Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 34 for “"PrPSc"”.
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Characterizing the pro-inflammatory cytokine response by dendritic cells upon exposure to PrP proteins
… cell (DC) interaction with infectious prions (PrPSc) represents an important part of prion pathogenesis. Previous studies have shown that DCs are likely among the first immune cells to interact with PrPSc after oral exposure. This study looks at the initial exposure of PrPSc on DC activation by …
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Mechanisms of Prion Strain Interference
… fatal. The infectious agent in prion disease (PrPSc) is an abnormal isoform of an endogenous host protein (PrPC). Prion conversion involves a conformational change of PrPC into PrPSc, and this process may involve host polyanions such as ribonucleic acid (RNA). Conformational alterations in …
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The role of the 37-kDa/67-kDa laminin receptor in the cellular metabolism of the prion protein
… Erkrankungen, bei denen die abnormale Form (PrPSc) des zellulären Prion Proteins (PrPC) eine entscheidente Rolle spielt. PrPSc lagert sich im Gehirn von Menschen und verschiedenen Säugetieren zu langen Ketten, sogenanntem Amyloid, zusammen und bildet amyloide Plaques. Dies führt letzendlich …
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Exploring Novel Immunodiagnostics for Prion Disease
… misfolds, producing the infectious isoform PrPSc,which can readily self propagate with no nucleic acid intermediate. PrPSc aggregates are insoluble self-molecules, which results in a large number of limitations pertaining to the prevention, detection, and treatment of TSEs; including a lack …
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Molecular Basis of Prion Pathogenesis and Development of a Novel Therapeutic Strategy
… and infectious form of the prion protein (PrPSc). Previous reports have shown that PrPSc induces endoplasmic reticulum stress and changes in calcium homeostasis in the brain of affected individuals. My research shows that the calcium-dependent phosphatase Calcineurin (CaN) is hyperactivated …
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Proteins as markers of TSE infection in sheep blood
… in blood.<br/>Detection of PK-resistant PrPSc has been extensively used as a biochemical marker for TSE infectivity. However, when this project was started it was not known if PKresistant PrPSc was present in TSE-infected sheep blood in sufficient quantities to explain the infectivity …
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„Ex vivo” Replikation des pathogenen Prion Proteins
… Isoform (PrPC) in eine pathogene Isoform (PrPSc) verursacht. Diese Konformationsänderung kann entweder spontan oder induziert durch exogenes PrPSc erfolgen. Dabei kommt es zur Anlagerung von PrPSc an PrPC. Diese Erkrankung wirkt sich vor allem im Gehirn der betroffenen Organismen aus. Dabei …
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Probing Structural Differences of Recombinant Prion Isoforms Using Fluorescence Spectroscopy
… to an infectious, misfolded, fibrillar isoform, PrPSc, is responsible for various neurodegenerative diseases in a variety of mammalian hosts. Although the structure of PrPC is well studied, the structure of PrPSc is not known. Obtaining structural information on the misfolded isoform of prion may …
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Probing Structural Differences of Recombinant Prion Isoforms Using Fluorescence Spectroscopy
… to an infectious, misfolded, fibrillar isoform, PrPSc, is responsible for various neurodegenerative diseases in a variety of mammalian hosts. Although the structure of PrPC is well studied, the structure of PrPSc is not known. Obtaining structural information on the misfolded isoform of prion may …
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Neuropeptid Y- und Prionprotein- spezifische Aptamere
… ist die infektiöse Form des Prionproteins (PrPSc). Es wird angenommen, daß PrPC durch einen posttranslationalen Prozeß in PrPSc konvertiert werden kann. Trotz identischer Primärstruktur unterscheiden sich die beiden Prionproteinisoformen (PrPC und PrPSc) grundlegend in ihren biochemischen …
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Deciphering the Molecular Basis of the Species Barrier in Prion Disease Using Bank Vole PrP
… β-sheet rich conformer referred to as scrapie or PrPSc. The infectious nature of this conformer enables it to template the conversion of PrPC into additional copies of PrPSc, the efficiency of which depends on the sequence-encoded structural compatibility of the two molecules. PrPC and PrPSc from …
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Einfluss der Helix 1 und des β-Faltblattes auf die Aggregation des Prionproteins und seine Amyloidstruktur
… PrPC in seine pathologische Isoform PrPSc verursacht. In derzeitigen PrPSc-Strukturmodellen wird vermutet, dass die Helix 1 bevorzugt in ein β-Faltblatt bei der Aggregation des Prionproteins umfaltet. Diese Annahme wird durch die NMR-Struktur von nativem PrPC gestützt, da im Kontrast …
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Prions Propagate as Dynamic Strain Mixtures
… protein, PrPC, into the infectious conformation PrPSc. Phenotypic differences in disease (i.e., strains) have been observed for decades and are thought to be encoded by stain-specific conformations of PrPSc. There is evidence that prion strains exist as a mixture of multiple strains, containing a …
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Investigation of preferential binding properties of monoclonal antibodies M1:1D2 and H4:4 towards the conformers of recombinant prion protein
… of diagnosis and it may also allow detection of PrPSc in body fluids. In this project the two mAbs M1:1D2 (IgG3) and H4:4 (IgG2a) which were raised against prion protein purified from outdated human platelets were tested for their specificity against SDS aggregated recombinant human PrP peptide. …
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Molecular Basis of Mammalian Prion Protein Misfolding
… leading to the infectious PrP conformation (PrPSc) is not yet elucidated. Moreover, the structure of the pathogenic entity is unknown and a crucial involvement of accessory molecules within the infectious particle has recently gained strong evidence. Prions have raised not only health-related …
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Sistema nervoso enterico e scrapie sperimentale in ovini di razza sarda con diversa suscettibilità genetica nei confronti della malattia
… l’immunoreattività (IR) nei confronti della PrPSc, del “marker” panneuronale Hu C/D, dell’ossido-nitrico sintetasi (nNOS), della calbindina (CALB) e della proteina fibrillare acida gliale (GFAP). In 8 pecore con genotipo ARQ/ARQ, clinicamente sane e sacrificate a 12-24 mesi p.i., nonché in 5 …
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Effects of glycosylation on the structure and fibrillization of prion protein fragments
… that the structural transition from PrPc to PrPSc is the major event leading to transmissible spongiform encephalopathies. Although the mechanism of this transition remains elusive, glycosylation has been proposed to impede the PrPc to PrPSc conversion. Structural studies on glycoprotein …
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Prion protein in health and disease
… into its pathogenic conformation, termed PrPsC. The role of PrPSC in the pathogenesis of prion diseases, or transmissible spongiform encephalopathies, has been studied intensively yet the mechanism by which PrP misfolding in neurons leads to injury and death remains enigmatic. Much less …
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Doxycyclin bei der sporadischen Creutzfeldt-Jakob-Krankheit
… die Ablagerung von pathologischem Prionprotein (PrPSc) angenommen, die zu Funktionsausfall und Zelltod im zentralen Nervensystem führt. Charakteristisch sind eine rasch progrediente Demenz, zerebelläre Ataxie, Myoklonien und der akinetische Mutismus. Bisher ist keine kausale Therapie bekannt und …
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Dilations of the Endoplasmic Reticulum Contribute to Spongiform Degeneration and Unlock a Door to a Unified Model of Neuropathological Features in Prion Diseases
… neuronal loss, and misfolded prion protein (PrPSc) deposition. The mechanistic origins of spongiform degeneration, which manifests as intracellular vacuolation in neurons, have remained unclear. We considered similarities between the spongiform degeneration in prion diseases and vacuolation …
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