Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 84 for “"Primary Cell"”.
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Developing cultivated mollusks through establishing primary cell culture methods of Eastern Oyster, Crassostrea virginica, as a model bivalve
… Cultivated seafood is made by growing animal cells in vitro using stem cells for edible food, eliminating the need to raise the entire animal. A crucial first step in developing cultivated seafood is creating a well-characterized cell line that can continuously grow and differentiate into …
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A NOVEL MAGNETIC THREE-DIMENSIONAL LEVITATED PRIMARY CELL CULTURE SYSTEM FOR THE DEVELOPMENT OF IN VITRO SALIVARY GLAND-LIKE ORGANOIDS
… to repair hypo-functional SG via various cell-based approaches in three-dimensional (3D) scaffold-based systems. The majority have nonhuman-based components. Therefore, this project aims to develop SG mini-glands/organoids using a novel 3D xeno-free system (M3DL) that assembles and …
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Biological Potencies and Mechanism of Action of Estrogens and Antiestrogens in Primary Cell Cultures of Human Endometrium and in Mcf-7 Human Breast Carcinoma Cells in Vitro
Primary cultures of human endometrial cells were used to examine the direct effects of estrogens on progesterone receptor levels in vitro. Cells, grown in Dulbecco's EMEM, showed maximal stimulation of progesterone receptor (PR, 7-fold) after a 3-4 d incubation with 10nM estradiol (E(,2)). The
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Analysing M. abscessus-macrophage interactions and investigating treatments for M. abscessus and S. aureus intracellular bacterial infections, using a continuously growing, primary cell model of lung alveolar macrophages
… alveolar macrophages (AMs), as sentinel immune cells of the airways, are key cells for the recognition of these invaders, and they are central to activating and orchestrating the host immune responses aimed at eliminating these pathogens. Consequently, these cells are also fundamental mediators …
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Assessing the temporal role of miR-200 loss in murine models of NSCLC
… deaths in the United States, with non-small cell lung cancer (NSCLC) making up a majority of new diagnoses. Metastasis is the big killer in NSCLC and is driven by epithelial-mesenchymal transition (EMT) and immune evasion. The microRNA 200 family is a master regulator of EMT and is implicated …
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COMPARATIVE ULTRASTRUCTURE OF APICAL CELLS AND DERIVATIVES IN BRYOPHYTES, WITH SPECIAL REFERENCE TO PLASMODESMATA
This study focused on the primary cell wall constituents and plasmodesmata (PD) density in three mosses and four liverwort apical cells (AC) and immediate derivatives. The three mosses have tetrahedral apical cells and the liverworts possess tetrahedral, hemidiscoid and lenticular AC geometries. …
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Steroid Stimulated Protein Synthesis in Fish Cell Cultures
Protein synthesis in Gonad primary cell cultures and Chinook Salmon Embryo cell cultures (CHSE-214) was stimulated by 17-beta-estradiol, 17-alpha-hydroxyprogesterone, and testosterone. Cells were treated for six hours with a 10-6 M concentration of steroid and radiolabelled with 35s-methionine for …
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Strategies to Target the Bone Morphogenetic Protein Signalling Pathway in Lung Disease
… of various molecules to human and murine cells lines, and to mouse primary cell isolates, MAECs, to examine the potential of the mechanism for adaption to a clinical mechanism for delivery. An aerosol delivery system was developed to utilise a current aerosol generator for the delivery of …
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ΧΗΜΕΙΑ ΤΟΥ ΠΡΩΤΟΓΕΝΟΥΣ ΚΥΤΤΑΡΙΚΟΥ ΤΟΙΧΩΜΑΤΟΣ ΚΑΙ ΜΕΤΑΒΟΛΕΣ ΤΩΝ ΣΥΣΤΑΤΙΚΩΝ ΤΟΥ ΚΑΤΑ ΤΗΝ ΟΝΤΟΓΕΝΕΣΗ ΤΩΝ ΚΑΡΠΩΝ ΚΑΙ ΤΗΝ ΩΡΙΜΑΝΣΗ ΤΟΥΣ
IT WAS STUDIED THE CHEMISTRY OF THE PRIMARY CELL WALL AND THE CHANGES OF ITS CONSTITUENTS IN THE FLESH OF PRUNUS INSITITIA, PRUNUS PERSICA AND PRUNUS ARMENIACA FRUITS. A PROPER EXPERIMENTAL METHODOLOGY WAS DEVELOPED FOR THE ISOLATION,SEPARATION, IDENTIFICATION AND QUANTITATIVE DETERMINATION OF EACH …
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Structure and dynamics of plant cell walls and membrane peptides from solid-state NMR
… of the architecture and loosening of primary plant cell walls and the interactions between membrane and peptides. Plant cell wall is a complex system mainly comprising three types of insoluble polysaccharides: cellulose, hemicellulose and pectin. The spatial arrangement of these …
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The Transcriptional Profile of Microglia: From Brain to Dish
… indicate that microglia are a pathogenic cell type in Alzheimer’s disease (AD). It is important to understand the transcriptional profiles of microglia, both from primary human cells and the in-vitro model systems used to study the cells at scale. In this thesis, I aim to build on previous …
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Cell-walls of growing plant cells
The plant primary cell wall is a three-dimensional interwoven network of cellulose microfibrils, cross-linked by xyloglucan and dispersed in a pectin matrix. It has been suggested that in the wall of growing plant cells, xyloglucan is bound to the rigid cellulose microfibrils by hydrogen bonds and …
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Pengaruh ekstrak pegagan (Centella asiatica linn.) terhadap pertumbuhan kultur primer sel hepar baby hamster yang dipapar 7.12- dimetilbenz(α)antrasen
… ENGLISH: Cancer is the growth and development of cells that are abnormal and uncontrollable. Environmental factors have been identified the cause of the occurrence of cancer in hepar derived from ingredients that are carcinogenic polycyclic aromatic compounds, such as hydrocarbons (PAH) i.e. 7.12- …
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Development and characterization of an in vitro culture system as a physiological model for chronic Hepatitis B infection
… cases of chronic hepatitis, cirrhosis, and hepatocellular carcinoma. Significant disadvantages exist among currently available therapeutics (e.g. IFNca, lamivudine, adefovir, etc.) that include limited efficacy and the promotion of drug-resistant viral strains. These therapeutics are the research …
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Environmental Contaminants and Respiratory Innate Immunity: In Vitro Effects of Polycyclic Aromatic Hydrocarbons and Crude Oil on Tracheal Antimicrobial Peptide Gene Expression.
… antimicrobial peptide (TAP) gene expression in primary cell cultures of bovine tracheal epithelial cells. It was found that exposure to either crude oil or PAHs was associated with suppression of TAP gene expression in cultures. However, the consistency of this result varied in significance …
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Dhhc5 Regulates Beta-Adrenergic Signaling In The Heart Through Palmitoylation of G Alpha Proteins
… palmitoylation/depalmitoylation cycles after cellular stimulation supporting a direct signaling role for this posttranslational modification. The work in this dissertation investigated whether β-adrenergic stimulation led to stimulus-dependent palmitoylation of downstream signaling proteins in …
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The Role of Perivascular Fibrosis In Post-Stroke Glymphatic Impairment and Cerebral Amyloid Angiopathy
… I hypothesize that an abnormal pattern of extracellular matrix deposition within the vascular basement membrane, termed fibrosis, impairs Aβ drainage from the aged brain after stroke. I further hypothesize that inhibition of astrocytic transforming growth factor-β (TGF-β) signaling can reverse …
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Characterisation of the expression profile and endothelial function of Rho GTPase RhoJ
… molecular switches that regulate many aspects of cell physiology. A number of Rho GTPases are essential for the formation of new vessels from pre-existing ones, a process known as angiogenesis. RhoJ/TCL belongs to the Cdc42 subfamily of Rho GTPases. Previous bioinformatic and primary cell line …
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Characterization of novel genetic alleles of cellulose synthase
Cellulose is the most abundant polymer on earth. Despite this, relatively little is known about the proteins and biochemical mechanisms governing cellulose biosynthesis. Only in the past 10 years since the sequencing of the Arabidopsis thaliana genome have we begun to unravel the full complexity of …
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Imaging Glioblastoma metabolism using mass spectrometry imaging
… (GB) is an inherently heterogenous and invasive primary brain tumour. Genetic and transcriptomic studies have attempted to classify GB into subtypes that can identify therapeutic vulnerabilities and predict survival, these include metabolic subtypes. An outstanding question and technical …
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