Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 10 of 10 for “"Primer Template"”.
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[RNA polymerase ribozymes]
… synthesizing RNA using the information in an RNA template. The goal of the present work is to provide experimental evidence in support of this conjecture, by isolating such ribozymes in the laboratory. We created a large pool of RNA molecules each containing a previously isolated RNA ligase …
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New insights into the mechanism of dna replication on unmodified and benzo[a]pyrene modified templates using surface plasmon resonance
… an effort to actively seek a correctly pairing template-dNTP for catalysis. Polymerases have evolved to efficiently utilize this method of dNTP selection to speed up dNTP selection. </p> <p> Bulky DNA adducts such as benzo[a]pyrene interfere with replication by forming structures within the …
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RNA replication by poliovirus RNA-dependent RNA polymerase: effects of residue 5 on elongation complex stability and processivity
… a single nucleotide into an oligonucleotide primer. The polymerase must first go through multiple steps of initiation before processive replication can happen. During primer-dependent initiation, the polymerase must bind the primer/template RNA substrate and undergo a conformational change to …
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Investigation Into The Binding Interactions Of Klenow Fragment To Dna Modified With Carcinogens Af And Aaf Using Surface Plasmon Resonance
… It is possible that the bulky, mismatched primer template terminus rearranges the active site allowing the modified dG to rotate and serve as a template for the incoming dCTP. SPR is a useful label free technique to determine both equilibrium binding constants (KD), and dissociation rates …
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Molecular and Biochemical Analyses of a Three-Subunit Euryarchaeal Clamp Loader Complex
… loader, which installs the sliding clamp onto primer-template junction of DNA in an ATP-dependent manner. The critical clamp loader acts as molecular switch from distributive to processive DNA synthesis. Here, we describe a novel form of clamp loader from the euryarchaeon Methanosarcina …
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Structure-Substrate Binding Relationships of HIV-1 Reverse Transcriptase
… on both poly(rA)(dT) and a random base RNA template hybridized with a DNA oligomer showed that p51 significantly affects the enzyme activity. The increase in processivity by p51 in the p66/p51 heterodimer was also demonstrated. These observations suggested that the integrity of p51 is …
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Single-molecule and ensemble studies of DNA replication system
… activity for an AAA+ protein that can act as a template for the assembly of another protein complex. In the second study, by applying fluorescence resonance energy transfer (FRET) to the archaeal replication, we reconstituted part of replication machine, up to four different protein components, …
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The structural basis of RNA-catalyzed RNA polymerization
… interactions that recognize and bind the primer-template duplex and those that position the reaction electrophile. It furthermore revealed functional groups that compose the active site. Biochemical evidence and the position of these groups lead us to propose a reaction mechanism similar …
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DNA structural selectivity of binding by the Pol I DNA polymerases from Escherichia coli and Thermus aquaticus
… DNA structures: single-stranded DNA (ss-DNA), primer-template DNA (pt-DNA), and blunt-end double-stranded DNA (ds-DNA) show that the binding selectivity pattern is similar when examined across a wide range of salt concentration, but can differ significantly at any individual salt concentration. …
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Replication-associated base excision repair Of oxidized bases in the mammalian genome
… in the replicating genome where repair of both template strands is equally important because an unrepaired base lesion in either strand could induce mutations. Thus, specific involvement of NEIL1 with the DNA replication machinery may be required to effectively and efficiently accomplish this. …