Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 74 for “"Pore-forming"”.
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Understanding gene expression with a pore forming toxin
… the virulent attack of low doses of a well-known pore forming toxin (staphylococcal α-hemolysin (αHL)) and to develop a new biotech application using the same protein.
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Dynamics and Model of the Pore-Forming Protein Lysenin
… fragility and instability. However, lysenin, a pore-forming protein extracted from the earthworm Eisenia foetida, provided a unique opportunity to study a protein which is stable in both a soluble and membrane phase. Lysenin channels possess several important properties characteristic of ion …
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The role of a pore-forming protein, Mpeg1, in cytosolic import
Cross-presentation is a process by which professional antigen presenting cells acquire exogenous antigens and present them on their surface on MHC class I molecules. This process is key for the initiation of CD8+ T cell responses. Two main pathways for cross-presentation have been described, …
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Novel Preparation of Porous Alumina using Ice Particles as Pore-Forming Agents
… study introduces the use of ice particles as pore forming agents to fabricate porous materials. This novel method possesses several advantages over current industrial techniques including environmental friendliness, low cost, and flexibility in size and shape of resulting pores. Porous ceramic …
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Molecular Interactions of Human C9: The Pore Forming Protein of the Complement System
… non-enzymatic assembly of C5b, C6, C7, C8, C9 forming C5b-9<sub>n</sub> (n=12-18), a cytolytically active pore-like structure known as the membrane attack complex (MAC). C8 is a heterotrimer comprised of a disulfide linked C8α-γ dimer that is non-covalently associated with C8β. Following …
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Cholesterol-Dependent Cytolysins and Perforin: Similar Pore-Forming Mechanisms in Pathogenic Attack and Human Immune Defense
MACPF/CDCs proteins are a huge family of pore-forming proteins present from the bacteria to the human genera. Cholesterol-dependent cytolysins (CDCs) are a family of toxins that participate in bacterial infection pathway at the membrane level. Great interest in this family is due to their …
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Characterization Of Streptolysin O As The Translocator For The Cytolysin-Mediated Translocation Process In Streptococcus Pyogenes
… <italic>Streptococcus pyogenes</italic> uses the pore-forming protein <underline>s</underline>trepto<underline>l</underline>ysin <underline>O</underline>: SLO) to translocate the <italic><underline>S.</underline></italic> <italic><underline>p</underline>yogenes</italic> …
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The electrophysiological and molecular effects of chronic beta-adrenoceptor antagonist therapy on human atrium
… as a result of altered expression of ion channel pore-forming and accessory subunits. Human atrial myocytes were isolated enzymatically from right atrial appendage tissue obtained from consenting patients, in sinus rhythm, undergoing cardiac surgery. Using whole cell patch clamping, K+ currents …
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Innate Immunity to Listeria monocytogenes Infection: Regulation of Dendritic Cell and Neutrophil Responses by MyD88 and the Type I IFN Receptor
… gains access to the host cell cytosol via the pore forming toxin listeriolysin O (LLO) and infects a variety of cell types including dendritic cells (DC). Our laboratory and others have shown that optimal DC maturation occurs when <italic>Listeria</italic> gain access to the host cytosol, and …
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The role of the cytolytic mediators, granulysin and perforin, in tuberculosis
… an antimicrobial protein, and perforin, a pore-forming molecule, have been found to co-localise in the granules of these two cell types. Granulysin has been shown to be directly cytotoxic to extracellular Mycobacterium tuberculosis (M.tb) and, together with perforin, is cytolytic against …
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Isolation and characterization of channel-forming proteins in the outer membrane of E. coli and Borrelia species
In this study pore forming proteins of the gram-negative bacteria B. burgdorferi, B. duttonii and E.coli were investigated. Therefore the study is subdivided into three parts. In the first part outer membrane preparation of three relapsing fever Borrelia were investigated. In the second part the …
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Structural and functional studies of type three secretion virulence factors from gram-negative pathogenic bacteria
… is composed of a tip protein, IpaD, and two pore-forming translocators, IpaB and IpaC. Maturation of the needle-tip complex proceeds in a stepwise manner. IpaD is at the tip of the nascent TTSA needle where it controls the first step of TTS activation. The bile salt deoxycholate (DOC) binds …
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A thermal/thermo-mechanical and imaging study of component distribution and interaction in pharmaceutical film coats
… films incorporated with various plasticizers and pore forming agents, hence to understand the drug release mechanisms in relation to the physical characteristics of EC films. The thermal properties of EC powder and EC films were initially characterized by means of thermogravimetric analysis (TGA), …
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Dynamics and Structures of Phospholipid Assemblies
… reveal their interactions with nanoparticles and pore-forming peptides. Nanoparticle-induced pearling and peptide-induced raft budding of giant lipid vesicles provide facile strategies to reproduce cellular shape transformations in vitro . These studies bridge the cell biology with materials, …
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Holin-dependent secretion of a large clostridial cytotoxin by C. perfringens
The clostridia are gram-positive, spore-forming, anaerobic pathogens capable of causing various diseases in both humans and animals. To do so, clostridial pathogens secrete a number of lethal toxins each associated with specific diseases. One family of proteins secreted by the clostridia are the …
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Molecular and functional study of splice variations of the L-type voltage-gated calcium channel CaV1.2 alpha1-subunit
… enriches the functional diversity of the pore-forming CaV1.2 alpha-1 subunit. Systematic screening of the human CaV1.2 alpha-1 gene by the transcript-scanning method revealed 19 of the 55 exons were subjected to alternative splicing, and two of these were novel exons. A large IVS3-S4 …
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Deciphering the regulation of perforin-2-mediated endocytic escape in dendritic cells
… to my project, the Kozik lab had identified the pore-forming protein perforin-2 as a dedicated effector of endocytic escape that allows cytosolic entry of exogenous antigen during cross-presentation. Perforin-2 is a member of the membrane attack complex and perforin superfamily (MACPF) and, …
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Development of Innovative Ultra-Filtration Fibre Technologies for Waste-Water Treatment in Membrane Bio-Reactors (MBR)
… high strength performance. Further variations in pore-former content will provide a route towards optimising membrane surface porosity, translating into potential improvements in fibre permeability and anti-fouling propensity. Three different experimental approaches were implemented to assess the …
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Structural characterization of chaperone and substrates from the type three secretion system of bacterium aeromonas hydrophilla
… they are the secretion needle subunit, AscF and pore forming translocators, AopB and AopD. Prior to secretion, AopB and AopD are protected by their intra-cellular chaperone AcrH, while AscF is bound to its heterodimeric chaperones AscE-AscG. In this work, we have solved the crystal structure of …
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Protonation and Membrane Protein Function
… is described, specifically for the study of pore forming membrane proteins.
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