-308, -238) by single base primer extension assay. The frequency of C/C genotype at position -592 of IL-10 gene promoter was higher in the HBV clearance group than that in the persistence group in univariate analysis (12.7% vs. 7.5%, p=0.036). IL-10 gene promoter -592 C/C …
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Using <i>Drosophila melanogaster</i> as a Whole-Model Animal System to Elucidate the Mechanism of Action of Novel Anticancer Agents
… and react with DNA to form a bulky DNA lesion. A primer extension assay was carried out to visualize the DNA damage occurring on a 392-nucleotide PCR product in response to the agents. It was determined modification can occur at three out of four DNA bases. In order to test the agents and …
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Structural and chemical studies of RNA as anti-cancer target and signaling molecule
… in cancer. Telomerase catalyses telomere extension by using a portion of its RNA as a template. During this process a RNA/DNA heteroduplex is formed. We have attempted to inhibit telomerase by targeting this heteroduplex using nucleic acid binders such as intercalators. In this …
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Surface enhanced Raman scattering based assays for DNA detection by
… scattering (SERS) based molecular diagnostic assays for the detection of specific DNA sequences have been developed in recent years to compete with the more common fluorescence based approaches. Current SERS assays require either time consuming separation steps that increase assay cost and can …
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Y-family DNA polymerase architecture: three structural features control accurate deoxy CTP insertion opposite N2-deoxy-guanine-benzo-a-pyrene
… large openings that accommodate the BP moiety. Primer extension assays with purified proteins show that DNAP IV correctly inserts dCTP opposite BP more than 10-fold faster than it mis-inserts dATP, dGTP, or dTTP. In contrast, h/V/Dpo4-class DNAPs have small active site openings, which cannot …
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The molecular biology of calbindin-D₉
… ends) with rat and bovine cDNA sequence-derived primers for amplification. The full length of these sequences was confirmed by primer extension assay. The human cDNA includes a coding region for 79 amino acids,57 nucleotides 5'- and 159 nucleotides 3'-non-coding region, and a poly(A+) tail. …