Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 54 for “"Post-Translational Modifications (PTMs)"”.
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Disentangling bacterial virulence mechanisms: Klebsiella pneumoniae targets post-translational modifications (PTMs) to manipulate innate immunity
… mechanisms, including the targeting of host post-translational modifications (PTMs), to attenuate inflammation. The Bengoechea laboratory has previously demonstrated that Klebsiella triggers a type I interferon (IFN) response in vivo and here it is shown Klebsiella targets ISGylation, a type …
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Algorithms to Detect Motifs and Predict Post-Translational Modification Sites
Post-translational modifications (PTMs) are an important step in the formation of a mature protein. While much is known about some PTMs such as Phosphorylation, there are other PTMs such as Cysteine S-sulfenylation that are currently being researched and analyzed. For this project, an algorithm, …
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Development of Dendrimer-based Technologies for Phosphoproteomics and Glycoproteomics in Biomarker Discovery
The dynamics of post-translational modifications (PTMs) of proteins keeps cells adapting to the ever-changing microenvironment by regulating protein structure and function in the real-time mode. Of all PTMs, protein phosphorylation and glycosylation are undoubtedly two of the most common and …
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NEW METHODS TO QUANTIFY METHIONINE OXIDATION AND PROTEIN GLYCOSYLATION AND A DE NOVO GLYCOPEPTIDE DECOY GENERATOR
Protein post-translational modifications (PTMs) produce complex, heterogeneous species that can best be identified and analyzed using mass spectrometry (MS). The ever-growing number of protein bioetherapeutics need effective MS tools to characterize PTMs and ensure drug safety and efficacy. …
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Microtubule Dynamics in Neuronal Morphogenesis
… of microtubules is determined in large part by post-translational modifications (PTMs), and regulation of disassembly factors. Consequences of perturbed microtubule dynamics include failure to specify the axon and dendrite of neurons, and can lead to neurological defects due to failed neuronal …
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Regulation of pyruvate kinase and glycerol-3-phosphate dehydrogenase in the freeze tolerant goldenrod gall fly: role in polyol cryoprotectant production
… A primary focus was the potential for reversible post-translational modifications (PTMs) to regulate enzyme activity. Several PTMs were analyzed for PK and G3PDH from control and freeze-exposed gall fly larvae using dot-blot analysis. Results showed that low and high phosphate forms of each enzyme …
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Computational analysis of cooperation and competition mechanisms between RNA-binding proteins
… levels, namely epigenetic, transcriptional, post-transcriptional, translational and post-translational. The post-transcriptional regulatory layer accounts for all the events taking place between transcription and translation and involves a myriad of trans and cis factors densely …
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Protein LC-MS Using Slip Flow Chromatography
… proteins with large numbers of variants and post-translational modifications (PTMs). PTM levels of histones are known to correlate with different stages of cancer. Due to the lack of resolution on intact histone separations, conventional methods for histone analysis require time-consuming …
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Proteomic Identification of Histone Post-Translational Modifications Induced By Dna Double-Strand Breaks and Novel Proteins Involved In The Dna Damage Response
… of unbiased systems for identifying proteins and post-translational modifications (PTMs) involved in DSB repair. In this work, we established a novel method, termed DSB-ChAP-MS (Double Strand Break-Chromatin Affinity Purification with Mass Spectrometry), for the affinity purification of a …
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Developing a Contextual Annotation Framework for Short Linear Motifs in Proteins
… metrics, including sequence conservation, post-translational modifications (PTMs), structural context derived from AlphaFold model scores, and the proximity of neighboring motifs. We evaluate each of these metrics using a test dataset sampled from the Eukaryotic Linear Motif (ELM) protein …
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Regulation and Function of Zeb1 Acetylation In Lung Adenocarcinoma Progression and Metastasis
… molecular weights, which have been attributed to post-translational modifications (PTMs). In the presented dissertation, I specifically addressed the gap in the molecular mechanisms by which PTMs of ZEB1 regulate its ability to induce EMT and how its activity might be therapeutically targeted. In …
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Post-translational modifications of SEL24K from salmon eggs and ZPA from Xenopus laevis eggs
<p>Post-translational modifications (PTMs) of proteins play significant roles in regulation of biological activities and signal transduction. Examining their diversity is critical for understanding the mechanisms of cellular regulations. Among the various techniques employed for identification of …
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Discovery and Characterization of Methylation of Arginine 42 on Histone H3: A Novel Histone Modification with Positive Transcriptional Effects
… DNA in forming the nucleosomal particle. Histone post-translational modifications (PTMs) lead to downstream effects indirectly by allowing or preventing docking of effector molecules, or directly by changing the intrinsic biophysical properties of local chromatin. To date, little has been done to …
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Towards targeted post-translational modification of endogenous proteins in complex environments
… amino acid side chains, brought about by co- and post-translational modifications (PTMs). Depending on the roles of the modified amino acids and the nature of the chemical changes, PTMs can have tremendous influence on protein function. This phenomenon can be exploited pharmacologically, as …
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Chemical Mutagenesis for the Modulation and Study of Protein Function and Dysfunction within Alzheimer’s Disease
Chemical mutagenesis is defined as the post-expression interconversion of amino-acid (AA) sidechains. This approach enabled the first ever point-mutations in proteins and has seen a resurgence of interest over the last two decades. This thesis reports on two disparate applications of chemical …
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Regulation of DHX16-mediated Innate Immunity by Unanchored Polyubiquitin
… organism. This process is tightly controlled by post-translational modifications (PTMs), like unanchored (or free) polyubiquitin (poly-Ub), which properly regulate IFN-I to elicit responses as needed. Despite its known roles regulating critical IFN-I pathways, the extent to which unanchored …
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Characterization of Glycans Derived from Biological Samples Using LC-MS/MS
… biological activities. One of the most prevalent post-translational modifications (PTMs) is protein glycosylation, which enhances several biological functions, such as protein stability, localization, cellular communication, inflammation, immune responses, and host-pathogen interactions. Unusual …
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Structural Insights into the Mechanisms Controlling the Modification and the Readout of Histone H3.1
… the tail) of histone H3.1 is decorated by many post-translational modifications (PTMs). These PTMs serve as epigenetic signals coordinating essential nuclear processes such as transcription, replication and DNA damage repair mechanisms. These marks can be reversibly deposited by specialized …
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Functional Importance of Oxidative Post-Translational Modifications of Ryanodine Receptor in Cardiac Sarcoplasmic Reticulum Ca2+ Handling During Oxidative Stress
… been done to specifically characterize oxidative post-translational modifications (PTMs) of RyR2 as a result of oxidative stress within the ischemic or failing myocardium. Commonly observed in many disease phenotypes, including cardiovascular disease, is the prominence of glutathione …
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Palmitoylation and Glutathionylation of Key Sarcolemmal Signalling Proteins in Cardiac Muscle
… are susceptible to a variety of different post-translational modifications (PTMs), such as the addition of a 16-carbon fatty acid palmitate known as palmitoylation and the addition of the tripeptide glutathione, known as glutathionylation. Palmitoylation can regulate many processes such as …
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