Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 12 of 12 for “"Primer Pair"”.
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Molecular marker analysis of a segregating monoploid potato family
… sequence repeat analysis with eight polymorphic primer pairs was used successfully to identify the homozygous diploid plants that were added to the monoploids. In total 34 monoploid plants and 14 homozugous diploids were obtained. The degree of heterozygosity revealed by SSR analysis indicated …
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Molecular analysis of algal communities in the San Joaquin River
… water samples. Polymerase Chain Reaction (PCR) primers were designed to amplify the 18S rRNA gene of certain taxonomic groups of freshwater algae; there was limited success in specific amplification. Additionally, a primer pair utilizing both the 16S plastid gene and the 16S rRNA gene was tested …
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Diagnostics and epidemiology of <i>Phytophthora infestans</i> : the cause of late blight of potato
… was developed. A <i>P. infestans</i> specific primer pair (INF FW2 and INF REV) was designed by comparing the aligned sequences of internal transcribed spacer regions (ITS) of a range of <i>Phytophthora </i>species. Following PCR amplification with primers INF FW2 and INF REV, a 613 bp product …
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Phenotypic and Molecular Characterization of the Beetle Pathogens Paenibacillus popilliae and Paenibacillus lentimorbus
… the paraspore in all of the isolates. One primer pair, CryBP2, detected the <I>cry18Aa1</I> gene in 17 of the 21 <I>P. popilliae</I> strains and in all ten of the <I>P. lentimorbus</I> strains. The second primer pair, CryBP4, detected the parasporal gene in 20 of the 21 <I>P. popilliae</I> …
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Mixed Strain Identification of Porcine Reproductive and Respiratory Syndrome Virus in Multiplexed Samples using Nanopore Sequencing
… sequencer. We have developed a novel primer set designed to span Open Reading Frames 3 through 7 of the PRRSV genome, which has allowed for multiplexing of samples, thus reducing individual cost of testing, while yielding significantly more information than previously available. This …
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Assessment of genetic variations within and between populations of Marama Bean (Tylosema Esculentum (Burchell) Schreiber) based on Microsatellites (SSRS) and Intergenic spacer length variation markers in the Namibian germplasm
… In the present study two to four alleles per primer pair were amplified from the 332 marama individuals among 11 populations. The study revealed that substantial genetic variation exists in marama populations and most of this variation (66.5) occurred within populations. The direct-count …
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Determination of soybean cultivar resistance to soybean cyst nematode with quantitative polymerase chain reaction
… Santa Ana, CA)). For the qPCR assay, primer pair for single copy gene HgSNO, which codes for a protein involved in the production of vitamin B6, was selected for H. glycines DNA amplification within soybean roots. In the second experiment, compatible Lee 74, incompatible Peking and …
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Assessing Arbuscular Mycorrhizal Fungal community composition and diversity across a land-use gradient in Eastern Washington prairies
… samples, was amplified using the AMF-specific primer pair NS31-AML2 and sequenced on the Illumina MiSeq platform using 300bp paired-end sequencing. Sequences were quality filtered and grouped into amplicon sequence variants (ASVs) using QIIME2. Taxonomy for the recovered AMF lineages was …
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Determination of the frequency of four pathogenic variants causing inborn errors of metabolism in the western cape black population, using a multiplexed arms pcr approach
… most of the disease burden. Methods: ARMS PCR primers were designed to detect the four variants of interest. Individual PCR methods were optimised for each primer pair, followed by an attempt to combine these reactions in a multiplex assay. A multiplex ARMS PCR method designed to detect both …
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Rhizosphere/Soil Microorganisms in the Phytoremediation of Biphenyl and Dioxane
… conditions only. The fungus was identified by a primer pair ITS4 and ITS1F as Pseudallescheria boydii. The microbial community in the rhizosphere of the poplar and willow trees was sampled from different soil locations in the test-plot over a 5-year period. The denaturing gradient gel …
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Understanding host, pathogen, environment dynamics in the marine environment with a particular focus on the Pacific oyster crassostrea gigas and its pathogens
… used to assess the sensitivity of a range of primer pairs available for detection of OsHV-1 µVar outside of the normal host the oyster. The primer pairs OHVA/OHVB and PCR method were consistently the most sensitive method for the detection of OsHV-1 µVar in all sample types. The response of …
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MAPK and pro-inflammatory mediators in the walls of brain blood vessels following cerebral ischemia
… the core [12]. Cells in the penumbra are impaired and cannot function due to compromised metabolism, but do not die immediately and have therefore become a prime target for neuroprotective treatments [13-15]. A number of neurochemical and pathophysiological events are triggered within the …