Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 8 of 8 for “"Primed Pluripotency"”.
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Identification of the ERK5 Kinase as a Key Regulator of Embryonic Stem Cell Pluripotency
… types in the adult body, a phenomenon known as pluripotency. Distinct pluripotent states have emerged as a key concept in ESC biology; the naïve state safeguards selfrenewal whilst primed pluripotency provides a window for differentiation. However, the molecular mechanisms that control …
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Dissecting Pluripotency and Mammalian Embryonic Development via Droplet Microfluidics
Pluripotency, the ability of a cell to differentiate towards any type of somatic cell is a transient feature of the developing embryo. In vitro, pluripotency can be captured in the form of embryonic stem cells (ESCs). In this study, I developed a microfluidic-based system to encapsulate ESCs into …
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A study into the biology of mESCs exiting naïve pluripotency and technology development for subcellular proteomics
… embryology for decades. One of those aspects is pluripotency, the ability of embryonic cells of certain developmental stages to divide indefinitely without the loss of their potential to differentiate into derivatives of the three primary germ layers. Pluripotency has been captured in vitro by …
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Elucidating the Role of Plakoglobin in Embryonic Stem Cell Culture
Pluripotency – the ability of cells to differentiate into all three germ layers – is of great interest, particularly in the fields of developmental biology and medicine. The in vivo continuum can be separated into three in vitro states that are also found in the embryo: naïve, formative, and primed …
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Defining the essential regulators of naive human pluripotent stem cell reprogramming
… (PSCs) exist in two distinct states – naïve and primed, which are interconvertible. Naïve PSCs uniquely harbour several desirable properties that primed PSCs do not, including their ability to model human pre-implantation biology and developmentally-regulated epigenetic phenomena in vitro. Naïve …
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Exploring functional significance of asymmetric DNA methylation in early mammalian development
… The distribution of mCH, both in ESC naïve and primed pluripotency states, is very heterogeneous, while its nuclear distribution is very homogenous. mCH is physically recognised by a number of pluripotency factors such as Oct4 and Sox2, as well as by other DNA modification-sensitive proteins …
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Pluripotency and patterning in nonhuman primate embryogenesis
… to unravel primate-specific features of pluripotency. This thesis examines common marmoset (Callithrix jacchus) development to explore the molecular cues that govern (1) naïve pluripotency in the preimplantation epiblast, and (2) primed pluripotency in the embryonic disc (EmDisc) in the …
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CHARACTERISING THE REPROGRAMMING DYNAMICS BETWEEN HUMAN PLURIPOTENT STATES
… stem cells (hPSCs) exist in multiple states of pluripotency, broadly categorised as naïve and primed states. These provide an important model to investigate the earliest stages of human embryonic development. Naïve cells can be obtained through primed-to-naïve reprogramming; however, there are …