Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 10 of 10 for “"Pore-forming toxin"”.
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Understanding gene expression with a pore forming toxin
… the virulent attack of low doses of a well-known pore forming toxin (staphylococcal α-hemolysin (αHL)) and to develop a new biotech application using the same protein.
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Innate Immunity to Listeria monocytogenes Infection: Regulation of Dendritic Cell and Neutrophil Responses by MyD88 and the Type I IFN Receptor
… gains access to the host cell cytosol via the pore forming toxin listeriolysin O (LLO) and infects a variety of cell types including dendritic cells (DC). Our laboratory and others have shown that optimal DC maturation occurs when <italic>Listeria</italic> gain access to the host cytosol, and …
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Impedance spectroscopy for cellular and biomolecular analysis
… thesis describes a microfabricated device for performing continuous impedance analysis of individual cells held in an array of hydrodynamic traps. This device enables continuous long-term analysis of cells and time-dependent measurement of changes elicited by cytotoxic agents and drug compounds. …
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Understanding Type A Clostridium perfringens in Foal Necrotizing Enteritis and Canine Acute Hemorrhagic Diarrhea
… These strains produce three novel putative toxins related to the beta-sheet pore-forming toxin family, which were designated NetE, NetF, and NetG. Our group demonstrated that NetF is likely the major virulence factor in strains responsible for canine acute hemorrhagic diarrhea and foal …
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Staphylococcus aureus Panton-Valentine leukocidin (PVL) alters human neutrophil viability and function
… pathogenesis--if any--remains unknown. PVL is a pore-forming toxin with specificity for myeloid cells, including polymorphonuclear leukocytes (PMNs or neutrophils). It is widely assumed that PVL-mediated pore formation in the plasma membrane of PMNs leads ultimately to cytolysis. However, this …
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Staphylococcus aureus Panton-Valentine leukocidin (PVL) alters human neutrophil viability and function
… pathogenesis--if any--remains unknown. PVL is a pore-forming toxin with specificity for myeloid cells, including polymorphonuclear leukocytes (PMNs or neutrophils). It is widely assumed that PVL-mediated pore formation in the plasma membrane of PMNs leads ultimately to cytolysis. However, this …
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Enzyme-mediated labeling of proteins and protein-protein interactions in vitro and in living cells
… We then validated previous reports that the pore-forming toxin streptolysin 0 be used to deliver quantum dots to the cytosol of living cells. Lipoic acid ligase, or LpIA, has been previously applied to site-specific protein labeling of peptide-tagged proteins using small molecule probes …
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Paviršiuje imobilizuotų membranų formavimosi ir sąveikos su α-hemolizinu dinamikos tyrimas greitosios Fourier transformacijos elektrocheminio impedanso spektroskopijos metodu /
… and the dynamics of the incorporation of the pore-forming toxin, α-hemolysin, into tBLM were investigated using FFT-EIS. FFT-EIS is unique in that it allows to measure electrochemical impedance spectra very rapidly, within ∼15 s (measuring spectra in the 0.2-10 kHz AC range). In the case of …
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Novel strategies to prevent and treat experimental pneumococcal disease
… specific protection. Pneumolysin, the pore-forming toxin produced by S. pneumoniae, may play a role in future pneumococcal vaccine. It is a major virulence factor produced by all invasive isolates and has previously been demonstrated to confer non- serotype specific protection. In this …
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Host lncRNAs recognize invading bacteria and aid against infection by associating with polysomes
… of these strains, I explored whether the pore forming toxin, LLO, is able to trigger a host toxin-specific translational controls. To address this question, I massively employed polysome profiling, a classical approach to study translation and Next Generation Sequencing (NGS) to monitored …