Global ETD Search
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Showing 1 to 10 of 10 for “"PrfA"”.
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Essential features of a PrfA-dependent : promoter of Listeria monocytogenes
… of well-known virulence genes are controlled by PrfA that belongs to the Crp-Fnr family of transcriptional activators. A PrfA-mediated transcription initiating at a virulence gene promoter, inlC promoter (PinlC) that regulates the expression of the small, secreted internalin C, was in-depth …
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Gene Knockout Of PRFA And The Arginine Deiminase Pathway In Listeria Monocytogenes
… system to help maintain homeostasis. The prfA gene, which codes for the PrfA protein, is considered to be a crucial virulence regulator in L. monocytogenes. The PrfA protein has the ability to bind to palindromic promoter boxes within the chromosome in order to recruit necessary components …
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CONTRIBUTIONS OF SIGMA-B AND PRFA TO STRESS RESPONSE AND VIRULENCE GENE EXPRESSION IN LISTERIA MONOCYTOGENES
… virulence gene expression in L. monocytogenes is PrfA. I measured expression of PrfA-dependent and sigmaB-dependent genes under conditions that activate each regulator, using quantitative reverse transcription-PCR (qRT-PCR). I found that sigmaB is active preferentially under environmental stress …
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Molekularbiologischer Nachweis und Immunologie des P60-Proteins, sowie In-vitro-Transkription PrfA-abhängiger bzw. -unabhängiger Gene von Listeria monocytogenes
… von Phly, PplcA und PactA erfolgt strikt PrfA-abhängig, während Piap, PprfA1/2 und - unerwarteter Weise - auch Pmpl PrfA-unabhängig transkribiert werden. Sie erfolgt - ausgenommen bei PprfA - nur bei ausreichender Verfügbarkeit von ATP nicht jedoch GTP. Um eine effiziente Transkription zu …
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Knockout of the trans-acting riboswitches in Listeria monocytogenes to disrupt downstream virulence factors
… factors that heavily rely on upregulation of the prfA gene. In this paper the pIMC plasmid is used in an attempt to knock-out the untranslated region (UTR) of the prfA gene located on the LIPI-1 pathogenicity island of the L. monocytogenes.</p>
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Genetische Organisation und Transkription eines Virulenz-assoziierten, instabilen Chromosomenabschnitts von Listeria ivanovii
… wurde für fast alle Internalingene eine PrfA-Box identifiziert und es bestätigte sich in dieser Arbeit, dass die meisten der Internalingene PrfA-abhängig exprimiert werden. Allerdings wiesen die einzelnen Gene kein einheitliches Transkriptionsprofil unter verschiedenen in …
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Antibiofilm properties of selected Lactobacillus species and their mechanism of action against Listeria monocytogenes
… how expression of the L. monocytogenes prfA gene is affected by the presence of CFS of LAB. The results demonstrated that the presence of all CFS of LAB (L. acidophilus La14 150B, L. plantarum and L. rhamnosus ATCC 4356) caused a significant downregulation in the expression of prfA (p < …
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Interaktion von Listeria monocytogenes mit Endothelzellen
… Gen, welches für den positiven Regulationsfaktor PrfA kodiert, deletiert, reduzierte dies die Invasionsrate beträchtlich. Listerienstämme mit einer Deletion im für InlB kodierenden Gen sind unfähig, HBMEC zu invadieren. Neben InlG und ActA spielt auch PrfA eine entscheidende Rolle bei der Invasion …
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Microbiology and cell biology of the interaction between Listeria monocytogenes and Acanthamoeba spp.
… that allowed GFP expression under control of the prfA promoter was used to assess the expression of Listeria virulence genes within amoeba cells. The role plasmid encoded genes in interactions of L. monocytogenes with amoebae was assessed using plasmid-cured versus wild type in the co-cultures. …
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Identification and characterization of the inlGHE gene cluster of Listeria monocytogenes
… transcribed extracellularly and independent of PrfA. To study the function of the inlGHE gene cluster several in-frame deletion mutants were constructed which lack the genes of the inlGHE cluster individually or in combination with other inl genes. When tested in the mouse model, the inlGHE …