Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 10 of 10 for “"Pou5F1"”.
-
EWSR1 and FUS fusion genes in tumorigenesis
… cancer-associated gene. In Article II, the EWSR1-POU5F1 fusion was found in hidradenoma and mucoepidermoid carcinoma, the first report of EWSR1 fusion genes in epithelial tumor types. The EWSR1-POU5F1 chimera was a stronger transcriptional activator than wild-type (wt) POU5F1, analogous to other …
-
Quantification of gene expression in single cells
… stem cells involves the transcription factors Pou5f1, Nanog and Sox2. We quantified their expression in single stem cells and observed that they are not correlated with each other. Instead Pou5f1 correlates with the transcription factors Id1 and Id3. We conclude that quantitative gene …
-
Molecular mechanisms controlling the expression of the HLA-Cw*06 psoriasis susceptibility allele
… was detected at a fifth CGI, lying upstream of POU5F1. To complement these data, a methylation profile of the entire PSORS1 locus was generated in two HLA Cw*0602,-Cw*0702 heterozygous individuals. <br/><br/>This showed that the majority of methylated cytosines mapped between the POU5F1 and …
-
Identification of Undifferentiated Human Embryonic Stem Cell-Specific Genes
… Recent reports showed that several genes such as Pou5F1 (Oct4, Oct3/4), Sox2, Nanog, LIN28, KLF4, Myc(c-Myc) maintained a pluripotent undifferentiated state. As we know, both stem cells and cancer cells have self-renewal ability. To investigate for the mechanism that maintain the self-renewal and …
-
Characterization of Genes Specifically Expressed in Embryonic and Cancer Stem Cells
… three germ layers. The stem cell genes such as Pou5F1 (Oct4, Oct3/4), Sox2, Nanog, LIN28, KLF4, Myc (c-Myc) act alone and interact with each other for mainintaining the stem cell state. These genes have been already studied well. The emergence of new genes and the interaction with new genes can …
-
The role of pou2/spiel-ohne-grenzen (spg) in brain and endoderm development of the zebrafish, Danio rerio
… pou2 is the orthologue of the mammalian Oct4 (Pou5F1) gene. I find by detailed expression and transplantation analysis that pou2 is cell autonomously required within the neuroectoderm to activate genes of the MHB and hindbrain primordium, like pax2.1, wnt1, gbx2 or krox20. By gain-of-function …
-
Tumor-like growth of mouse embryonic stem cells
… levels of the transcription factor Oct3/4 (Pou5f1), a master regulator of pluripotency in mouse ESCs, were monitored. The mouse ESCs maintained in these conditions showed similar levels of Oct3/4 expression, but no endogenous Shh expression. However, intriguingly, the mouse ESCs maintained …
-
Isolation, characterisation and in vitro potential of Oogonial stem cells
… mRNA and protein level, including LIN28, NANOG, POU5F1 (OCT4), IFITM3 (fragilis), STELLA, PRDM1 (BLIMP1), and C-KIT, indicating their early germline nature. Investigation of neo-oogenesis potential revealed that putative human OSCs were associated rarely with fetal somatic cells in primordial …
-
Genesis of the human germline
… SOX17 and maintenance of pluripotency factors, POU5F1 and NANOG. I also explored PGC competence and specification using highly resolved single-cell transcriptomic profiling of our in vitro PGC-like cells (PGCLC). Using an integrative analysis with existing human and primate embryonic datasets …
-
Oct4 and Sox2 regulate methylation at the Igf2/H19 imprinting control region
Mono-allelic expression of H19 and Igf2 depends on parental-specific methylation of an imprinting control region (ICR). Although CTCF sites maintain the absence of DNA methylation on maternal ICRs after fertilization, some Beckwith-Wiedemann patients showed maternal ICR methylation that correlated …