Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 31 for “"Primary cilia"”.
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Characterisation of primary cilia in human hypothalamic neurons
Primary cilia are sensory organelles that protrude from the plasma membrane of most mammalian cells, including neurons. Among their roles, they are recognised to be involved in the control of feeding and energy expenditure by the hypothalamus. However, how cilia exert their role as signalling hubs …
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Investigating the role of primary cilia loss in intrahepatic cholangiocarcinoma
Primary cilia (PC) are important sensory organelles which protrude from the epithelial cells lining bile ducts (cholangiocytes), where they serve as sensors of bile composition, flow and osmolality. PC malformation has been reported in cholangiocarcinoma (CCA), a neoplasia that originates from …
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TTBK2 and Primary Cilia are Required for Purkinje Cell Survival
<p>Primary cilia are a small microtubule based signaling organelle. Primary cilia can be found on almost every mammalian cell, including neurons and glia. Tau Tubulin Kinase 2 (TTBK2) is a critical regulator of the building of primary cilia, and mutations within Ttbk2 cause the adult-onset, …
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The Roles of Primary Cilia in Cardiovascular Diseases and Cancer
<p>Primary cilia are sensory organelles present in most mammalian cell types and regulate cell cycle and signaling pathways. Biochemical and molecular dysfunctions of primary cilia are associated with a wide range of diseases, including cancer, ciliopathies polycystic kidney disease (PKD, liver …
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Structural characterization of primary cilia using accelerated piezoelectrically driven STED nanoscopy
Primary cilia are non-motile, hair-like projections occurring on most mammalian cell types. They play essential roles in transduction of chemical and mechanical signals across the cell membrane. For example, primary cilia are able to transduce sonic hedgehog signals, necessary in embryonic …
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Targeting Primary Cilia Immune Receptor Proteins for the Treatment of Polycystic Kidney Disease Mechanisms
<p><strong>Background: </strong>Primary cilia are cellular organelles project from the cell surface of mammalian cell and play important roles in vertebrate development, organogenesis, health, and others genetic diseases. Primary cilium functions as a mechano-sensor and chemo-sensor. Defect in …
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Stiffness controls primary cilia position and length: mechanisms and potential in reversing osteoarthritis phenotypes
… mechanosensitivity through the action of, the primary cilium. Mechanical stress and the stiffness of the extracellular matrix are key drivers of tissue development and homeostasis. Aberrant mechanosensation is associated with a wide range of pathologies, including diseases such as …
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Prenatal SMN-dependent defects in translation uncover reversible primary cilia phenotypes in a mouse model of spinal muscular atrophy
… I show the involvement in processes related to primary cilia. Assessments of primary cilia in the CNS in vivo and in primary neuronal cultures in vitro confirmed the presence of a primary cilia phenotype in SMA. Finally, to demonstrate that this observed novel phenotype is SMN-dependent, and …
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Identifying The Roles of miR-17 in Ciliogenesis and Cell Cycle
… evidence suggests a significant contribution of primary cilia to cell division and proliferation mechanisms. MicroRNAs, especially miR-17, contribute to cell cycle regulation and proliferation. Recent investigations have highlighted the dysregulated expression of miR-17 in various malignancies, …
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Sensory Primary Cilium is a Distinct Signaling Compartment
<p>The primary cilium is a solitary cellular organelle that protrudes from the apical cell membrane. Findings on cilia-dependent mechanosenstation have shown that the primary cilium acts as a transducer of fluid-shear stress into intracellular signaling. Over recent years, studies in primary cilia …
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Consequences of Plk4 overexpression in mouse pancreatic organoids
… causes centrosome amplification, a reduction in primary cilia number, and increased proliferation of pancreatic endocrine α-cells and β-cells. This project aimed to investigate the consequences of Plk4 overexpression on pancreas cell biology using mouse pancreatic ductal organoids. In accord with …
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Mutation of Polaris, an Intraflagellar Transport Protein, Shortens Neuronal Cilia
Primary cilia are non-motile organelles having 9+0 microtubules that project from the basal body of the cell. While the main purpose of motile cilia in mammalian cells is to move fluid or mucus over the cell surface, the purpose of primary cilia has remained elusive for the most part. Primary cilia …
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The role of CD133 in mouse incisor tooth epithelium
… epithelium in CD133 KO mice revealed disturbed primary cilia homeostasis. Primary cilia are crucial in interpreting extracellular cues and CD133 KO primary cilia specifically fail to transmit Shh-mediated signals. Further investigation of the functions of CD133 revealed that CD133 is important …
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Transcriptional regulators in stem cell biology
… the mammary epithelium, EMT drives formation of primary cilia, which promote MaSC self-renewal. We showed that primary cilia enable MaSC function in part through inhibiting GLIS2. Glis2 deletion in mice led to MaSC expansion and increased self-renewal. Conversely, GLIS2 hyperactivity in mammary …
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A Novel Collapsing Response Mediator Protein 2 Binding Domain for Kinesin Light Chain 3
Significant similarity exists between primary cilia and sperm flagella. Both contain an axoneme and both require presence of ODF2. Therefore, primary cilia can be used as a partial model to study the assembly of sperm flagella. Recently, we have shown that Collapsin Response Mediator Protein 2 is …
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The role of sphingolipids in autosomal dominant polycystic kidney disease (ADPKD)
… cell proliferation. Dysregulation of their primary cilia (evident by decreased levels of acetylated α-tubulin) is also a crucial factor in defective renal cystic epithelia. Sphingolipids contribute to regulating these cellular processes. Two isoforms of sphingosine kinase (SK1 and SK2) …
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Leveraging cellular models of polycystic kidney disease for mechanistic discovery and therapeutic development
… Using RNA-sequencing, we identify instability of primary cilia, where the polycystins co-localize, that ultimately drives aberrant cell cycle re-entry in PKD cells. Stabilization of primary cilia, through inhibition of histone deacetylase 6-mediated ciliary disassembly, reduces PKD cystogenesis in …
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Cardiac repair post-myocardial infarction: Roles of the primary cilium and the long non-coding RNA Malat1
… factor Wilms’ tumor 1 (Wt1) and the primary cilium regulate EMT. Furthermore, the long non-coding RNA (lncRNA) Malat1 is not only known to regulate EMT, but also mitigates cell death from injury. Little is known about the roles of Wt1, primary cilia, and Malat1 in relation to neonatal …
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The role of extracellular matrix in modulation of myocyte Ca2+ signalling and contraction in the isolated rat heart
… study also looked at the evidence of presence of primary cilia in adult rat hearts and found no evidence of primary cilia in either ventricular tissue sections or isolated myocytes from the adult rat hearts. The effect of novel EPAC signalling pathway which contributes to the EC-coupling and Ca2+ …
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Functional Genomics Based Interrogation of Cell-Fate Determination Pathways
… Stk11 loss resulted in disassembly of the primary cilium, a cellular organizing center for Hh pathway components, thus dampening Hh signaling. Loss of Stk11 also induced aberrant signaling through the Wnt pathway. Chemicals that targeted the Wnt acyltransferase Porcupine or that restored …
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