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Showing 1 to 8 of 8 for “"Pre-steady state kinetics"”.

  1. Part I: Investigation of mechanism(s) of Cu(II) and Zn(II) neurotoxicity Part II: Pre-steady state kinetics of the reduction of cytochrome b561 with ascorbate

    Kollalpitiya, Konara Yamuna2011

    wichita-thes

  2. FLUORESCENCE SPECTROSCOPY AND PRE-STEADY STATE KINETIC STUDIES ON E. COLI METHIONYL TRANSFER RNA SYNTHETASE

    Suryadi, JimmyWake Forest University2011

    … thesis we utilize fluorescence spectroscopy and pre-steady state kinetics to unveil some features of MetRS catalysis that were previously unclear.

    wfu Repository record

  3. Mechanistic studies of the class I ribonucleotide reductases from S. cerevisiae and E. coli.

    Ge, Jie, 1976-Massachusetts Institute of Technology2003

    … considered. In the second part of this thesis, pre-steady state kinetics of the class I E. coli RNR is investigated using stopped-flow UV-visible spectroscopy and rapid chemical quench methods. These studies have led to a model in which the rate determining step in catalysis is a physical step …

    mit Repository record

  4. Kinetic and mutational studies of two RNA-interacting enzymes

    Casina, Veronica ChristineWake Forest University2011

    Cellular enzymes interact with RNA substrates at several key stages in an organism's life cycle, particularly during the processes of transcription and translation. A more detailed description of enzyme mechanistic features and kinetic parameters is important both for understanding molecular …

    wfu Repository record

  5. Biochemical mechanisms for selectivity governing RNA and DNA replication

    Dangerfield, Tyler LaneThe University of Texas at Austin2021

    … T7 DNA polymerase, the SARS-CoV-2 pandemic devastated the world. I used techniques developed in my previous studies to characterize the RNA dependent RNA polymerase (RdRp) from SARS-CoV-2, a prime target for antiviral drugs to stop replication of the virus. I developed methods to express the tag …

    texas Repository record

  6. Interactions of Smooth Muscle Myosin Filaments with Actin and Myosin Light Chain Kinase: Insights into the Mechanics of Smooth Muscle Contraction

    Haldeman, Brian D.2015

    … of the filamentous conformation of SMM on the kinetics underlying the interactions with MLCK and actin is unknown. The filamentous structure of unphosphorylated smooth muscle myosin is not stable under physiological conditions, so there is a lack of research studying filaments of smooth muscle …

    unr Repository record

  7. Exploring reactivity and component interactions in Toluene/o-Xylene Monooxygenase from pseudomonas sp. OX1

    Liang, Alexandria DelizMassachusetts Institute of Technology2015

    … the hydroxylase regulating substrate access and pre-organizing the diiron site for reactivity. The relationships between structure and reactivity that provide this control are reviewed for both three- and four-component BMMs, including soluble methane monooxygenase, phenol hydroxylase, toluene …

    mit Repository record

  8. Electron gating and mechanism of proton exit in quinol oxidation in cyt bc1 complex from R. sphaeroides

    Victoria, Doreen C.University of Illinois at Urbana-Champaign2010

    … transfer of quinol oxidation at the Qo-site. Previous studies showed that mutation at this position resulted to a substantially inhibited electron transfer, while the bypass rates were equal to or less than the wild-type strain. In addition, these strains showed resistance to stigmatellin and …

    uiuc Repository record