Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 11 of 11 for “"Prime Editing"”.
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Engineering structural variants with prime editing
CRISPR/Cas-based gene editing has revolutionized biological research by enabling programmable and scalable manipulation of genomic sequences. Prime editing has emerged as a powerful tool to introduce virtually any type of edit with high precision, but editing efficiency varies between edit types. …
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CRISPR AS A TOOL FOR TARGET DISCOVERY AND TECHNOLOGICAL INNOVATION: HIGH-THROUGHPUT CBE SCREENING TO EVALUATE M6A MODIFICATION PATHWAY IN CANCER PROLIFERATION AND DRUG RESISTANCE, AND INNOVATIVE PRIME EDITING STRATEGIES FOR DISEASE MODELLING AND THERAPY
… Short Palindromic Repeats (CRISPR) Cytosine base editing (CBE) screening to perform saturating mutagenesis of m6A-pathway effectors. In details, we assessed the effect of single nucleotide variants (SNVs) in CRC proliferation, highlighting domains of m6A effectors that are critical for promoting, …
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A broadened HLA ligandome uncovers new immunotherapy targets for pancreatic cancer + A prime editor mouse to model a broad spectrum of somatic mutations
… engineered mouse model (GEMM) for performing prime editing in vivo. This system represents a rapid alternative to traditional cancer mouse models, which often take months or years to develop. Through a Creinducible prime editor enzyme encoded in the mouse germline, prime editor GEMMs can …
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Understanding Genomes Through Engineered Structural Variation
… genome engineering technologies such as CRISPR prime editing. I leveraged the ability of prime editors to insert recognition sequences for recombinases at high throughput to engineer genomes at an unprecedented scale. In the process, I made discoveries about the biology of genome engineering, …
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Computational and experimental methods for CRISPR-based saturation mutagenesis screens
… in gene regulation. Second, we developed PrimeDesign to facilitate the rapid design of prime editing (PE) guide RNAs and demonstrate its utility by using recommended designs to install pathogenic variants in human cells. Complementing PrimeDesign, we developed pegPool as a high-throughput …
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Developing Adeno-Associated Viral Vectors As A Gene Editing Platform
… AAV into a platform for CRISPR/Cas9 gene editing. Yet, AAV vector behaviors that are critical for gene editing have been unexplored. To safely and effectively combine AAV and CRISPR/Cas9 for in vivo applications, a better understanding of the potential sites for gene editing and the …
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Predicting protein-DNA interactions using statistical modeling and biophysical representations of high-throughput sequencing data
… and DNA. Second, we identify conditions in which prime editor, a ribonucleoprotein that can target and edit DNA sites, can operate with greater success. Prime editing is done in several steps involving RNA-DNA binding, DNA nickage, reverse transcription, and DNA repair. Numerous factors have to be …
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DEFINING THE FUNCTION OF RICE FLORIGENIC PROTEINS DURING THE REPRODUCTIVE TRANSITION AND INFLORESCENCE DEVELOPMENT
… modulating the function of FT-L1 using the Prime Editing technique, introducing an aminoacidic conversion important for the switch of the florigenic into an anti-florigenic function. My study also highlights FT-L1’s involvement in flower fertility regulation. FT-L1 transcripts and proteins …
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Precision Gene Editing for Muscle Diseases
… effect on the afflicted individual. Gene editing tools like base editors and prime editors enable us to target and correct the underlying genetic causes of these diseases. Base editors enable precise base pair transition edits, while prime editors offer even more versatility, allowing for …
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INTEGRATED TRANSCRIPTOMIC AND TRANSLATIONAL PROFILING OF EIF6-SBDS IN SHWACHMAN-DIAMOND SYNDROM REVEALS DIFFERENT CELLULAR STATES SHAPED BY RIBOSOME COMPOSITION AND FIDELITY
… and interacts with SBDS depletion. Using Prime Editing, we generated isogenic HEK293T lines carrying a N106S allele in heterozygosis and established matched SBDS-depleted models via shRNAi. Polysome profiling, RNA-seq, and Ribo-seq were used to assess how each perturbation influences …
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CRISPR-based functional genomics to study gene regulatory architecture and consequences of genetic variation
… In the third chapter, I introduce a CRISPR prime editing based method that allows for the identification of drug resistance mutations in a multiplex and scaled manner. In the fourth and final chapter, I describe how I envision this work advancing further in order to gain a more comprehensive …